Rabbit Polyclonal GLS2 antibody. Suitable for WB, IHC-P, ICC, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human GLS2.
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- British journal of pharmacology 180:2577-25982023Emodin promotes hepatic stellate cell senescence and alleviates liver fibrosis via a nuclear receptor (Nur77)-mediated epigenetic regulation of glutaminase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Chen,Baoyu Liang,Siwei Xia,Feixia Wang,Zhanghao Li,Jiangjuan Shao,Zili Zhang,Anping Chen,Shizhong Zheng,Feng ZhangPubMed 37263753
- Scientific reports 13:72912023Pancreatic β-cell glutaminase 2 maintains glucose homeostasis under the condition of hyperglycaemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanna Deguchi-Horiuchi,Sawako Suzuki,Eun Young Lee,Takashi Miki,Noriko Yamanaka,Ichiro Manabe,Tomoaki Tanaka,Koutaro YokotePubMed 37147373
- The Journal of clinical investigation 133:2023Asparagine protects pericentral hepatocytes during acute liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Sun,Hadrien Demagny,Adrien Faure,Francesca Pontanari,Antoine Jalil,Nadia Bresciani,Ece Yildiz,Melanie Korbelius,Alessia Perino,Kristina SchoonjansPubMed 36719750
- Cells 12:2022Glutamine Produces Ammonium to Tune Lysosomal pH and Regulate Lysosomal Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Xiong,Thi Thu Trang Luu,Kartik Venkatachalam,Guangwei Du,Michael X ZhuPubMed 36611873
- Cells 11:2022Long-Term Hyperglycemia Causes Depressive Behaviors in Mice with Hypoactive Glutamatergic Activity in the Medial Prefrontal Cortex, Which Is Not Reversed by Insulin Treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Hyeong Baek,Hyeonwi Son,Jae Soon Kang,Dae Young Yoo,Hye Jin Chung,Dong Kun Lee,Hyun Joon KimPubMed 36552776
- Translational oncology 26:1015622022ARNTL2 is an indicator of poor prognosis, promotes epithelial-to-mesenchymal transition and inhibits ferroptosis in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Zhang,Guangyao Shan,Xing Jin,Xiangyang Yu,GuoShu Bi,Mingxiang Feng,Hao Wang,Miao Lin,Cheng Zhan,Qun Wang,Ming LiPubMed 36228410
- Frontiers in neural circuits 16:9562012022Prelimbic cortex responds to male ultrasonic vocalizations in the presence of a male pheromone in female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkari Asaba,Kensaku Nomoto,Takuya Osakada,Tomohiko Matsuo,Ko Kobayakawa,Reiko Kobayakawa,Kazushige Touhara,Kazutaka Mogi,Takefumi KikusuiPubMed 36247727
- Cancers 14:2022Identification and Validation of a Novel Ferroptotic Prognostic Genes-Based Signature of Clear Cell Renal Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyuan Shi,Jianzhong Zheng,Qing Liang,Yankuo Liu,Yi Yang,Rui Wang,Mingshan Wang,Qian Zhang,Zuodong Xuan,Huimin Sun,Kejia Wang,Chen ShaoPubMed 36230613
- Biomedicines 10:2022Molecular Mechanisms Underlying the Retrieval and Extinction of Morphine Withdrawal-Associated Memories in the Basolateral Amygdala and Dentate Gyrus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAurelio Franco-García,Francisco José Fernández-Gómez,Victoria Gómez-Murcia,Juana M Hidalgo,M Victoria Milanés,Cristina NúñezPubMed 35327388
- PloS one 17:e02613702022Curcumin suppresses tumorigenesis by ferroptosis in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuelei Cao,Yao Li,Yongbin Wang,Tao Yu,Chao Zhu,Xuezhi Zhang,Jialiang GuanPubMed 35041678
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GLS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UI32
Reactivity data
Select an application| WB | IHC-P | ICC | IHC-Fr | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected |
| Mouse | Tested | Tested | Expected | Expected |
| Rat | Tested | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Species Mouse | Dilution info 0.5-2 µg/mL | Notes - |
Species Rat | Dilution info 0.5-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Species Mouse | Dilution info 0.5-2 µg/mL | Notes - |
Species Rat | Dilution info 0.5-2 µg/mL | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Alternative names
GA, GLS2, GLS, L-glutaminase, L-glutamine amidohydrolase
Recommended products
Rabbit Polyclonal GLS2 antibody. Suitable for WB, IHC-P, ICC, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human GLS2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GLS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UI32
- Purification technique
- Affinity purification Immunogen
- Specificity
The immunogen used for this product shares 100% homology with GLS1. Cross-reactivity with this protein has not been confirmed experimentally.
WB has been tested in rat only.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLS2 also known as Glutaminase 2 is an enzyme involved in the conversion of glutamine to glutamate. It plays an important role in the regulation of cellular glutamine metabolism. This enzyme has a molecular weight of approximately 66 kDa and is expressed in various tissues including liver brain and kidney. In the mitochondria GLS2 plays an integral part in the cellular metabolic processes.
- Biological function summary
GLS2 impacts important cellular functions due to its ability to enhance the production of glutamate and ammonia from glutamine. This enzyme forms part of the broader metabolic networks and contributes significantly to many cellular activities. It does not function as part of a larger protein complex but interacts with other metabolic enzymes to support energy and biosynthetic demands of the cell.
- Pathways
GLS2 influences both the glutaminolysis pathway and the tricarboxylic acid (TCA) cycle. Its activity enables the provision of carbon skeletons for the TCA cycle through the generation of alpha-ketoglutarate from glutamate. This enzyme can interact with proteins such as GDH (Glutamate Dehydrogenase) which further processes the glutamate. These pathways collaboratively sustain bioenergetics and synaptic functions particularly within neurons.
- Associated diseases and disorders
GLS2 has links to cancer and neurodegenerative diseases. In cancer abnormal GLS2 expression can affect cellular proliferation through altered glutamine metabolism intersecting with pathways involving proteins like MYC. In neurodegenerative disorders such as Alzheimer's disease GLS2 expression changes can impact neuronal function and survival possibly involving interactions with proteins related to synaptic maintenance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Immunocytochemistry - Anti-GLS2 antibody (ab113509)
Immunocytochemical analysis of rat liver cells labelling GLS2 with ab113509 at 20 μg/mL.
Image Courtesy of Sophie Pezet, INSERM, FranceImmunohistochemistry (Frozen sections) - Anti-GLS2 antibody (ab113509)
ab113509 IHC-FoFr on rat brain tissue sections. The antibody was used at 1/1000 in the free floating technique for 18 hours at 20°C. There was no blocking step.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509)
Immunohistochemistry of GLS2 in mouse brain tissue with ab113509 at 20 μg/ml (green), followed by DAPI (blue) and phylloidin (red) staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509)
Immunohistochemical analysis of paraffin-embedded human liver tissue labelling GLS2 with ab113509 at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509)
GLS2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat brain tissue using rabbit Anti-GLS2 antibody
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-GLS2 antibody (ab113509) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-GLS2 antibody (ab113509)
All lanes: Western blot - Anti-GLS2 antibody (ab113509) at 2 µg/mL
All lanes: Human pancreas cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Western blot - Anti-GLS2 antibody (ab113509)
GLS2 Western blot staining using rabbit Anti-GLS2 antibody
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-GLS2 antibody (ab113509) at 1 µg/mL
Lane 1: Mouse brain tissue lysate at 15 µg
Lane 2: Rat brain tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 antibody (ab113509)
GLS2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver tissue using rabbit Anti-GLS2 antibody
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GLS2 antibody (ab113509) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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