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Rabbit Recombinant Monoclonal GLP-1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078), expandable thumbnail
  • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078), expandable thumbnail
  • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (AB167078), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Please refer to the original abID, Anti-Glucagon antibody [EP3070] ab92517, for information on recommended dilutions.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Please refer to the original abID, Anti-Glucagon antibody [EP3070] ab92517, for information on recommended dilutions.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Please refer to the original abID, Anti-Glucagon antibody [EP3070] ab92517, for information on recommended dilutions.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Please refer to the original abID, Anti-Glucagon antibody [EP3070] ab92517, for information on recommended dilutions.

Species
Rat
Dilution info
-
Notes

Please refer to the original abID, Anti-Glucagon antibody [EP3070] ab92517, for information on recommended dilutions.

Species
Human
Dilution info
-
Notes

Please refer to the original abID, Anti-Glucagon antibody [EP3070] ab92517, for information on recommended dilutions.

Associated Products

Select an associated product type

8 products for Alternative Product

5 products for Alternative Version

Target data

Function

Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed:22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

Additional Targets

Glucagon

Alternative names

Pro-glucagon, GCG

Recommended products

Rabbit Recombinant Monoclonal GLP-1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP3070
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab167078 is the carrier-free version of Anti-Glucagon antibody [EP3070] ab92517.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Glucagon also known as the “hunger hormone" is a 29-amino acid peptide hormone with a mass of approximately 3.5 kDa. It plays an important role in glucose metabolism and regulation of blood sugar levels. The human pancreas specifically the alpha cells located in the islets of Langerhans synthesizes and secretes glucagon. This hormone binds to glucagon receptors which are widely expressed across tissues including the liver and kidney where it initiates a cascade of signaling events.

Biological function summary

Glucagon plays a central role in maintaining glucose homeostasis. It increases blood glucose by promoting gluconeogenesis and glycogenolysis in the liver. Though glucagon mainly acts independently it exhibits significant interactions with other metabolic hormones such as insulin. This interaction helps balance blood sugar levels as glucagon and insulin work in opposition to ensure optimal blood glucose regulation.

Pathways

Glucagon primarily impacts the cAMP signaling pathway significantly increasing intracellular cAMP levels. This pathway initiates a complex series of downstream events including increased enzyme activity for gluconeogenesis and glycogen breakdown in the liver. Glucagon also cross-talks with insulin signaling pathways enabling the intertwined regulation of metabolism through these hormones and maintaining glucose balance.

Associated diseases and disorders

Glucagon is closely linked to diabetes and hyperglycemia. Individuals with diabetes often exhibit dysregulated glucagon secretion leading to unstable blood sugar levels. Glucagon interacts with insulin in the pathology of diabetes where an improper balance between these hormones can exacerbate the disease. The understanding of glucagon's role in these conditions makes it a target for new therapeutic strategies including recombinant glucagon treatments and assays.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labeling Glucagon with purified Anti-Glucagon antibody [EP3070] ab92517 at 1/8000 dilution (0.038 μg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucagon antibody [EP3070] ab92517).

  • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078), expandable thumbnail

    Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

    The bands around 75 kDa are non-specific signals.

    All lanes: Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/1000 dilution

    All lanes: Capan-1 ( Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

    Unpurified Anti-Glucagon antibody [EP3070] ab92517, at 1/1000 dilution, staining Glucagon in paraffin embedded human pancreas tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucagon antibody [EP3070] ab92517).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling Glucagon with purified Anti-Glucagon antibody [EP3070] ab92517 at 1/8000 dilution (0.038 μg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucagon antibody [EP3070] ab92517).

  • Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078), expandable thumbnail

    Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

    The bands around 75 kDa are non-specific signals.

    All lanes: Western blot - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078) at 1/5000 dilution

    Lane 1: Mouse pancreas lysates at 15 µg

    Lane 2: Rat pancreas lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [EP3070] - BSA and Azide free (ab167078)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue sections labeling Glucagon with purified Anti-Glucagon antibody [EP3070] ab92517 at 1/8000 dilution (0.038 μg/ml). Heat mediated antigen retrieval was performed using using citrate (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucagon antibody [EP3070] ab92517).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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