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AB327124

Anti-GLS2 Antibody [CL7721]

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Mouse Monoclonal GLS2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GLS2.

View Alternative Names

GA, GLS2, GLS, L-glutaminase, L-glutamine amidohydrolase

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)

Immunohistochemical staining of human liver using ab327124 at 1 : 500–1 : 1000 dilution, showing strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)

Immunohistochemical staining of human pancreas using ab327124 at 1 : 500–1 : 1000 dilution, showing moderate granular cytoplasmic positivity in exocrine glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)

Immunohistochemistry analysis in human liver and skeletal muscle tissues using ab327124 at 1 : 500–1 : 1000 dilution. Corresponding GLS2 RNA-seq data are presented for the same tissues.

Western blot - Anti-GLS2 Antibody [CL7721] (AB327124)
  • WB

Supplier Data

Western blot - Anti-GLS2 Antibody [CL7721] (AB327124)

Western blot analysis in human cell line Daudi using ab327124 at 1 µg/ml.

All lanes:

Western blot - Anti-GLS2 Antibody [CL7721] (ab327124)

All lanes:

Human cell line Daudi

Predicted band size: 66.32 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)

Immunohistochemical staining of human prostate using ab327124 at 1 : 500–1 : 1000 dilution, showing no positivity in glandular cells as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLS2 Antibody [CL7721] (AB327124)

Immunohistochemical staining of human skeletal muscle using ab327124 at 1 : 500–1 : 1000 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL7721

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GLS2.

Q9UI32

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
See full target information GLS2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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