AB154108

Anti-GLO2 antibody

Name: Anti-GLO2 antibody (ab154108) | Abcam
Brand: Abcam Limited
SKU: AB154108

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(7 Publications)

Rabbit Polyclonal GLO2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HAGH aa 50 to C-terminus.

View Alternative Names

GLO2, HAGH1, HAGH, Glyoxalase II, Glx II

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO2 antibody (AB154108)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO2 antibody (AB154108)

Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling GLO2 with ab154108 at 1/100 dilution.

Unknown

Western blot - Anti-GLO2 antibody (AB154108)

12% SDS PAGE

All lanes:

Western blot - Anti-GLO2 antibody (ab154108) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 29 kDa

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Western blot - Anti-GLO2 antibody (AB154108)

10% SDS PAGE

All lanes:

Western blot - Anti-GLO2 antibody (ab154108) at 1/1000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HAGH aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16775

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyoxalase 2 also known as GLO2 is an important enzyme in the glyoxalase system. The protein's molecular mass is approximately 29 kDa. It catalyzes the conversion of S-D-lactoylglutathione to glutathione and D-lactic acid allowing the detoxification of methylglyoxal a byproduct of glycolysis. GLO2 is expressed in many tissues with higher levels in liver and kidney reflecting its importance in detoxifying processes.

Biological function summary

Glyoxalase 2 has a significant role in cellular detoxification and maintenance of redox balance. As part of the glyoxalase system it closely works with another enzyme glyoxalase 1 to detoxify methylglyoxal. Through this interaction GLO2 helps prevent harmful effects caused by excessive levels of advanced glycation end-products (AGEs) protecting cells from potential damage.

Pathways

Glyoxalase 2 is involved in the methylglyoxal degradation pathway within the cellular glucose metabolism process. Specifically it partners with glyoxalase 1 in this detoxification route. Additionally GLO2 contributes to glutathione metabolism where it plays a critical part alongside glutathione peroxidases which further regulate oxidative stress.

GLO2 is associated with diabetes and neurodegenerative diseases. Altered levels of glyoxalase 2 impact methylglyoxal levels and have implications in diabetic complications through protein glycation. Furthermore studies indicate its potential link with Alzheimer's disease where oxidative stress and AGEs accumulation play central roles. GLO2's connection with glyoxalase 1 remains significant as both participate in these pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

See full target information HAGH

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Metabolites 14: PubMed38668337

2024

Accumulation of Non-Pathological Liver Fat Is Associated with the Loss of Glyoxalase I Activity in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Peter,Erwin Schleicher,Elisabeth Kliemank,Julia Szendroedi,Alfred Königsrainer,Hans-Ulrich Häring,Peter P Nawroth,Thomas Fleming

Biochemistry and biophysics reports 28:101138 PubMed34584990

2021

Generation of a GLO-2 deficient mouse reveals its effects on liver carbonyl and glutathione levels.

Applications

Unspecified application

Species

Unspecified reactive species

Xingsheng Li,Sonia Fargue,Anil Kumar Challa,William Poore,John Knight,Kyle D Wood

Antioxidants (Basel, Switzerland) 9: PubMed32942589

2020

Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Di Emidio,Francesco Rea,Martina Placidi,Giulia Rossi,Domenica Cocciolone,Ashraf Virmani,Guido Macchiarelli,Maria Grazia Palmerini,Anna Maria D'Alessandro,Paolo Giovanni Artini,Carla Tatone

Cells 9: PubMed31947651

2020

Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Di Emidio,Martina Placidi,Francesco Rea,Giulia Rossi,Stefano Falone,Loredana Cristiano,Stefania Nottola,Anna Maria D'Alessandro,Fernanda Amicarelli,Maria Grazia Palmerini,Carla Tatone

Cells 8: PubMed30781346

2019

Antiglycative Activity and RAGE Expression in Rett Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Cordone,Alessandra Pecorelli,Mascia Benedusi,Silvano Santini,Stefano Falone,Joussef Hayek,Fernanda Amicarelli,Giuseppe Valacchi

Biochimica et biophysica acta. Molecular basis of 1865:1389-1401 PubMed30771486

2019

SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary.

Applications

Species

Mouse

Giovanna Di Emidio,Silvano Junior Santini,Anna Maria D'Alessandro,Antonella Vetuschi,Roberta Sferra,Paolo Giovanni Artini,Gaspare Carta,Stefano Falone,Fernanda Amicarelli,Carla Tatone

Human molecular genetics 27:837-852 PubMed29309586

2018

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Applications

Unspecified application

Species

Unspecified reactive species

Ki Soon Kim,Nunziata Maio,Anamika Singh,Tracey A Rouault

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GLO2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO2 antibody (AB154108)

Western blot - Anti-GLO2 antibody (AB154108)

Western blot - Anti-GLO2 antibody (AB154108)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise