Anti-GLS2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (AB113509)

Immunohistochemical analysis of paraffin-embedded human liver tissue labelling GLS2 with ab113509 at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• ICC

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Immunocytochemistry - Anti-GLS2 antibody (AB113509)

Immunocytochemical analysis of rat liver cells labelling GLS2 with ab113509 at 20 μg/mL.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (AB113509)

Immunohistochemistry of GLS2 in mouse brain tissue with ab113509 at 20 μg/ml (green), followed by DAPI (blue) and phylloidin (red) staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (AB113509)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GLS2 antibody (ab113509) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-GLS2 antibody (AB113509)

ab113509 IHC-FoFr on rat brain tissue sections. The antibody was used at 1/1000 in the free floating technique for 18 hours at 20°C. There was no blocking step.

Image Courtesy of Sophie Pezet, INSERM, France

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (AB113509)

Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-GLS2 antibody (ab113509) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-GLS2 antibody (AB113509)

All lanes:

Western blot - Anti-GLS2 antibody (ab113509) at 2 µg/mL

All lanes:

Human pancreas cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

false

• WB

Supplier Data

Western blot - Anti-GLS2 antibody (AB113509)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-GLS2 antibody (ab113509) at 1 µg/mL

Lane 1:

Mouse brain tissue lysate at 15 µg

Lane 2:

Rat brain tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 66 kDa

false