AB75240

Anti-Glucagon Receptor antibody

Name: Anti-Glucagon Receptor antibody (ab75240) | Abcam
Brand: Abcam Limited
SKU: AB75240
Price: 450 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(9 Publications)

Anti-Glucagon Receptor antibody (ab75240) is a rabbit polyclonal antibody detecting Glucagon Receptor in Western Blot, ICC/IF. Suitable for Human.

- Trusted since 2009

View Alternative Names

Glucagon receptor, GL-R, GCGR

2 Images

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Western blot - Anti-Glucagon Receptor antibody (AB75240)

All lanes:

Western blot - Anti-Glucagon Receptor antibody (ab75240) at 1/500 dilution

Lane 1:

extracts from COLO205 cells at 15 µg

Lane 2:

extracts from COLO205 cells at 15 µg with immunizing peptide

Predicted band size: 54 kDa

Observed band size: 54 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Glucagon Receptor antibody (AB75240)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glucagon Receptor antibody (AB75240)

Left panel : immunofluorescence analysis of HeLa cells with 1/500 ab75240. Right panel : same, after pre-incubation with the immunizing peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human Glucagon receptor. The exact immunogen used to generate this antibody is proprietary information.

P47871

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Glucagon Receptor antibody (ab75240) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Glucagon Receptor?
Anti-Glucagon Receptor (ab75240) specifically detects a band for Glucagon Receptor (UniProt: P47871) at a molecular weight of 54kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glucagon receptor also known as GCGR is a protein located on cell membranes and is about 62 kDa in mass. This receptor belongs to the G protein-coupled receptor (GPCR) family which plays a role in transmitting signals from glucagon—a hormone involved in glucose metabolism. GCGR is expressed mainly in liver kidney and pancreatic cells as well as some regions of the brain. Scientists often use recombinant glucagon proteins to study receptor behaviors and interactions due to their specific binding properties.

Biological function summary

Glucagon receptor activation primarily mediates glucose homeostasis by stimulating hepatic glucose production. It facilitates glycogenolysis and gluconeogenesis processes that increase blood glucose levels especially when glucose availability is low. The receptor does not operate in a complex but interacts closely with secondary messengers like cAMP (cyclic adenosine monophosphate) to trigger downstream signaling cascades. This action allows cells to respond quickly to changing hormonal signals in the bloodstream.

Pathways

Glucagon receptor is an important component of the glucagon signaling pathway and plays a part in the insulin resistance pathway. Upon activation by glucagon the receptor stimulates adenylate cyclase increasing cAMP production which activates protein kinase A (PKA). This process is closely linked with the insulin receptor pathway where both influence glucose and lipid metabolism balancing the metabolic responses within the liver and other tissues.

Glucagon receptor involvement is significant in diabetes and metabolic syndrome. Dysfunctional GCGR activity can contribute to hyperglycemia in type 2 diabetes by failing to regulate glucose production properly. Additionally it links to lipid metabolism disorders affecting triglyceride levels. Researchers focus on the receptor's relationship with insulin and other hormones to develop therapeutic strategies targeting glucagon and its receptor to manage these conditions effectively. Anti-glucagon drugs are under investigation to better control glucose levels in diabetic patients.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed : 32193322, PubMed : 38346960). Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.

See full target information Glucagon receptor

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 30: PubMed40649254

2025

Glucagon and Glucose Availability Influence Metabolic Heterogeneity and Malignancy in Pancreatic Neuroendocrine Tumour (pNET) Cells: Novel Routes for Therapeutic Targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Ferreira,Isabel Lemos,Cindy Mendes,Beatriz Chumbinho,Fernanda Silva,Daniela Pereira,Emanuel Vigia,Luís G Gonçalves,António Figueiredo,Daniela Cavaco,Jacinta Serpa

Journal of the Endocrine Society 9:bvaf056 PubMed40271226

2025

The Glucagon Receptor Is Expressed in the Frontal Cortex and Impaired Signaling Associates With Cognitive Decline.

Applications

Unspecified application

Species

Unspecified reactive species

Sasha A S Kjeldsen,Jonas Folke,Maud E Ottenheijm,Marie Winther-Sørensen,Jónvá Hentze,Pia Nyeng,Sara L Garcia,Agata Casado-Sainz,Sanne Simone Kaalund,Reidar Albrechtsen,Karina Banasik,Søren Brunak,Nina L Hansen,Jens Juul Holst,Mette M Rosenkilde,Jørgen Rungby,Majken K Jensen,Birgitte Georg,Jens Hannibal,Tomasz Brudek,Susana Aznar,Nicolai J Wewer Albrechtsen

Diabetologia 67:528-546 PubMed38127123

2023

GLP-1 metabolite GLP-1(9-36) is a systemic inhibitor of mouse and human pancreatic islet glucagon secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Nikhil R Gandasi,Rui Gao,Lakshmi Kothegala,Abigail Pearce,Cristiano Santos,Samuel Acreman,Davide Basco,Anna Benrick,Margarita V Chibalina,Anne Clark,Claudia Guida,Matthew Harris,Paul R V Johnson,Jakob G Knudsen,Jinfang Ma,Caroline Miranda,Makoto Shigeto,Andrei I Tarasov,Ho Yan Yeung,Bernard Thorens,Ingrid W Asterholm,Quan Zhang,Reshma Ramracheya,Graham Ladds,Patrik Rorsman

The Journal of biological chemistry 299:105045 PubMed37451484

2023

An insulin-regulated arrestin domain protein controls hepatic glucagon action.

Applications

Unspecified application

Species

Unspecified reactive species

Sezin Dagdeviren,Megan F Hoang,Mohsen Sarikhani,Vanessa Meier,Jake C Benoit,Marinna C Okawa,Veronika Y Melnik,Elisabeth M Ricci-Blair,Natalie Foot,Randall H Friedline,Xiaodi Hu,Lauren A Tauer,Arvind Srinivasan,Maxim B Prigozhin,Sudha K Shenoy,Sharad Kumar,Jason K Kim,Richard T Lee

Cells 12: PubMed37048171

2023

Glucagon Promotes Gluconeogenesis through the GCGR/PKA/CREB/PGC-1α Pathway in Hepatocytes of the Japanese Flounder .

Applications

Unspecified application

Species

Unspecified reactive species

Mengxi Yang,Mingzhu Pan,Dong Huang,Jiahuan Liu,Yanlin Guo,Yue Liu,Wenbing Zhang

iScience 26:105967 PubMed36824278

2023

High glucose-induced glucagon resistance and membrane distribution of GCGR revealed by super-resolution imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Gao,Hongru Li,Haijiao Xu,Yong Liu,Mingjun Cai,Yan Shi,Jingrui Zhang,Hongda Wang

Immunity, inflammation and disease 10:e3593 PubMed35349748

2022

Potential of PKM2 as a drug target in mouse models with type 1 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Junbin Liu,Zhixia Li,Gan Huang,Zhiguang Zhou,Peilin Zheng

Molecular metabolism 4:961-70 PubMed26909312

2015

Hypothalamic CaMKKβ mediates glucagon anorectic effect and its diet-induced resistance.

Applications

Species

Unspecified reactive species

Mar Quiñones,Omar Al-Massadi,Rosalía Gallego,Johan Fernø,Carlos Diéguez,Miguel López,Ruben Nogueiras

Molecular systems biology 8:596 PubMed22864381

2012

The glucose-deprivation network counteracts lapatinib-induced toxicity in resistant ErbB2-positive breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kakajan Komurov,Jen-Te Tseng,Melissa Muller,Elena G Seviour,Tyler J Moss,Lifeng Yang,Deepak Nagrath,Prahlad T Ram

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucagon Receptor antibody

Western blot - Anti-Glucagon Receptor antibody (AB75240)

Immunocytochemistry/ Immunofluorescence - Anti-Glucagon Receptor antibody (AB75240)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise