Anti-Glucagon antibody [K79bB10] ab10988 is a mouse monoclonal antibody that is used in Glucagon IHC. Suitable for and rat samples.
- Antibody clone K79bB10 is the most widely used clone for Glucagon on the market
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (AB10988), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-k79bb10-ab10988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46553.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (AB10988)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (AB10988), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-k79bb10-ab10988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76986.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (AB10988)")
Publications
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- Frontiers in neuroscience 17:11792762023The effects of targeted vagus nerve stimulation on glucose homeostasis in STZ-induced diabetic rodents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElliott W Dirr,Yogi Patel,Richard D Johnson,Kevin J OttoPubMed 37397461
- Nature communications 14:33952023Transparent tissue in solid state for solvent-free and antifade 3D imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFu-Ting Hsiao,Hung-Jen Chien,Ya-Hsien Chou,Shih-Jung Peng,Mei-Hsin Chung,Tzu-Hui Huang,Li-Wen Lo,Chia-Ning Shen,Hsiu-Pi Chang,Chih-Yuan Lee,Chien-Chia Chen,Yung-Ming Jeng,Yu-Wen Tien,Shiue-Cheng TangPubMed 37296117
- Frontiers in endocrinology 14:11610852023Role of Delta/Notch-like EGF-related receptor in blood glucose homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNelmari Ruiz-Otero,Rejji KuruvillaPubMed 37223028
- Journal of diabetes 15:409-4182023β-Cell glucokinase expression was increased in type 2 diabetes subjects with better glycemic control.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwen Liu,Hui Fu,Fuyun Kang,Guang Ning,Qicheng Ni,Weiqing Wang,Qidi WangPubMed 36942376
- Nature communications 14:10202023THADA inhibition in mice protects against type 2 diabetes mellitus by improving pancreatic β-cell function and preserving β-cell mass.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuqing Zhang,Shan Han,Congcong Liu,Yuanwen Zheng,Hao Li,Fei Gao,Yuehong Bian,Xin Liu,Hongbin Liu,Shourui Hu,Yuxuan Li,Zi-Jiang Chen,Shigang Zhao,Han ZhaoPubMed 36823211
- Nature communications 14:6002023Cryo-EM structure supports a role of AQP7 as a junction protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Huang,Raminta Venskutonytė,Rashmi B Prasad,Hamidreza Ardalani,Sofia W de Maré,Xiao Fan,Ping Li,Peter Spégel,Nieng Yan,Pontus Gourdon,Isabella Artner,Karin Lindkvist-PeterssonPubMed 36737436
- Nature communications 14:2352023Hyperaminoacidemia induces pancreatic α cell proliferation via synergism between the mTORC1 and CaSR-Gq signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulong Gong,Bingyuan Yang,Dingdong Zhang,Yue Zhang,Zihan Tang,Liu Yang,Katie C Coate,Linlin Yin,Brittney A Covington,Ravi S Patel,Walter A Siv,Katelyn Sellick,Matthew Shou,Wenhan Chang,E Danielle Dean,Alvin C Powers,Wenbiao ChenPubMed 36646689
- Molecular metabolism 67:1016592022Inhibition of stearoyl-CoA desaturase 1 in the mouse impairs pancreatic islet morphogenesis and promotes loss of β-cell identity and α-cell expansion in the mature pancreas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAneta M Dobosz,Justyna Janikiewicz,Ewelina Krogulec,Anna Dziewulska,Anna Ajduk,Marcin Szpila,Hanna Nieznańska,Andrzej A Szczepankiewicz,Dorota Wypych,Agnieszka DobrzynPubMed 36529318
- EBioMedicine 87:1043792022A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFarooq Syed,Divya Singhal,Koen Raedschelders,Preethi Krishnan,Robert N Bone,Madeline R McLaughlin,Jennifer E Van Eyk,Raghavendra G Mirmira,Mei-Ling Yang,Mark J Mamula,Huanmei Wu,Xiaowen Liu,Carmella Evans-MolinaPubMed 36463755
- Xenotransplantation 30:e127872022Advantages of the retroperitoneal retrocolic space as the transplant site for encapsulated xenogeneic islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Geng,Qi Zhang,Ting Li,Ting Huang,Hailian Wang,Qiao Zhou,Shaoping Deng,Yanshuang Zhao,Yanjiao Li,Chunming Cheng,Carmen Gonelle-Gispert,Leo H Buhler,Yi WangPubMed 36454040
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01274
Abcam Recommends
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Predicted |
| Rat | Tested |
| Cat | Predicted |
| Common marmoset | Predicted |
| Dog | Predicted |
| Guinea pig | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Syrian hamster | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Guinea pig, Cat, Dog, Human, Pig, Common marmoset, Syrian hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Glucagon. Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1. Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis. Glucagon-like peptide 2. Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Oxyntomodulin. Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. Glicentin. May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Additional Targets
Glucagon
Alternative names
Pro-glucagon, GCG
Recommended products
Anti-Glucagon antibody [K79bB10] ab10988 is a mouse monoclonal antibody that is used in Glucagon IHC. Suitable for and rat samples.
- Antibody clone K79bB10 is the most widely used clone for Glucagon on the market
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P01274
- Clone number
- K79bB10
- Purification technique
- Proprietary technique
- Concentration
- Purification notes
Purified from Tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340. Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glucagon also known as the “hunger hormone" is a 29-amino acid peptide hormone with a mass of approximately 3.5 kDa. It plays an important role in glucose metabolism and regulation of blood sugar levels. The human pancreas specifically the alpha cells located in the islets of Langerhans synthesizes and secretes glucagon. This hormone binds to glucagon receptors which are widely expressed across tissues including the liver and kidney where it initiates a cascade of signaling events.
- Biological function summary
Glucagon plays a central role in maintaining glucose homeostasis. It increases blood glucose by promoting gluconeogenesis and glycogenolysis in the liver. Though glucagon mainly acts independently it exhibits significant interactions with other metabolic hormones such as insulin. This interaction helps balance blood sugar levels as glucagon and insulin work in opposition to ensure optimal blood glucose regulation.
- Pathways
Glucagon primarily impacts the cAMP signaling pathway significantly increasing intracellular cAMP levels. This pathway initiates a complex series of downstream events including increased enzyme activity for gluconeogenesis and glycogen breakdown in the liver. Glucagon also cross-talks with insulin signaling pathways enabling the intertwined regulation of metabolism through these hormones and maintaining glucose balance.
- Associated diseases and disorders
Glucagon is closely linked to diabetes and hyperglycemia. Individuals with diabetes often exhibit dysregulated glucagon secretion leading to unstable blood sugar levels. Glucagon interacts with insulin in the pathology of diabetes where an improper balance between these hormones can exacerbate the disease. The understanding of glucagon's role in these conditions makes it a target for new therapeutic strategies including recombinant glucagon treatments and assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-k79bb10-ab10988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46553.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)")
Image courtesy of Thomas Schoeppe by customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)
ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988), expandable thumbnail](https://content.abcam.com/products/images/glucagon-antibody-k79bb10-ab10988--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76986.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucagon antibody [K79bB10] (ab10988)
Formali-fixed, paraffin-embedded rat pancreas tissue stained for Glucagon using ab10988 at 10 μg/ml in immunohistochemical analysis.
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