Anti-Glucagon Receptor antibody [RP23040219]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucagon Receptor antibody [RP23040219] (AB313488)

Immunofluorescent analysis of HepG2 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous Glucagon Receptor/GCGR with ab313488 at 5µg/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of Glucagon Receptor/GCGR protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating Membrane localisation of Glucagon Receptor/GCGR. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-Glucagon Receptor Antibody [RP23040219] (AB313488)

All lanes:

Western blot - Anti-Glucagon Receptor Antibody [RP23040219] (ab313488) at 2.5 µg/mL

Lane 1:

U87MG cell lysate at 30 µg

Lane 2:

HepG2 cell lysate at 30 µg

Lane 3:

COLO205 cell lysate at 30 µg

Lane 4:

HeLa cell lysate at 30 µg

Lane 5:

HEK293 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 50 kDa

true