Anti-Glucagon antibody [EP3070] is a rabbit recombinant monoclonal antibody that is used to detect Glucagon in IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 80 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115110.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98714.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517)")
![Western blot - Anti-Glucagon antibody [EP3070] (AB92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--western-blot-img97845.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucagon antibody [EP3070] (AB92517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40587.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (AB92517)")
![Western blot - Anti-Glucagon antibody [EP3070] (AB92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--western-blot-img7721.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucagon antibody [EP3070] (AB92517)")
Publications
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- The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 28:31-382024Lithium and exercise ameliorate insulin-deficient hyperglycemia by independently attenuating pancreatic α-cell mass and hepatic gluconeogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu-Ryun Jung,Ji-Hye Lee,Hanguk Ryu,Yurong Gao,Jaemin LeePubMed 38154962
- The Journal of endocrinology 259:2023Sex-based impact of pancreatic islet stressors in GluCreERT2/Rosa26-eYFP mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeil Tanday,Aimee Coulter-Parkhill,R Charlotte Moffett,Karthick Suruli,Vaibhav Dubey,Peter R Flatt,Nigel IrwinPubMed 37650517
- Molecular medicine reports 28:2023Vascular endothelial growth factor B regulates insulin secretion in β cells of type 2 diabetes mellitus mice via PLCγ and the IP3R‑evoked Ca2/CaMK2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuqi Li,Rongrong Li,Xu Luo,Fang Xu,Meizi Yang,Lanhui Zheng,Qihao Wu,Wenguo Jiang,Yana LiPubMed 37681454
- Forensic science, medicine, and pathology 20:149-1652023Immunohistochemical expression of HMGB1 and related proteins in the skin as a possible tool for determining post-mortem interval: a preclinical study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabio De-Giorgio,Eva Bergamin,Alfonso Baldi,Roberto Gatta,Vincenzo L PascaliPubMed 37490201
- EMBO reports 24:e562272023Hypoxia causes pancreatic β-cell dysfunction and impairs insulin secretion by activating the transcriptional repressor BHLHE40.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomonori Tsuyama,Yoshifumi Sato,Tatsuya Yoshizawa,Takaaki Matsuoka,Kazuya YamagataPubMed 37341148
- iScience 26:1067482023Pancreas-derived DPP4 is not essential for glucose homeostasis under metabolic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvgenia Fadzeyeva,Cassandra A A Locatelli,Natasha A Trzaskalski,My-Anh Nguyen,Megan E Capozzi,Branka Vulesevic,Nadya M Morrow,Peyman Ghorbani,Antonio A Hanson,Ilka Lorenzen-Schmidt,Mary-Anne Doyle,Richard Seymour,Elodie M Varin,Morgan D Fullerton,Jonathan E Campbell,Erin E MulvihillPubMed 37216093
- Frontiers in immunology 13:10596872023Metabolic effects of CCL5 deficiency in lean and obese mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Zhou,Xiyan Liao,Qin Zeng,Haowei Zhang,Jianfeng Song,Wanyu Hu,Xiaoxiao Sun,Yujin Ding,Dandan Wang,Yalun Xiao,Tuo DengPubMed 36713454
- International journal of molecular sciences 24:2023Identification of FGF13 as a Potential Biomarker and Target for Diagnosis of Impaired Glucose Tolerance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Chen,Fangyu Li,Yuanyuan Gao,Fengying Yang,Li YuanPubMed 36675322
- International journal of molecular sciences 24:2023Effect of Sirolimus/Metformin Co-Treatment on Hyperglycemia and Cellular Respiration in BALB/c Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlia Albawardi,Dhanya Saraswathiamma,Charu Sharma,Abdulghani Elomami,Abdul-Kader Souid,Saeeda AlmarzooqiPubMed 36674739
- Molecular metabolism 67:1016502022Selective ablation of P53 in pancreatic beta cells fails to ameliorate glucose metabolism in genetic, dietary and pharmacological models of diabetes mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCelina Uhlemeyer,Nadine Müller,Michael Rieck,Jennifer Kuboth,Caroline Schlegel,Kerstin Grieß,Tim Florian Dorweiler,Sonja Heiduschka,Jürgen Eckel,Michael Roden,Eckhard Lammert,Markus Stoffel,Bengt-Frederik BelgardtPubMed 36470401
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/8000 | Notes For unpurified use at 1/1000 - 1/5000. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/8000 | Notes For unpurified use at 1/1000 - 1/5000. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/8000 | Notes For unpurified use at 1/1000 - 1/5000. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.3 µg/mL | Notes - |
Species Rat | Dilution info 0.3 µg/mL | Notes - |
Species Human | Dilution info 0.3 µg/mL | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed:22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Alternative names
Pro-glucagon, GCG
Recommended products
Anti-Glucagon antibody [EP3070] is a rabbit recombinant monoclonal antibody that is used to detect Glucagon in IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 80 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP3070
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Glucagon antibody [EP3070] (ab92517) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in human, mouse, rat samples.
Anti-Glucagon antibody [EP3070] (ab92517) specifically detects Glucagon (UniProt ID: P01275; Molecular weight: 8kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Glucagon antibody [EP3070] (ab92517) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Glucagon antibody [EP3070] (ab92517) has been cited over 81 times in peer reviewed journals and is trusted by the scientific community.
Anti-Glucagon antibody [EP3070] (ab92517) has 8 independent reviews from customers.
Related Products
Antibody clone EP3070 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 555, Biotin (Alexa Fluor® 647 Anti-Glucagon antibody [EP3070] ab307337, Alexa Fluor® 488 Anti-Glucagon antibody [EP3070] ab307340, Alexa Fluor® 594 Anti-Glucagon antibody [EP3070] ab311840, Alexa Fluor® 555 Anti-Glucagon antibody [EP3070] ab313316, Biotin Anti-Glucagon antibody [EP3070] ab314399).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115110.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98714.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
![Western blot - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--western-blot-img97845.jpg "Western blot - Anti-Glucagon antibody [EP3070] (ab92517)")
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
Blocking and diluting buffer: 5% NFDM/TBST.
The bands around 75 kDa are non-specific signals.
All lanes: Western blot - Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/mL
All lanes: Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40587.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
Unpurified ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded Human pancreas tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--western-blot-img7721.jpg "Western blot - Anti-Glucagon antibody [EP3070] (ab92517)")
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
Blocking and diluting buffer: 5% NFDM/TBST.
The bands around 75 kDa are non-specific signals.
All lanes: Western blot - Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/mL
All lanes: Mouse pancreas lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73585.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling Glucagon with Purified ab92517 at 1:8000 dilution (0.038 µg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0)Goat Anti-Rabbit IgG H&L (HRP) ab97051was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
![Western blot - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--western-blot-img97599.jpg "Western blot - Anti-Glucagon antibody [EP3070] (ab92517)")
Western blot - Anti-Glucagon antibody [EP3070] (ab92517)
Blocking and diluting buffer: 5% NFDM/TBST.
The bands around 75 kDa are non-specific signals.
All lanes: Western blot - Anti-Glucagon antibody [EP3070] (ab92517) at 0.3 µg/mL
All lanes: Rat pancreas lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73585.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] (ab92517)")
![Western blot - Anti-Glucagon antibody [EP3070] (ab92517), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-ep3070-ab92517--western-blot-img97599.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucagon antibody [EP3070] (ab92517)")
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