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AB218532

Anti-GLP-1R antibody [EPR21819]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

4

(6 Reviews)

|

(31 Publications)

Anti-GLP-1R antibody [EPR21819] (ab218532) is a rabbit monoclonal antibody detecting GLP-1R in Western Blot, IHC-P, IHC-Fr. Suitable for Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

Glucagon-like peptide 1 receptor, GLP-1 receptor, GLP-1-R, GLP-1R, Glp1r

6 Images
Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)

4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in mouse pancreatic islet cells (PMID : 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for GLP-1R using ab218532 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of mouse pancreatic islet cells (PMID : 24467746) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)

4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue stained for GLP-1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in rat pancreatic islet cells (PMID : 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for GLP-1R using ab218532 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in rat pancreatic islet cells (PMID : 24467746) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP-1R antibody [EPR21819] (AB218532)

Immunohistochemical analysis of paraffin-embedded (A) Pancreas tissue from wild-type C57BL/6JGpt mice and (B) Pancreas tissue from GLP-1R knockout mice tissue labeling GLP-1R with ab218532 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) Pancreas tissue from wild-type C57BL/6JGpt mice and no staining on (B) Pancreas tissue from GLP-1R knockout mice.
The section was incubated with ab218532 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and GLP-1R-KO homozygous mice (Strain ID : T006049).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Western blot - Anti-GLP-1R antibody [EPR21819] (AB218532)
  • WB

Lab

Western blot - Anti-GLP-1R antibody [EPR21819] (AB218532)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GLP-1R antibody [EPR21819] (ab218532) at 1/1000 dilution

Lane 1:

Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate boiled. at 20 µg

Lane 2:

Beta-TC-6 (Mouse pancreas insulinoma beta cell) whole cell lysate unboiled. at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 53 kDa

Observed band size: 46.66 kDa

false

Exposure time: 20s

  • Carrier free

    Anti-GLP-1R antibody [EPR21819] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21819

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Rat species is recommended based on IHC-P / IHC-Fr results, we do not guarantee WB for rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Rat species is recommended based on IHC-P/ IHC-Fr results, we do not guarantee WB for rat.</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (<a href='/en-us/products/unavailable/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab94681'>ab94681</a>)</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (<a href='/en-us/products/unavailable/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab94681'>ab94681</a>)</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "<p>(Use at 1:1000 for rat species)</p><p>Performed heat mediated antigen retrieval using <a href='/en-us/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a> (Tris/EDTA buffer, pH 9.0).</p>" } } }
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Product details

Product Specifications
Anti-GLP-1R antibody [EPR21819] (ab218532) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-Fr, IHC-P, WB in mouse, rat samples.
Anti-GLP-1R antibody [EPR21819] (ab218532) specifically detects GLP-1R (UniProt ID: O35659; Molecular weight: 51kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-GLP-1R antibody [EPR21819] (ab218532) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GLP-1R antibody [EPR21819] (ab218532) has been cited over 20 times in peer reviewed journals and is trusted by the scientific community.
Anti-GLP-1R antibody [EPR21819] (ab218532) has 6 independent reviews from customers.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLP-1R also known as glucagon-like peptide-1 receptor plays an important role in glucose metabolism. This receptor is a member of the class B family of G protein-coupled receptors (GPCRs) and has a molecular mass of approximately 53 kDa. GLP-1R is expressed in pancreatic beta cells the central nervous system and other peripheral tissues. It binds to GLP-1 a peptide hormone initiating a cascade of intracellular signaling that is essential for its physiological functions.
Biological function summary

GLP-1R serves as an important player in the regulation of insulin secretion. It is not part of a complex but acts as a standalone receptor. When GLP-1 binds to GLP-1R it triggers the activation of adenylyl cyclase increasing cyclic AMP levels and facilitating insulin secretion. This process is glucose-dependent meaning that it enhances insulin release only when blood glucose levels are elevated therefore preventing hypoglycemia.

Pathways

GLP-1R is an integral component of the incretin signaling pathway and the insulin signaling pathway. Through these pathways it influences key metabolic processes. Incretin hormones like GLP-1 improve glucose-dependent insulin secretion involving proteins such as GLP-1R and other incretin-related receptors. Additionally GLP-1R interacts with insulin receptor pathways to modulate glucose uptake in tissues.

GLP-1R has significant implications in type 2 diabetes and obesity. Its role in enhancing insulin secretion makes it a target for therapeutic interventions using GLP-1 analogs like liraglutide. Liraglutide structure mimics natural GLP-1 effectively agonizing GLP-1R to achieve desired metabolic outcomes. Additionally liraglutide GLP-1 analog therapies show promise in obesity management by influencing appetite regulation through GLP-1R pathways in the central nervous system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed : 9568699). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (By similarity). Plays a role in regulating insulin secretion in response to GLP-1 (PubMed : 9568699).
See full target information Glucagon-like peptide 1 receptor
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 37:1783-1788 PubMed40902582

2025

Reassessment of antibody-based detection of the murine T cell GLP-1 receptor.

Applications

WB, ICC/IF, IHC-P, Flow Cyt

Species

Mouse, Mouse, Mouse, Mouse

Chi Kin Wong,Bernardo Yusta,Jason C L Tong,Johannes Broichhagen,David J Hodson,Daniel J Drucker

International journal of molecular medicine 56: PubMed40709382

2025

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chao Yang,Ya Yue,Yi-Ting Wang,John Y Chiang,Ben-Chung Cheng,Tsuen-Wei Hsu,Yi-Ling Chen,Yi-Chen Li,Hon-Kan Yip

Nature medicine 31:3128-3140 PubMed40691365

2025

Modulation of metabolic, inflammatory and fibrotic pathways by semaglutide in metabolic dysfunction-associated steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Jara,Jenny Norlin,Mette Skalshøi Kjær,Kasper Almholt,Kristian M Bendtsen,Elisabetta Bugianesi,Kenneth Cusi,Elisabeth D Galsgaard,Milan Geybels,Lise L Gluud,Lea M Harder,Rohit Loomba,Gianluca Mazzoni,Philip N Newsome,Louise M Nitze,Mads S Palle,Vlad Ratziu,Anne-Sophie Sejling,Vincent W-S Wong,Quentin M Anstee,Lotte B Knudsen

International journal of molecular sciences 26: PubMed40076675

2025

Neurons Co-Expressing GLP-1, CCK, and PYY Receptors Particularly in Right Nodose Ganglion and Innervating Entire GI Tract in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Laura Lansbury,Vasiliki Vana,Mari Lilith Lund,Mette Q Ludwig,Esmira Mamedova,Laurent Gautron,Myrtha Arnold,Kristoffer Lihme Egerod,Rune Ehrenreich Kuhre,Jens Juul Holst,Jens Rekling,Thue W Schwartz,Stanislava Pankratova,Oksana Dmytriyeva

Scientific reports 14:17867 PubMed39090182

2024

Sodium butyrate improves renal injury in diabetic nephropathy through AMPK/SIRT1/PGC-1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kaili Ye,Yanling Zhao,Wen Huang,Yonglin Zhu

Molecular medicine (Cambridge, Mass.) 30:58 PubMed38720283

2024

Glucagon-like peptide-1 receptor agonist exendin 4 ameliorates diabetes-associated vascular calcification by regulating mitophagy through the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Chen,Hao-Jie Jin,Zi-Heng Wu,Bao-Fu Zhang,Jun Wu,Zi-Yi Huang,Ying-Peng Huang,Xin-Wu Lu,Xiang-Tao Zheng

World journal of methodology 14:88518 PubMed38577198

2024

Rikkunshito increases peripheral incretin-hormone levels in humans and rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Kono,Shinji Furuya,Hidenori Akaike,Katsutoshi Shoda,Yoshihiko Kawaguchi,Hidetake Amemiya,Hiromichi Kawaida,Daisuke Ichikawa

PloS one 18:e0296006 PubMed38117787

2023

High frequency of germline recombination in Nestin-Cre transgenic mice crossed with Glucagon-like peptide 1 receptor floxed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Kajitani,Takashi Miyazawa,Tomoaki Inoue,Nao Kajitani,Masamichi Fujita,Yukina Takeichi,Yasutaka Miyachi,Ryuichi Sakamoto,Yoshihiro Ogawa

Iranian journal of basic medical sciences 26:1423-1430 PubMed37970444

2023

Considering the protective effect of exendin-4 against oxidative stress in spiral ganglion neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxia Wu,Yangxi Ou,Siji Wang,Fenghui Yu,Xiaoxia Fan,Houyong Kang,Tao Chen

Endocrine connections 12: PubMed37681442

2023

Effects of liraglutide on ANP secretion and cardiac dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Shenghe Luo,Yunhui Zuo,Xiaotian Cui,Meiping Zhang,Honghua Jin,Lan Hong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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