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AB242089

Anti-GLOD4 antibody

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(1 Publication)

Rabbit Polyclonal GLOD4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human GLOD4 aa 250 to C-terminus.

View Alternative Names

C17orf25, CGI-150, My027, GLOD4, Glyoxalase domain-containing protein 4

2 Images
Immunoprecipitation - Anti-GLOD4 antibody (AB242089)
  • IP

Supplier Data

Immunoprecipitation - Anti-GLOD4 antibody (AB242089)

GLOD4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242089 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab242089 at 1/1000 dilution.

Lane 1 : ab242089 IP in HEK-293T whole cell lysate.

Lane 2 : ab242089 IP in HEK-293T whole cell lysate

Lane 3 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-GLOD4 antibody (ab242089)

Predicted band size: 35 kDa

false

Western blot - Anti-GLOD4 antibody (AB242089)
  • WB

Supplier Data

Western blot - Anti-GLOD4 antibody (AB242089)

All lanes:

Western blot - Anti-GLOD4 antibody (ab242089) at 1/1000 dilution

Lane 1:

HEK293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 35 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human GLOD4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HC38

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "20-40 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to GLOD4 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLOD4 also known as Glyoxalase Domain Containing 4 is a protein with a significant role in cellular processes. It has a molecular mass of approximately 18 kDa. This protein expresses mainly in the liver brain and kidneys indicating its involvement in multiple organ systems. GLOD4 contains characteristic glyoxalase domains which facilitate its biochemical actions. Studies suggest that GLOD4 structurally participates in stress response and detoxification pathways due to these domain properties.
Biological function summary

Glyoxalase Domain Containing 4 plays important roles in the detoxification of methylglyoxal a byproduct of glycolysis that can be harmful at high levels. GLOD4 does not function alone; it acts as part of the glyoxalase system which includes other Gly and Gly-related enzymes to efficiently convert toxic metabolites into non-toxic substances. Its enzymatic actions support the maintenance of cellular homeostasis and protection against glycation end-products that are damaging to cellular structures.

Pathways

Glyoxalase Domain Containing 4 operates within key metabolic routes like the glycolytic pathway and the detoxification pathway. It collaborates with enzymes like Glyoxalase I and Glyoxalase II in the aforementioned glyoxalase system to perform its roles. The action within these pathways helps reduce oxidative stress aiding in the prevention of cellular damage. GLOD4’s interaction with these enzymes and pathways highlights its importance in maintaining cellular integrity under physiological and stress conditions.

Elevated Glyoxalase Domain Containing 4 expression links to conditions such as diabetes and neurodegenerative disorders. Changes in GLOD4 levels often correspond with disruptions in methylglyoxal metabolism and oxidative stress common in these diseases. Studies also indicate relationships among GLOD4 insulin signaling and proteins such as Glyoxalase I suggesting a connection to the metabolic disturbances found in diabetes. GLOD4's involvement in detoxification pathways also affects the pathogenesis of neurodegenerative diseases potentially influencing cell survival and neuroprotection mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GLOD4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:3 PubMed34815755

2021

Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang Xie
View all publications
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