Name: Anti-GLP-1R antibody [EPR21819]
SKU: ab218532
Rating: 4 (6 reviews)

Anti-GLP-1R antibody [EPR21819] is a rabbit recombinant monoclonal antibody that is used to detect GLP-1R in IHC-Fr, IHC-P, Western blot. Suitable for Mouse, Rat samples.

- Cited in over 20 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency

Images

Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-Fr	IHC-P
Mouse	Tested	Tested	Tested
Rat	Expected	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Rat species is recommended based on IHC-P/ IHC-Fr results, we do not guarantee WB for rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681)

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000 - 1/2000	Notes (Use at 1:1000 for rat species) Performed heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/1000 - 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information Glucagon-like peptide 1 receptor

Function

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed:9568699). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (By similarity). Plays a role in regulating insulin secretion in response to GLP-1 (PubMed:9568699).

Alternative names

Glucagon-like peptide 1 receptor, GLP-1 receptor, GLP-1-R, GLP-1R, Glp1r

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21819
Purification technique: Affinity purification Protein A
Specificity: Rat species is recommended based on IHC-P / IHC-Fr results, we do not guarantee WB for rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-GLP-1R antibody [EPR21819] (ab218532) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-Fr, IHC-P, WB in mouse, rat samples.
Anti-GLP-1R antibody [EPR21819] (ab218532) specifically detects GLP-1R (UniProt ID: O35659; Molecular weight: 51kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-GLP-1R antibody [EPR21819] (ab218532) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GLP-1R antibody [EPR21819] (ab218532) has been cited over 20 times in peer reviewed journals and is trusted by the scientific community.
Anti-GLP-1R antibody [EPR21819] (ab218532) has 6 independent reviews from customers.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)
4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in mouse pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).
Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532)
GLP-1R Western blot staining using rabbit Anti-GLP-1R antibody
All lanes: Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532) at 1/1000 dilution
Lane 1: Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate boiled. at 20 µg
Lane 2: Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate unboiled. at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 46.66 kDa
Exposure time: 20s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for GLP-1R using ab218532 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Performed heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)
4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for GLP-1R using ab218532 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of mouse pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Performed heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)
Immunohistochemical analysis of paraffin-embedded (A) Pancreas tissue from wild-type C57BL/6JGpt mice and (B) Pancreas tissue from GLP-1R knockout mice tissue labeling GLP-1R with ab218532 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Positive staining on (A) Pancreas tissue from wild-type C57BL/6JGpt mice and no staining on (B) Pancreas tissue from GLP-1R knockout mice.

The section was incubated with ab218532 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and GLP-1R-KO homozygous mice (Strain ID: T006049).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)
Immunohistochemical analysis of paraffin-embedded (A) Heart tissue from wild-type C57BL/6JGpt mice and (B) Heart tissue from GLP-1R knockout mice tissue labeling GLP-1R with ab218532 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Negative control: no staining on (A) Heart tissue from wild-type C57BL/6JGpt mice or on (B) Heart tissue from GLP-1R knockout mice.

The section was incubated with ab218532 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and GLP-1R-KO homozygous mice (Strain ID: T006049).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Anti-GLP-1R antibody [EPR21819]

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