Rabbit Recombinant Monoclonal GLS2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img433340.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305)")
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409765.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305)")
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409764.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305)")
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409763.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305)")
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409762.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (AB308305)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Brian was reported to be GLS2 positive in some publications, but this antibody only detected a 50kDa band instead of GLS2 band in brain. We are unsure of the identity of this 50kDa band. We recommend using a higher sensitive ECL substrate to increase the band intensity. |
Species Rat | Dilution info - | Notes Brian was reported to be GLS2 positive in some publications, but this antibody only detected a 50kDa band instead of GLS2 band in brain. We are unsure of the identity of this 50kDa band. We recommend using a higher sensitive ECL substrate to increase the band intensity. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Alternative names
GA, GLS2, GLS, L-glutaminase, L-glutamine amidohydrolase
Recommended products
Rabbit Recombinant Monoclonal GLS2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27332-67
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLS2 also known as Glutaminase 2 is an enzyme involved in the conversion of glutamine to glutamate. It plays an important role in the regulation of cellular glutamine metabolism. This enzyme has a molecular weight of approximately 66 kDa and is expressed in various tissues including liver brain and kidney. In the mitochondria GLS2 plays an integral part in the cellular metabolic processes.
- Biological function summary
GLS2 impacts important cellular functions due to its ability to enhance the production of glutamate and ammonia from glutamine. This enzyme forms part of the broader metabolic networks and contributes significantly to many cellular activities. It does not function as part of a larger protein complex but interacts with other metabolic enzymes to support energy and biosynthetic demands of the cell.
- Pathways
GLS2 influences both the glutaminolysis pathway and the tricarboxylic acid (TCA) cycle. Its activity enables the provision of carbon skeletons for the TCA cycle through the generation of alpha-ketoglutarate from glutamate. This enzyme can interact with proteins such as GDH (Glutamate Dehydrogenase) which further processes the glutamate. These pathways collaboratively sustain bioenergetics and synaptic functions particularly within neurons.
- Associated diseases and disorders
GLS2 has links to cancer and neurodegenerative diseases. In cancer abnormal GLS2 expression can affect cellular proliferation through altered glutamine metabolism intersecting with pathways involving proteins like MYC. In neurodegenerative disorders such as Alzheimer's disease GLS2 expression changes can impact neuronal function and survival possibly involving interactions with proteins related to synaptic maintenance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img433340.jpg "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)")
Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)
This data was developed using Anti-GLS2 antibody [EPR27332-67] ab308304, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control at 1/1000000 dilution.
Brain was reported to be GLS2 positive in some publications, but this antibody only detected a 50kDa band instead of GLS2 band in brain. We are unsure of the identity of this 50kDa band.
We recommend using a higher sensitive ECL substrate to increase the band intensity.
All lanes: Western blot - Anti-GLS2 antibody [EPR27332-67] (Anti-GLS2 antibody [EPR27332-67] ab308304) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Rat liver tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 180s
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409765.jpg "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)")
Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)
This data was developed using 308304, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST. The blot developed using a high sensitivity ECL substrate.
In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-GLS2 antibody [EPR27332-67] (Anti-GLS2 antibody [EPR27332-67] ab308304) at 1/1000 dilution
All lanes: Rat pancreas tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 180s
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409764.jpg "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)")
Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)
This data was developed using 308304, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Kidney (PMID:31804603 and spleen (PMID:10620514)
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 70 secondsAll lanes: Western blot - Anti-GLS2 antibody [EPR27332-67] (Anti-GLS2 antibody [EPR27332-67] ab308304) at 1/1000 dilution
Lane 1: Rat liver tissue lysate at 20 µg
Lane 2: Rat spleen tissue lysate at 20 µg
Lane 3: Rat kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 70s
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409763.jpg "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)")
Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)
This data was developed using 308304, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen
The blot developed using a high sensitivity ECL substrate.
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-GLS2 antibody [EPR27332-67] (Anti-GLS2 antibody [EPR27332-67] ab308304) at 1/1000 dilution
Lane 1: Mouse pancreas tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 180s
![Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305), expandable thumbnail](https://content.abcam.com/products/images/gls2-antibody-epr27332-67-bsa-and-azide-free-ab308305--western-blot-img409762.jpg "Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)")
Western blot - Anti-GLS2 antibody [EPR27332-67] - BSA and Azide free (ab308305)
This data was developed using 308304, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: kidney (PMID:31804603 and PMID:10620514)
The blot developed using a high sensitivity ECL substrate.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 48 secondsAll lanes: Western blot - Anti-GLS2 antibody [EPR27332-67] (Anti-GLS2 antibody [EPR27332-67] ab308304) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 48s
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