AB109431

Anti-GluA4 antibody [EPR2512(2)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal GluA4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 4 publications.

View Alternative Names

GluA4, GLUR4, GRIA4, Glutamate receptor 4, GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D

3 Images

Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (AB109431)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (AB109431)

Intracellular flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-GluA4 antibody [EPR2512(2)] (AB109431)

All lanes:

Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Human cerebellum lysate at 10 µg

Lane 4:

SH-SY5Y lysate at 10 µg

Predicted band size: 101 kDa

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Lab

Western blot - Anti-GluA4 antibody [EPR2512(2)] (AB109431)

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

Blocking/Diluting Buffer and concentration :
5% NFDM/TBST

All lanes:

Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

Lane 1:

Rat brain prepared in RIPA lysis method at 15 µg

Lane 2:

Rat brain prepared in 1%SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 101 kDa

Observed band size: 101 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2512(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/1000 - 1/10000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GluA4 also known as GluR4 or AMPA receptor subunit 4 plays a critical role in synaptic transmission. This receptor belongs to the family of ionotropic glutamate receptors which are activated by the neurotransmitter glutamate. As part of the AMPA receptor complex GluA4 contributes to the fast synaptic responses in the central nervous system. The GluA4 protein has a molecular mass of approximately 100 kDa and is prominently expressed in the brain particularly in the cerebellum and cortex although expression can vary by specific neuronal populations.

Biological function summary

GluA4 functions as an integral component of the AMPA receptor complex which mediates synaptic plasticity and strength. AMPA receptors like GluA4 facilitate the influx of sodium and calcium ions upon glutamate binding essential for long-term potentiation a cellular process playing an important role in learning and memory. GluA4 forms tetrameric complexes that co-assemble with other AMPA receptor subunits such as GluA1 GluA2 and GluA3 providing diversity in the receptor properties.

Pathways

GluA4 is embedded within the glutamatergic signaling pathways important for synaptic transmission and plasticity. It plays a part in the long-term potentiation (LTP) and long-term depression (LTD) pathways both of which involve calcium-dependent signaling cascades. These pathways often overlap with the functions of other proteins such as NMDA receptors which also bind glutamate and help regulate the same synaptic responses.

GluA4 has associations with neurological conditions like epilepsy and schizophrenia. Its dysregulation in AMPA receptor-mediated pathways may contribute to aberrant synaptic transmission observed in these disorders. GluA4 works in conjunction with other receptor subunits and related proteins like PSD-95 which further connects it to the synaptic architecture and signaling alterations seen in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (By similarity). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed : 21172611).

See full target information GRIA4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 18:1635852 PubMed40771360

2025

Chronic reduction of synaptic proteins in the epileptogenic lesion of patients with hippocampal sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Akiko Oota-Ishigaki,Nami Suzuki,Keiya Iijima,Yutaro Takayama,Yuiko Kimura,Kotaro Hattori,Masaki Iwasaki,Takashi Hayashi

Frontiers in behavioral neuroscience 14:583395 PubMed33328919

2020

Effect of Aggressive Experience in Female Syrian Hamsters on Glutamate Receptor Expression in the Nucleus Accumbens.

Applications

Unspecified application

Species

Unspecified reactive species

Johnathan M Borland,Ellen Kim,Samuel P Swanson,Patrick E Rothwell,Paul G Mermelstein,Robert L Meisel

Nature methods 15:909-912 PubMed30377371

2018

A high-throughput pipeline for validation of antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Sikorski,Adi Mehta,Marit Inngjerdingen,Flourina Thakor,Simon Kling,Tomas Kalina,Tuula A Nyman,Maria Ekman Stensland,Wei Zhou,Gustavo A de Souza,Lars Holden,Jan Stuchly,Markus Templin,Fridtjof Lund-Johansen

Acta physiologica (Oxford, England) 225:e13211 PubMed30347138

2018

The novel antidepressant ketamine enhances dentate gyrus proliferation with no effects on synaptic plasticity or hippocampal function in depressive-like rats.

Applications

Unspecified application

Species

Unspecified reactive species

Henrik Michaëlsson,Mats Andersson,Johan Svensson,Lars Karlsson,Johan Ehn,Georgia Culley,Anders Engström,Nicklas Bergström,Parthenia Savvidi,Hans-Georg Kuhn,Eric Hanse,Henrik Seth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GluA4 antibody [EPR2512(2)]

Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (AB109431)

Western blot - Anti-GluA4 antibody [EPR2512(2)] (AB109431)

Western blot - Anti-GluA4 antibody [EPR2512(2)] (AB109431)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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