Anti-Glucagon Receptor antibody [EPR29792-511]

• WB

Supplier Data

Western blot - Anti-Glucagon Receptor antibody [EPR29792-511] (AB323750)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : breast and eyeball.

Lane 4 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 180 seconds, Lane 4 : 103 secodns

All lanes:

Western blot - Anti-Glucagon Receptor antibody [EPR29792-511] (ab323750) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 80 µg

Lane 2:

Human breast tissue lysate at 80 µg

Lane 3:

Human eyeball tissue lysate at 80 µg

Lane 4:

Human liver cancer tissue lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 75 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Glucagon Receptor antibody [EPR29792-511] (AB323750)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

GCGR is a glycoprotein of approximately 75 kDa and detected as a 52kDa band after treated with Protein Deglycosylation MIX II.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Glucagon Receptor antibody [EPR29792-511] (ab323750) at 1/1000 dilution

Lane 1:

Untreated mouse liver tissue lysate at 30 µg

Lane 2:

Mouse liver tissue lysate treated with Protein Deglycosylation MIX II at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,52 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Glucagon Receptor antibody [EPR29792-511] (AB323750)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain, spleen, breast, colon and eyeball.

The identity of the lower MW band at approximately 25 kDa (in lanes 9-10) is unknown.

Samples are non-boiled as boiling may cause protein aggregation.

To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-7 : 48 seconds, Lanes 8-10 : 180 seconds

All lanes:

Western blot - Anti-Glucagon Receptor antibody [EPR29792-511] (ab323750) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 80 µg

Lane 5:

Mouse breast tissue lysate at 80 µg

Lane 6:

Mouse colon tissue lysate at 80 µg

Lane 7:

Mouse eyeball tissue lysate at 80 µg

Lane 8:

Rat liver tissue lysate at 80 µg

Lane 9:

Rat breast tissue lysate at 80 µg

Lane 10:

Rat colon tissue lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false