AB115322

Anti-GluA4 antibody

Name: Anti-GluA4 antibody (ab115322) | Abcam
Brand: Abcam Limited
SKU: AB115322
Price: 590 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal GluA4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human GRIA4 aa 250-300.

View Alternative Names

GluA4, GLUR4, GRIA4, Glutamate receptor 4, GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluA4 antibody (AB115322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluA4 antibody (AB115322)

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115322 at 5 µg/ml, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-GluA4 antibody (AB115322)

All lanes:

Western blot - Anti-GluA4 antibody (ab115322) at 0.3 µg/mL

All lanes:

Human Cerebellum lysate in RIPA buffer at 35 µg

Predicted band size: 101 kDa

Observed band size: 90 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GRIA4 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P48058

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GluA4 also known as GluR4 or AMPA receptor subunit 4 plays a critical role in synaptic transmission. This receptor belongs to the family of ionotropic glutamate receptors which are activated by the neurotransmitter glutamate. As part of the AMPA receptor complex GluA4 contributes to the fast synaptic responses in the central nervous system. The GluA4 protein has a molecular mass of approximately 100 kDa and is prominently expressed in the brain particularly in the cerebellum and cortex although expression can vary by specific neuronal populations.

Biological function summary

GluA4 functions as an integral component of the AMPA receptor complex which mediates synaptic plasticity and strength. AMPA receptors like GluA4 facilitate the influx of sodium and calcium ions upon glutamate binding essential for long-term potentiation a cellular process playing an important role in learning and memory. GluA4 forms tetrameric complexes that co-assemble with other AMPA receptor subunits such as GluA1 GluA2 and GluA3 providing diversity in the receptor properties.

Pathways

GluA4 is embedded within the glutamatergic signaling pathways important for synaptic transmission and plasticity. It plays a part in the long-term potentiation (LTP) and long-term depression (LTD) pathways both of which involve calcium-dependent signaling cascades. These pathways often overlap with the functions of other proteins such as NMDA receptors which also bind glutamate and help regulate the same synaptic responses.

GluA4 has associations with neurological conditions like epilepsy and schizophrenia. Its dysregulation in AMPA receptor-mediated pathways may contribute to aberrant synaptic transmission observed in these disorders. GluA4 works in conjunction with other receptor subunits and related proteins like PSD-95 which further connects it to the synaptic architecture and signaling alterations seen in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (By similarity). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed : 21172611).

See full target information GRIA4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 19:e3001350 PubMed34748545

2021

Adult medial habenula neurons require GDNF receptor GFRα1 for synaptic stability and function.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Fernández-Suárez,Favio A Krapacher,Katarzyna Pietrajtis,Annika Andersson,Lilian Kisiswa,Alvaro Carrier-Ruiz,Marco A Diana,Carlos F Ibáñez

Neuroendocrinology 112:493-509 PubMed34348318

2021

N-Cadherin Regulates GluA1-Mediated Depressive-Like Behavior in Adolescent Female Rat Offspring following Prenatal Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Shuya Shao,Dan Yao,Senya Li,Jing Li,Yufang Si,Huiping Zhang,Zhongliang Zhu,Dongli Song,Hui Li

Frontiers in molecular neuroscience 12:43 PubMed30842726

2019

An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Eli J Rogers,Reem Jada,Kinneret Schragenheim-Rozales,Megha Sah,Marisol Cortes,Matthew Florence,Nina S Levy,Rachel Moss,Randall S Walikonis,Raz Palty,Reut Shalgi,Daniela Lichtman,Alexandra Kavushansky,Nashaat Z Gerges,Itamar Kahn,George K E Umanah,Andrew P Levy

Nature neuroscience 20:219-229 PubMed28067903

2017

Modulation of excitation on parvalbumin interneurons by neuroligin-3 regulates the hippocampal network.

Applications

Unspecified application

Species

Unspecified reactive species

Jai S Polepalli,Hemmings Wu,Debanjan Goswami,Casey H Halpern,Thomas C Südhof,Robert C Malenka

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-GluA4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GluA4 antibody (AB115322)

Western blot - Anti-GluA4 antibody (AB115322)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise