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AB137098

Anti-GLO1 antibody [EPR8878]

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(8 Publications)

Rabbit Recombinant Monoclonal GLO1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 8 publications.

View Alternative Names

Lactoylglutathione lyase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase, Glx I, GLO1

3 Images
Western blot - Anti-GLO1 antibody [EPR8878] (AB137098)
  • WB

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Western blot - Anti-GLO1 antibody [EPR8878] (AB137098)

All lanes:

Western blot - Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

Lane 1:

Molt-4 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

TF-1 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

Red blood cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 21 kDa

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Flow Cytometry (Intracellular) - Anti-GLO1 antibody [EPR8878] (AB137098)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-GLO1 antibody [EPR8878] (AB137098)

Intracellular flow cytometric analysis of permeabilized Molt-4 cells labelling GLO1 with ab137098 at 1/10 dilution (red). Negative Rabbit IgG shown in green.

Western blot - Anti-GLO1 antibody [EPR8878] (AB137098)
  • WB

Lab

Western blot - Anti-GLO1 antibody [EPR8878] (AB137098)

Lanes 1 - 4 : Merged signal (red and green). Green - ab137098 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab137098 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab137098 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

GLO1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

HepG2 whole cell lysate at 20 µg

Predicted band size: 21 kDa

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  • Carrier free

    Anti-GLO1 antibody [EPR8878] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8878

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GLO1 protein also known as glyoxalase I plays an important role in cellular metabolism by detoxifying methylglyoxal a byproduct of glycolysis. It catalyzes the conversion of methylglyoxal into S-D-lactoylglutathione using glutathione as a cofactor. This enzyme has a mass of approximately 29 kDa and is expressed in various tissues throughout the body with higher levels found in the liver and kidney. Glyoxalase I (GLO1) expression is increased in response to stress conditions indicating its role in cellular defense mechanisms.
Biological function summary

Glyoxalase I is important for maintaining cellular homeostasis by mitigating harmful compounds. It forms part of the glyoxalase system which includes glyoxalase II functioning downstream of GLO1. This system prevents the accumulation of advanced glycation end-products (AGEs) which are implicated in cellular aging and dysfunction. By reducing the levels of methylglyoxal GLO1 supports normal physiological processes and protects cellular integrity.

Pathways

Glyoxalase I is an important component of the glyoxalase pathway. This pathway integrates into the detoxification network working alongside other important enzymes such as superoxide dismutase and catalase. These relationships highlight its function in cellular oxidative stress responses. GLO1's activity impacts pathways related to glycolysis and overall energy metabolism due to its role in reducing metabolic byproducts.

GLO1 has significant implications in diabetic complications and cancer. In diabetes increased methylglyoxal can worsen tissue damage making GLO1's role protective against advanced glycation end-product formation. In cancer GLO1 is overexpressed contributing to cellular proliferation and survival. Its interaction with proteins such as Hsp70 in cancer illustrates the adaptive mechanisms that tumor cells use for progression and chemoresistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione (PubMed : 20454679, PubMed : 23122816, PubMed : 9705294). Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B (PubMed : 19199007). Required for normal osteoclastogenesis (By similarity).
See full target information GLO1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:200 PubMed40571693

2025

D-lactate and glycerol as potential biomarkers of sorafenib activity in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Pedretti,Francesca Palermo,Miriana Braghin,Gabriele Imperato,Pasquale Tomaiuolo,Meral Celikag,Marta Boccazzi,Veronica Vallelonga,Lorenzo Da Dalt,Giuseppe Danilo Norata,Giorgia Marisi,Ilario Giovanni Rapposelli,Andrea Casadei-Gardini,Serena Ghisletti,Maurizio Crestani,Emma De Fabiani,Nico Mitro

Cells 14: PubMed40136646

2025

Depletion of MGO or Its Derivatives Ameliorate CUMS-Induced Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Ke Dong,Yun Zhao,Xue Wang,Zhaowei Sun,Fang Xie,Lingjia Qian

Molecular cell 84:2036-2052.e7 PubMed38688279

2024

Unprocessed genomic uracil as a source of DNA replication stress in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sneha Saxena,Christopher S Nabel,Turner W Seay,Parasvi S Patel,Ajinkya S Kawale,Caroline R Crosby,Helene Tigro,Eugene Oh,Matthew G Vander Heiden,Aaron N Hata,Zucai Suo,Lee Zou

Cell reports 32:108160 PubMed32966793

2020

Phosphorylation of T107 by CamKIIδ Regulates the Detoxification Efficiency and Proteomic Integrity of Glyoxalase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jakob Morgenstern,Sylvia Katz,Jutta Krebs-Haupenthal,Jessy Chen,Alireza Saadatmand,Fabiola Garcia Cortizo,Alexandra Moraru,Johanna Zemva,Marta Campos Campos,Aurelio Teleman,Johannes Backs,Peter Nawroth,Thomas Fleming

eLife 9: PubMed32795389

2020

ALKBH7 mediates necrosis via rewiring of glyoxal metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitanya A Kulkarni,Sergiy M Nadtochiy,Leslie Kennedy,Jimmy Zhang,Sophea Chhim,Hanan Alwaseem,Elizabeth Murphy,Dragony Fu,Paul S Brookes

Journal of leukocyte biology 109:605-619 PubMed32678947

2020

Classically activated mouse macrophages produce methylglyoxal that induces a TLR4- and RAGE-independent proinflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Prantner,Shreeram Nallar,Katharina Richard,David Spiegel,Kim D Collins,Stefanie N Vogel

Translational psychiatry 9:260 PubMed31624233

2019

Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tang,Haojun Huang,Shuiming Li,Mi Zhou,Zhao Liu,Rongzhong Huang,Wei Liao,Peng Xie,Jian Zhou

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 40:1182-1192 PubMed31366299

2019

Empathy in stroke rats is modulated by social settings.

Applications

Unspecified application

Species

Unspecified reactive species

Kazutaka Shinozuka,Naoki Tajiri,Hiroto Ishikawa,Julian P Tuazon,Jea-Young Lee,Paul R Sanberg,Sydney Zarriello,Sydney Corey,Yuji Kaneko,Cesario V Borlongan
View all publications
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