Anti-GLO1 antibody - N-terminal

• WB

Supplier Data

Western blot - Anti-GLO1 antibody - N-terminal (AB226353)

All lanes:

Western blot - Anti-GLO1 antibody - N-terminal (ab226353) at 0.1 µg

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 21 kDa

true

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - Anti-GLO1 antibody - N-terminal (AB226353)

GLO1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226353 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226353 at 0.4 μg/ml.

Lane 1 : ab226353 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-GLO1 antibody - N-terminal (ab226353)

Predicted band size: 21 kDa

false