Rabbit Polyclonal GLP-1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human Pro-glucagon aa 1-50 conjugated to Bovine Serum Albumin.
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- Molecular and cellular biochemistry 478:2779-27872023Individuals with type 2 diabetes have higher density of small intestinal neurotensin-expressing cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilipa P Ferreira,Sofia S Pereira,Madalena M Costa,Marta Guimarães,Nicolai J Wewer Albrechtsen,Jens J Holst,Mário Nora,Mariana P MonteiroPubMed 36920577
- Gut 72:314-32420226α-hydroxylated bile acids mediate TGR5 signalling to improve glucose metabolism upon dietary fiber supplementation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKassem Makki,Harald Brolin,Natalia Petersen,Marcus Henricsson,Dan Ploug Christensen,Muhammad Tanweer Khan,Annika Wahlström,Per-Olof Bergh,Valentina Tremaroli,Kristina Schoonjans,Hanns-Ulrich Marschall,Fredrik BäckhedPubMed 35697422
- Cell reports 38:1104382022BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoep Beumer,Jens Puschhof,Fjodor Yousef Yengej,Lianzheng Zhao,Adriana Martinez-Silgado,Marloes Blotenburg,Harry Begthel,Charelle Boot,Alexander van Oudenaarden,Ye-Guang Chen,Hans CleversPubMed 35235783
- Nutrients 14:2021NSAID-Induced Enteropathy Affects Regulation of Hepatic Glucose Production by Decreasing GLP-1 Secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHussein Herz,Yang Song,Yuanchao Ye,Liping Tian,Benjamin Linden,Marwa Abu El Haija,Yi Chu,Justin L Grobe,Randall W Lengeling,Mohamad MokademPubMed 35010994
- Frontiers in cardiovascular medicine 8:7097412021Decreased Glucagon-Like Peptide-1 Is Associated With Calcific Aortic Valve Disease: GLP-1 Suppresses the Calcification of Aortic Valve Interstitial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Xiao,Qing Zha,Qianru Zhang,Qihong Wu,Zhongli Chen,Ying Yang,Ke Yang,Yan LiuPubMed 34513952
- Frontiers in microbiology 11:5755952020Regulation of Enteroendocrine Cell Networks by the Major Human Gut Symbiont .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmisha Modasia et. al.PubMed 33240233
- Cell reports 32:1080132020Protein O-GlcNAc Modification Links Dietary and Gut Microbial Cues to the Differentiation of Enteroendocrine L Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Zhao,Kaiqun Ren,Xiwen Xiong,Meng Cheng,Zengdi Zhang,Zan Huang,Xiaonan Han,Xiaoyong Yang,Emilyn U Alejandro,Hai-Bin RuanPubMed 32783937
- The Journal of endocrinology 246:223-2362020ACE2 modulates glucose homeostasis through GABA signaling during metabolic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyi Ma,Fei Gao,Qi Chen,Xiuping Xuan,Ying Wang,Hongjun Deng,Fengying Yang,Li YuanPubMed 32698150
- Cell 181:1291-1306.e192020High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoep Beumer et. al.PubMed 32407674
- Frontiers in cellular neuroscience 14:952020Glucagon-Like Peptide-1 Secreting L-Cells Coupled to Sensory Nerves Translate Microbial Signals to the Host Rat Nervous System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria M Buckley,Rebecca O'Brien,Eilish Brosnan,R Paul Ross,Catherine Stanton,Julliette M Buckley,Dervla O'MalleyPubMed 32425756
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human Pro-glucagon aa 1-50 conjugated to Bovine Serum Albumin. The exact immunogen used to generate this antibody is proprietary information. Database link P01275
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mammals | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
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Target data
Function
GlucagonPlays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.Glucagon-like peptide 1Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed:22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable).Glucagon-like peptide 2Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.OxyntomodulinSignificantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.GlicentinMay modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Alternative names
Pro-glucagon, GCG
Recommended products
Rabbit Polyclonal GLP-1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human Pro-glucagon aa 1-50 conjugated to Bovine Serum Albumin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human Pro-glucagon aa 1-50 conjugated to Bovine Serum Albumin. The exact immunogen used to generate this antibody is proprietary information. Database link P01275
- Purification technique
Precipitation Ammonium Sulphate
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLP-1 also known as glucagon-like peptide-1 is a 30 or 31 amino acid peptide with a mass of approximately 3.3 kDa. This peptide is mainly secreted by intestinal L-cells and the CNS. It enhances the secretion of insulin in response to glucose making it an important regulator of blood sugar levels. GLP-1 is degraded by dipeptidyl peptidase-4 which rapidly reduces its activity. Researchers often target GLP-1 in studies aimed at understanding metabolic processes and developing therapeutic interventions.
- Biological function summary
GLP-1 influences several physiological functions by acting on its receptor GLP-1R a G-protein coupled receptor. It is not part of a complex but exerts its effects through binding to this receptor. Activation of GLP-1R promotes insulin secretion suppresses glucagon release slows gastric emptying and promotes satiety. This makes GLP-1 an important regulator in energy homeostasis and nutrient absorption. Its effects on appetite and food intake are of particular interest in obesity research.
- Pathways
GLP-1 is an essential component of the incretin pathway which regulates insulin secretion in response to food intake. The interaction between GLP-1 and the GLP-1 receptor activates the adenylate cyclase pathway leading to increased cAMP and PKA signaling in pancreatic beta-cells. This chain of events enhances the insulin-secreting ability of these cells. Furthermore GLP-1 has connections with proteins such as insulin and glucagon through its regulatory functions in glucose metabolism.
- Associated diseases and disorders
GLP-1 is highly relevant to type 2 diabetes and obesity. Its ability to enhance insulin secretion and promote weight loss has made it a target for drug development in the treatment of these conditions. Antidiabetic medications including GLP-1 receptor agonists exploit this mechanism to control blood sugar levels effectively. Additionally GLP-1's connection to insulin makes it a significant focus in diabetes research as imbalances in these proteins contribute to the disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody (ab22625)
ab22625 (1/2000) staining GLP-1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining in the pancreatic islets.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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