AB307606

Anti-Glucocorticoid Receptor antibody [2153CT307.32.80] - BSA and Azide free

Recombinant
KO Validated
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Knockout Tested Mouse Recombinant Monoclonal Glucocorticoid Receptor antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1

2 Images

Supplier Data

Western blot - Anti-Glucocorticoid Receptor antibody [2153CT307.32.80] - BSA and Azide free (AB307606)

This data was produced using ab307605, the same antibody clone but in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Glucocorticoid Receptor antibody [2153CT307.32.80] (<a href='/en-us/products/primary-antibodies/glucocorticoid-receptor-antibody-2153ct3073280-ab307605'>ab307605</a>) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 85 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-Glucocorticoid Receptor antibody [2153CT307.32.80] - BSA and Azide free (AB307606)

This data was produced using ab307605, the same antibody clone but in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab307605 was shown to bind specifically to Glucocorticoid Receptor. A band was observed at 85 kDa in wild-type HeLa cell lysates with whereas no signal observed at this size in Glucocorticoid Receptor knockout cell line ab261766 (knockout cell lysate ab257009).

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Lane 1:

Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human NR3C1 (Glucocorticoid Receptor) knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-nr3c1-glucocorticoid-receptor-knockout-hela-cell-lysate-ab257009'>ab257009</a>) at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Observed band size: 85 kDa

true

Exposure time: 136s

Unconjugated

Anti-Glucocorticoid Receptor antibody [2153CT307.32.80]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2153CT307.32.80

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.

Biological function summary

The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.

Pathways

The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.

Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for glucocorticoids (GC) (PubMed : 27120390, PubMed : 37478846). Has a dual mode of action : as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed : 28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed : 9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed : 25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).. Isoform Alpha. Has transcriptional activation and repression activity (PubMed : 11435610, PubMed : 15769988, PubMed : 15866175, PubMed : 17635946, PubMed : 19141540, PubMed : 19248771, PubMed : 20484466, PubMed : 21664385, PubMed : 23820903). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127). Promotes accurate chromosome segregation during mitosis (PubMed : 25847991). May act as a tumor suppressor (PubMed : 25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).. Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed : 20484466, PubMed : 7769088, PubMed : 8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed : 19248771, PubMed : 26711253). Loses this transcription modulator function on its own (PubMed : 20484466). Has no hormone-binding activity (PubMed : 8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed : 26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed : 26711253).. Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed : 20484466).. Isoform GR-P. Increases activity of isoform Alpha.. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity.. Isoform 10. Has transcriptional activation activity.. Isoform Alpha-C1. Has transcriptional activation activity.. Isoform Alpha-C2. Has transcriptional activation activity.. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127, PubMed : 23820903).. Isoform Alpha-D1. Has transcriptional activation activity.. Isoform Alpha-D2. Has transcriptional activation activity.. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127).

See full target information NR3C1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucocorticoid Receptor antibody [2153CT307.32.80] - BSA and Azide free

Western blot - Anti-Glucocorticoid Receptor antibody [2153CT307.32.80] - BSA and Azide free (AB307606)

Western blot - Anti-Glucocorticoid Receptor antibody [2153CT307.32.80] - BSA and Azide free (AB307606)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise