Anti-GLP-1 antibody [8G9] (ab26278) is a mouse monoclonal antibody that is used to detect GLP-1 in IHC-P, ELISA. Suitable for Human, Mouse samples.
- Over 20 publications
- Trusted since 2005
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (AB26278), expandable thumbnail](https://content.abcam.com/products/images/glp-1-antibody-8g9-ab26278--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108053.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (AB26278)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (AB26278), expandable thumbnail](https://content.abcam.com/products/images/glp-1-antibody-8g9-ab26278--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112918.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (AB26278)")
![Sandwich ELISA - Anti-GLP-1 antibody [8G9] (AB26278), expandable thumbnail](https://content.abcam.com/products/images/glp-1-antibody-8g9-ab26278--sandwich-elisa-img429024.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-GLP-1 antibody [8G9] (AB26278)")
Publications
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- Journal of clinical biochemistry and nutrition 72:28-382023Development of sandwich ELISAs for detecting glucagon-like peptide-1 secretion from intestinal L-cells and their application in STC-1 cells and mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin Odongo,Ken-Yu Hironao,Yoko Yamashita,Hitoshi AshidaPubMed 36777078
- eLife 11:2022Enteroendocrine cell types that drive food reward and aversion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Bai,Nilla Sivakumar,Shenliang Yu,Sheyda Mesgarzadeh,Tom Ding,Truong Ly,Timothy V Corpuz,James C R Grove,Brooke C Jarvie,Zachary A KnightPubMed 35913117
- Science advances 8:eabn3773202214-3-3-zeta mediates GLP-1 receptor agonist action to alter α cell proglucagon processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarlena M Holter,Daryl J Phuong,Isaac Lee,Mridusmita Saikia,Lisa Weikert,Samantha Fountain,Elizabeth T Anderson,Qin Fu,Sheng Zhang,Kyle W Sloop,Bethany P CummingsPubMed 35867787
- Nutrients 14:2022Antidiabetic Effects of pA1c on HFD-Induced Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiriam Cabello-Olmo,María Oneca,María José Pajares,Maddalen Jiménez,Josune Ayo,Ignacio J Encío,Miguel Barajas,Miriam ArañaPubMed 35277051
- Scientific reports 11:238132021Ileal microbial shifts after Roux-en-Y gastric bypass orchestrate changes in glucose metabolism through modulation of bile acids and L-cell adaptation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJerry T Dang,Valentin Mocanu,Heekuk Park,Michael Laffin,Caroline Tran,Naomi Hotte,Shahzeer Karmali,Daniel W Birch,Karen MadsenPubMed 34893681
- International journal of molecular sciences 22:2021Unveiling the Metabolic Effects of Glycomacropeptide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria João Pena,Raquel Costa,Ilda Rodrigues,Sandra Martins,João Tiago Guimarães,Ana Faria,Conceição Calhau,Júlio César Rocha,Nuno BorgesPubMed 34575895
- Genes 12:2021Novel Homozygous Inactivating Mutation in the Gene in an Infant with Congenital Malabsorptive Diarrhea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaetitia Aerts,Nathalie A Terry,Nina N Sainath,Clarivet Torres,Martín G Martín,Bruno Ramos-Molina,John W CreemersPubMed 34068683
- Internal medicine (Tokyo, Japan) 60:1433-14422021The Amount of Residual Incretin Regulates the Pancreatic β-cell Function and Glucose Homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatsuya Kondo,Sayaka Kitano,Nobukazu Miyakawa,Takuro Watanabe,Rieko Goto,Miki Sato,Satoko Hanatani,Masaji Sakaguchi,Motoyuki Igata,Junji Kawashima,Hiroyuki Motoshima,Takeshi Matsumura,Eiichi ArakiPubMed 33952814
- Molecular metabolism 49:1012072021Selective release of gastrointestinal hormones induced by an orally active GPR39 agonist.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaare V Grunddal,Thi A Diep,Natalia Petersen,Iain R Tough,Louise J Skov,Lingzhi Liu,Jesse A Buijink,Franziska Mende,Chunyu Jin,Sara L Jepsen,Louis M E Sørensen,Michael P Achiam,Rune B Strandby,Anders Bach,Bolette Hartmann,Thomas M Frimurer,Siv A Hjorth,Michel Bouvier,Helen Cox,Birgitte HolstPubMed 33711555
- JCI insight 6:2021GLP-1 receptor signaling increases PCSK1 and β cell features in human α cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMridusmita Saikia et. al.PubMed 33554958
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human GCG aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P01275
Reactivity data
Select an application| IHC-P | sELISA | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Synthetic peptide | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Fix in 4% paraformaldehyde in 0.1 phosphate buffer, pH 7.4 overnight at 4°C. |
Species Human | Dilution info 1/2000 | Notes Fix in 4% paraformaldehyde in 0.1 phosphate buffer, pH 7.4 overnight at 4°C. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed:22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Alternative names
Pro-glucagon, GCG
Recommended products
Anti-GLP-1 antibody [8G9] (ab26278) is a mouse monoclonal antibody that is used to detect GLP-1 in IHC-P, ELISA. Suitable for Human, Mouse samples.
- Over 20 publications
- Trusted since 2005
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human GCG aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link P01275
- Clone number
- 8G9
- Purification technique
- Affinity purification Protein A
- Epitope
- C-terminal epitope of GLP-1(7-36)amide
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-GLP-1 antibody [8G9] (ab26278) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), ELISA in Human, Mouse samples.
Trusted by the scientific community
Anti-GLP-1 [8G9] (ab26278) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLP-1 also known as glucagon-like peptide-1 is a 30 or 31 amino acid peptide with a mass of approximately 3.3 kDa. This peptide is mainly secreted by intestinal L-cells and the CNS. It enhances the secretion of insulin in response to glucose making it an important regulator of blood sugar levels. GLP-1 is degraded by dipeptidyl peptidase-4 which rapidly reduces its activity. Researchers often target GLP-1 in studies aimed at understanding metabolic processes and developing therapeutic interventions.
- Biological function summary
GLP-1 influences several physiological functions by acting on its receptor GLP-1R a G-protein coupled receptor. It is not part of a complex but exerts its effects through binding to this receptor. Activation of GLP-1R promotes insulin secretion suppresses glucagon release slows gastric emptying and promotes satiety. This makes GLP-1 an important regulator in energy homeostasis and nutrient absorption. Its effects on appetite and food intake are of particular interest in obesity research.
- Pathways
GLP-1 is an essential component of the incretin pathway which regulates insulin secretion in response to food intake. The interaction between GLP-1 and the GLP-1 receptor activates the adenylate cyclase pathway leading to increased cAMP and PKA signaling in pancreatic beta-cells. This chain of events enhances the insulin-secreting ability of these cells. Furthermore GLP-1 has connections with proteins such as insulin and glucagon through its regulatory functions in glucose metabolism.
- Associated diseases and disorders
GLP-1 is highly relevant to type 2 diabetes and obesity. Its ability to enhance insulin secretion and promote weight loss has made it a target for drug development in the treatment of these conditions. Antidiabetic medications including GLP-1 receptor agonists exploit this mechanism to control blood sugar levels effectively. Additionally GLP-1's connection to insulin makes it a significant focus in diabetes research as imbalances in these proteins contribute to the disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (ab26278), expandable thumbnail](https://content.abcam.com/products/images/glp-1-antibody-8g9-ab26278--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108053.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (ab26278)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (ab26278)
ab26278 (1μg/ml) staining GLP-1 in Human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (ab26278), expandable thumbnail](https://content.abcam.com/products/images/glp-1-antibody-8g9-ab26278--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112918.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (ab26278)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [8G9] (ab26278)
Immunofluorescence analysis of diabetic mice pancreas tissue stained for GLP-1 using ab26278 at 1/500 dilution.
![Sandwich ELISA - Anti-GLP-1 antibody [8G9] (ab26278), expandable thumbnail](https://content.abcam.com/products/images/glp-1-antibody-8g9-ab26278--sandwich-elisa-img429024.jpg "Sandwich ELISA - Anti-GLP-1 antibody [8G9] (ab26278)")
Sandwich ELISA - Anti-GLP-1 antibody [8G9] (ab26278)
Sandwich ELISA graph showing GLP-1 (1-36)amide, GLP-1 (9-36)amide, and GLP-1 (7-36)amide detection using ab26278 as catching antibody and biotinylated Anti-GLP-1 antibody [11E2] ab23468 detection antibody.
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