Anti-Glucagon antibody [EP3070] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078)

This data was developed using ab92517, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded human pancreas labelling Glucagon with ab92517 at a concentration of 0.02µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC2) 100°C, pH8.5 for 32mins.

ab92517 anti-Glucagon antibody [EP3070] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078)

Unpurified ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded human pancreas tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 μg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 μg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue sections labeling Glucagon with purified ab92517 at 1/8000 dilution (0.038 μg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92517).

• WB

Lab

Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078)

The bands around 75 kDa are non-specific signals.

All lanes:

Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/1000 dilution

All lanes:

Capan-1 ( Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

false

• WB

Lab

Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078)

The bands around 75 kDa are non-specific signals.

All lanes:

Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/5000 dilution

Lane 1:

Mouse pancreas lysates at 15 µg

Lane 2:

Rat pancreas lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

false