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AB111125

Anti-GLP-1 antibody [EPR4042-407]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • Lab Essentials
  • What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal GLP-1 antibody. Suitable for IHC-P, I-ELISA and reacts with Human, Mouse, Rat, Recombinant full length protein samples. Cited in 5 publications.

View Alternative Names

Pro-glucagon, GCG

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-407] (AB111125)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-407] (AB111125)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labeling GLP-1 with purified ab111125 at 1/10000 dilution (0.11 μg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-407] (AB111125)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-407] (AB111125)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue sections labeling GLP-1 with purified ab111125 at 1/10000 dilution (0.03 μg/mL) for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on α-cells of the islets of Langerhans in rat pancreas.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-407] (AB111125)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1 antibody [EPR4042-407] (AB111125)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling GLP-1 with purified ab111125 at 1/10000 dilution (0.03 μg/mL) for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on α-cells of the islets of Langerhans in mouse pancreas.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Indirect ELISA - Anti-GLP-1 antibody [EPR4042-407] (AB111125)
  • I-ELISA

Unknown

Indirect ELISA - Anti-GLP-1 antibody [EPR4042-407] (AB111125)

ELISA analysis of recombinant GLP-1 proteins using ab111125. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) used as the secondary antibody at 1/2500 dilution (0.24 μg/mL).

GLP1(9-36) is the inactive form.

  • Carrier free

    Anti-GLP-1 antibody [EPR4042-407] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GLP-1 antibody [EPR4042-407]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GLP-1 antibody [EPR4042-407]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GLP-1 antibody [EPR4042-407]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GLP-1 antibody [EPR4042-407]

  • 578 PE

    PE Anti-GLP-1 antibody [EPR4042-407]

  • 660 APC

    APC Anti-GLP-1 antibody [EPR4042-407]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-GLP-1 antibody [EPR4042-407]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4042-407

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

IHC-P, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "62.5 µg/mL", "IELISA-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLP-1 also known as glucagon-like peptide-1 is a 30 or 31 amino acid peptide with a mass of approximately 3.3 kDa. This peptide is mainly secreted by intestinal L-cells and the CNS. It enhances the secretion of insulin in response to glucose making it an important regulator of blood sugar levels. GLP-1 is degraded by dipeptidyl peptidase-4 which rapidly reduces its activity. Researchers often target GLP-1 in studies aimed at understanding metabolic processes and developing therapeutic interventions.
Biological function summary

GLP-1 influences several physiological functions by acting on its receptor GLP-1R a G-protein coupled receptor. It is not part of a complex but exerts its effects through binding to this receptor. Activation of GLP-1R promotes insulin secretion suppresses glucagon release slows gastric emptying and promotes satiety. This makes GLP-1 an important regulator in energy homeostasis and nutrient absorption. Its effects on appetite and food intake are of particular interest in obesity research.

Pathways

GLP-1 is an essential component of the incretin pathway which regulates insulin secretion in response to food intake. The interaction between GLP-1 and the GLP-1 receptor activates the adenylate cyclase pathway leading to increased cAMP and PKA signaling in pancreatic beta-cells. This chain of events enhances the insulin-secreting ability of these cells. Furthermore GLP-1 has connections with proteins such as insulin and glucagon through its regulatory functions in glucose metabolism.

GLP-1 is highly relevant to type 2 diabetes and obesity. Its ability to enhance insulin secretion and promote weight loss has made it a target for drug development in the treatment of these conditions. Antidiabetic medications including GLP-1 receptor agonists exploit this mechanism to control blood sugar levels effectively. Additionally GLP-1's connection to insulin makes it a significant focus in diabetes research as imbalances in these proteins contribute to the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed : 22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable).. Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
See full target information GCG
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 15:1357730 PubMed38595641

2024

Expression of glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide in the rat submandibular gland is influenced by pre- and post-natal high-fat diet exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Pornchanok Sangsuriyothai,Ippei Watari,Saranya Serirukchutarungsee,Sirichom Satrawaha,Katarzyna Anna Podyma-Inoue,Takashi Ono

Heliyon 10:e24340 PubMed38293540

2024

Exploring a novel long-acting glucagon-like peptide-1 receptor agonist built on the albumin-binding domain and XTEN scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhou,Jianhui Li,Guosheng Gao,Yafeng Li,Changzhen Zhang

Pharmaceutical biology 61:737-745 PubMed37129023

2023

Hepatoprotective effect of protocatechuic acid against type 2 diabetes-induced liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kaixia Xu,Guang Lu,Qianjin Feng,Shuangchao Chen,Yonghui Wang

iScience 26:106573 PubMed37138777

2023

LBP1C-2 from maintains skeletal muscle satellite cell pool by interaction with FGFR1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Meng,Zhenyu Lv,Xia Chen,Chuanxin Sun,Can Jin,Kan Ding,Chang Chen

Experimental and therapeutic medicine 20:3669-3678 PubMed32855719

2020

Exendin-4 inhibits atrial arrhythmogenesis in a model of myocardial infarction-induced heart failure via the GLP-1 receptor signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Chen,Shunen Xu,Long Wang,Wei Zhou,Ping Li,Na Deng,Qian Tang,Yongkang Li,Lirong Wu,Jiulin Chen,Wei Li
View all publications
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