Rabbit Polyclonal GLRA2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycine receptor subunit alpha-2 aa 1-200.
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Publications
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- Journal of medical genetics 60:193-2032022gene mutations cause high myopia in humans and mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Tian,Ping Tong,Gong Chen,Meichun Deng,Tian'e Cai,Runyi Tian,Zimin Zhang,Kun Xia,Zhengmao HuPubMed 35396272
- Cellular physiology and biochemistry : internation 45:1084-10962018Transient Receptor Potential Vanilloid 4 Activation-Induced Increase in Glycine-Activated Current in Mouse Hippocampal Pyramidal Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengwen Qi et. al.PubMed 29439248
- The Journal of physiology 595:5285-53002017Presence of ethanol-sensitive glycine receptors in medium spiny neurons in the mouse nucleus accumbens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesB Förstera et. al.PubMed 28524260
- Scientific reports 6:249532016A biofidelic 3D culture model to study the development of brain cellular systems.Applications:ICC/IFReactive species:RatM Ren,C Du,E Herrero Acero,M D Tang-Schomer,N ÖzkucurPubMed 27112667
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Glycine receptor subunit alpha-2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P23416
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info - | Notes - |
Target data
Function
Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:15302677, PubMed:16144831, PubMed:2155780, PubMed:23895467, PubMed:25445488, PubMed:26370147, PubMed:34473954). Channel opening is also triggered by taurine and beta-alanine (PubMed:15302677). Plays a role in synaptic plasticity (By similarity). Contributes to the generation of inhibitory postsynaptic currents, and is involved in the down-regulation of neuronal excitability (PubMed:25445488). Plays a role in cellular responses to ethanol (PubMed:23895467).
Alternative names
Glycine receptor subunit alpha-2, GLRA2
Recommended products
Rabbit Polyclonal GLRA2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Glycine receptor subunit alpha-2 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Glycine receptor subunit alpha-2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P23416
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GLRA2 protein also referred to as Glycine Receptor Alpha 2 is a critical component of the glycine receptor complex. It mainly contributes to inhibitory neurotransmission in the central nervous system. This protein has a molecular mass of approximately 51 kDa and is predominantly expressed in the brain and spinal cord. Being an important part of the pentameric ligand-gated ion channel family GLRA2 plays a substantial role in mediating synaptic signal transmission.
- Biological function summary
The glycine receptor activity of GLRA2 facilitates the inhibitory effects of glycine neurotransmitters in neurons. It forms a ligand-gated chloride channel by assembling with other subunits into pentameric complexes usually associating alongside alpha-1 and beta subunits. This receptor complex regulates chloride ions influx leading to hyperpolarization and decreased neuronal excitability essential for proper nervous system functioning.
- Pathways
GLRA2 participates in the neurotransmitter-gated ion channel signaling pathway which is significant for fast synaptic inhibition in the spinal cord and brainstem regions. This protein associates with gephyrin a cytoplasmic protein that helps maintain receptor localization at synapses. Additionally GLRA2 interacts with other similar receptor subunits in the formation of ion channels important for maintaining synaptic signal regulation.
- Associated diseases and disorders
GLRA2 disruptions have been linked to neurological conditions such as hyperekplexia and epilepsy. Hyperekplexia characterized by an exaggerated startle response is often associated with mutations in this protein leading to impaired inhibitory signaling. The protein’s interaction with other ion channel subunits and related structural proteins like GLRB can contribute to these diseases. Understanding these connections highlights the importance of balanced glycinergic signaling for neurological health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-GLRA2 antibody (ab97628)
10 % SDS PAGE
All lanes: Western blot - Anti-GLRA2 antibody (ab97628) at 1/5000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLRA2 antibody (ab97628)
ab97628, at a 1/500 dilution, staining GLRA2 in paraffin embedded MDAMB468 xenograft by Immunohistochemistry.
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