Anti-GLTSCR1 antibody [EPR26561-76]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GLTSCR1 with ab302712 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human pancreas. The section was incubated with ab302712 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GLTSCR1 with ab302712 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human placenta. The section was incubated with ab302712 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 (human embryonic kidney epithelial cell) cells labelling GLTSCR1 with ab302712 at 1/100 (4.91 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in HEK-293 cell lineLow expression : U-87 MG (PMID : 29374058) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell, Left) / HEK-293 (human embryonic kidney epithelial cell, Right) cells labelling GLTSCR1 with ab302712 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression : U-87 MG (PMID : 29374058).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GLTSCR1 with ab302712 at 1/500 (0.982 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebrum. The section was incubated with ab302712 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling GLTSCR1 with ab302712 at 1/500 (0.982 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse pancreas. The section was incubated with ab302712 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling GLTSCR1 with ab302712 at 1/100 (4.91 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Immunohistochemical analysis of paraffin-embedded Mouse head and neck tissue labeling GLTSCR1 with ab302712 at 1/500 (0.982 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse head and neck squamous cell carcinoma. The section was incubated with ab302712 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling GLTSCR1 with ab302712 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lane 2 and 3 of this blot were developed using a high sensitivity ECL substrate. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 3 minutes

All lanes:

Western blot - Anti-GLTSCR1 antibody [EPR26561-76] (ab302712) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell), whole cell lysate 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 200 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-GLTSCR1 antibody [EPR26561-76] (AB302712)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The membrane was incubateded with primary antibodies overnight at 4 °C Low expression : U-87 MG (PMID : 29374058) The expression profile observed is consistent with what has been described in the literature (PMID : 29374058). Exposure time : 114 seconds

All lanes:

Western blot - Anti-GLTSCR1 antibody [EPR26561-76] (ab302712) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate 20 µg

Lane 2:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 200 kDa

false

Exposure time: 114s