Rabbit Polyclonal GluA4 phospho S862 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GRIA4 phospho S862.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GRIA4 phospho S862. The exact immunogen used to generate this antibody is proprietary information. Database link P48058
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (By similarity). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611).
Alternative names
GluA4, GLUR4, GRIA4, Glutamate receptor 4, GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D
Recommended products
Rabbit Polyclonal GluA4 phospho S862 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GRIA4 phospho S862.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GRIA4 phospho S862. The exact immunogen used to generate this antibody is proprietary information. Database link P48058
- Purification technique
- Affinity purification Immunogen
- Specificity
Detects target only when phosphorylated at serine 862.
- Concentration
- Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein GluA4 also known as GluR4 or AMPA receptor subunit 4 plays a critical role in synaptic transmission. This receptor belongs to the family of ionotropic glutamate receptors which are activated by the neurotransmitter glutamate. As part of the AMPA receptor complex GluA4 contributes to the fast synaptic responses in the central nervous system. The GluA4 protein has a molecular mass of approximately 100 kDa and is prominently expressed in the brain particularly in the cerebellum and cortex although expression can vary by specific neuronal populations.
- Biological function summary
GluA4 functions as an integral component of the AMPA receptor complex which mediates synaptic plasticity and strength. AMPA receptors like GluA4 facilitate the influx of sodium and calcium ions upon glutamate binding essential for long-term potentiation a cellular process playing an important role in learning and memory. GluA4 forms tetrameric complexes that co-assemble with other AMPA receptor subunits such as GluA1 GluA2 and GluA3 providing diversity in the receptor properties.
- Pathways
GluA4 is embedded within the glutamatergic signaling pathways important for synaptic transmission and plasticity. It plays a part in the long-term potentiation (LTP) and long-term depression (LTD) pathways both of which involve calcium-dependent signaling cascades. These pathways often overlap with the functions of other proteins such as NMDA receptors which also bind glutamate and help regulate the same synaptic responses.
- Associated diseases and disorders
GluA4 has associations with neurological conditions like epilepsy and schizophrenia. Its dysregulation in AMPA receptor-mediated pathways may contribute to aberrant synaptic transmission observed in these disorders. GluA4 works in conjunction with other receptor subunits and related proteins like PSD-95 which further connects it to the synaptic architecture and signaling alterations seen in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-GluA4 (phospho S862) antibody (ab61054)
All lanes: Western blot - Anti-GluA4 (phospho S862) antibody (ab61054) at 1/500 dilution
Lane 1: extracts from HepG2 cells treated with Forskolin (40nM, 30mins)
Lane 2: extracts from HepG2 cells treated with Forskolin (40nM, 30mins) with immunizing phosphopeptide
Predicted band size: 101 kDa
Observed band size: 102 kDa
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