Skip to main content

Anti-GLP-1R antibody [EPR21819] ab218532 is a rabbit monoclonal antibody that is used in GLP-1R western blotting and IHC. Suitable for mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR21819 is the most widely used clone for GLP-1R on the market


Images

Western blot - Anti-GLP-1R antibody [EPR21819] (AB218532), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Mouse
Tested
Tested
Tested
Rat
Expected
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Rat species is recommended based on IHC-P/ IHC-Fr results, we do not guarantee WB for rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681)

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681)

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

(Use at 1:1000 for rat species)

Performed heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

(Use at 1:1000 for rat species)

Performed heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed:9568699). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (By similarity). Plays a role in regulating insulin secretion in response to GLP-1 (PubMed:9568699).

Alternative names

Glucagon-like peptide 1 receptor, GLP-1 receptor, GLP-1-R, GLP-1R, Glp1r

Recommended products

Anti-GLP-1R antibody [EPR21819] ab218532 is a rabbit monoclonal antibody that is used in GLP-1R western blotting and IHC. Suitable for mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR21819 is the most widely used clone for GLP-1R on the market

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21819

Purification technique

Affinity purification Protein A

Specificity

Rat species is recommended based on IHC-P / IHC-Fr results, we do not guarantee WB for rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GLP-1R also known as glucagon-like peptide-1 receptor plays an important role in glucose metabolism. This receptor is a member of the class B family of G protein-coupled receptors (GPCRs) and has a molecular mass of approximately 53 kDa. GLP-1R is expressed in pancreatic beta cells the central nervous system and other peripheral tissues. It binds to GLP-1 a peptide hormone initiating a cascade of intracellular signaling that is essential for its physiological functions.

Biological function summary

GLP-1R serves as an important player in the regulation of insulin secretion. It is not part of a complex but acts as a standalone receptor. When GLP-1 binds to GLP-1R it triggers the activation of adenylyl cyclase increasing cyclic AMP levels and facilitating insulin secretion. This process is glucose-dependent meaning that it enhances insulin release only when blood glucose levels are elevated therefore preventing hypoglycemia.

Pathways

GLP-1R is an integral component of the incretin signaling pathway and the insulin signaling pathway. Through these pathways it influences key metabolic processes. Incretin hormones like GLP-1 improve glucose-dependent insulin secretion involving proteins such as GLP-1R and other incretin-related receptors. Additionally GLP-1R interacts with insulin receptor pathways to modulate glucose uptake in tissues.

Associated diseases and disorders

GLP-1R has significant implications in type 2 diabetes and obesity. Its role in enhancing insulin secretion makes it a target for therapeutic interventions using GLP-1 analogs like liraglutide. Liraglutide structure mimics natural GLP-1 effectively agonizing GLP-1R to achieve desired metabolic outcomes. Additionally liraglutide GLP-1 analog therapies show promise in obesity management by influencing appetite regulation through GLP-1R pathways in the central nervous system.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532), expandable thumbnail

    Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532)

    All lanes: Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532) at 1/1000 dilution

    Lane 1: Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate boiled. at 20 µg

    Lane 2: Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate unboiled. at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 46.66 kDa

    Exposure time: 20s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for GLP-1R using ab218532 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Performed heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)

    4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for GLP-1R using ab218532 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of mouse pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Performed heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (ab218532)

    4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in mouse pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com