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Rabbit Polyclonal GLS2 antibody. Suitable for ICC, WB, IHC-P, IHC-Fr and reacts with Rat, Mouse samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human GLS2.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human GLS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UI32
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBIHC-PIHC-Fr
Mouse
Expected
Expected
Tested
Expected
Rat
Tested
Tested
Expected
Tested

Tested
Tested

Species

Rat

Dilution info

20 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

0.50000-1.00000 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.

Alternative names

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Rabbit Polyclonal GLS2 antibody. Suitable for ICC, WB, IHC-P, IHC-Fr and reacts with Rat, Mouse samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human GLS2.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human GLS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UI32
Purification technique

Affinity purification Immunogen

Specificity

The immunogen used for this product shares 100% homology with GLS1. Cross-reactivity with this protein has not been confirmed experimentally.

WB has been tested in rat only.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

GLS2 impacts important cellular functions due to its ability to enhance the production of glutamate and ammonia from glutamine. This enzyme forms part of the broader metabolic networks and contributes significantly to many cellular activities. It does not function as part of a larger protein complex but interacts with other metabolic enzymes to support energy and biosynthetic demands of the cell.

Activity summary

GLS2 also known as Glutaminase 2 is an enzyme involved in the conversion of glutamine to glutamate. It plays an important role in the regulation of cellular glutamine metabolism. This enzyme has a molecular weight of approximately 66 kDa and is expressed in various tissues including liver brain and kidney. In the mitochondria GLS2 plays an integral part in the cellular metabolic processes.

Pathways

GLS2 influences both the glutaminolysis pathway and the tricarboxylic acid (TCA) cycle. Its activity enables the provision of carbon skeletons for the TCA cycle through the generation of alpha-ketoglutarate from glutamate. This enzyme can interact with proteins such as GDH (Glutamate Dehydrogenase) which further processes the glutamate. These pathways collaboratively sustain bioenergetics and synaptic functions particularly within neurons.

Associated diseases and disorders

GLS2 has links to cancer and neurodegenerative diseases. In cancer abnormal GLS2 expression can affect cellular proliferation through altered glutamine metabolism intersecting with pathways involving proteins like MYC. In neurodegenerative disorders such as Alzheimer's disease GLS2 expression changes can impact neuronal function and survival possibly involving interactions with proteins related to synaptic maintenance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry - Anti-GLS2 antibody (ab113509), expandable thumbnail

    Immunocytochemistry - Anti-GLS2 antibody (ab113509)

    Immunocytochemical analysis of rat liver cells labelling GLS2 with ab113509 at 20 μg/mL.

  • Western blot - Anti-GLS2 antibody (ab113509), expandable thumbnail

    Western blot - Anti-GLS2 antibody (ab113509)

    Lane 1: Western blot - Anti-GLS2 antibody (AB113509) at 0.5 µg/mL

    Lane 2: Western blot - Anti-GLS2 antibody (AB113509) at 1 µg/mL

    All lanes: GLS2 in Rat Kidney Lysate

    Predicted band size: 66 kDa

  • Immunohistochemistry (Frozen sections) - Anti-GLS2 antibody (ab113509), expandable thumbnail
    Image Courtesy of Sophie Pezet, INSERM, France

    Immunohistochemistry (Frozen sections) - Anti-GLS2 antibody (ab113509)

    Ab113509 IHC-FoFr on rat brain tissue sections. The antibody was used at 1/1000 in the free floating technique for 18 hours at 20°C. There was no blocking step.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509)

    Immunohistochemistry of GLS2 in mouse brain tissue with ab113509 at 20 μg/ml (green), followed by DAPI (blue) and phylloidin (red) staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509)

    Immunohistochemical analysis of GLS2 in mouse brain with ab113509 at 5 μg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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