Knockout Tested Mouse Recombinant Monoclonal Glucocorticoid Receptor antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--western-blot-img404501.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678)")
![Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--western-blot-img404500.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678)")
![Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--immunocytochemistry-immunofluorescence-img404443.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403992.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403991.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (AB302678)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IP | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Isoform Alpha. Has transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253). Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466). Isoform GR-P. Increases activity of isoform Alpha. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity. Isoform 10. Has transcriptional activation activity. Isoform Alpha-C1. Has transcriptional activation activity. Isoform Alpha-C2. Has transcriptional activation activity. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903). Isoform Alpha-D1. Has transcriptional activation activity. Isoform Alpha-D2. Has transcriptional activation activity. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1
Recommended products
Knockout Tested Mouse Recombinant Monoclonal Glucocorticoid Receptor antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 41-Glucocorticoid Receptor
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab302678 is a carrier version of Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
- Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
- Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
- Associated diseases and disorders
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--western-blot-img404501.jpg "Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)")
Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)
This data was developed using Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The blot of lane 2 was developed using a high sensitivity ECL substrate.
Exposure time: lane 1: 46 seconds; Lane 2: 37 seconds.All lanes: Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] (Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 94 kDa
![Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--western-blot-img404500.jpg "Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)")
Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)
This data was developed using Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lysates at 20 µg per lane.
False colour image of Western blot: Anti- Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] (Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677 staining at 1/1000 dilution, shown in green; Rabbit anti-GAPDH antibody 16891 loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677 was shown to bind specifically to NR3C1. A band was observed at 94 kDa in wild-type HeLa cell lysates with no signal observed at this size in the NR3C1 knockout cell line. To generate this image, wild-type and NR3C1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a PVDF-FL membrane. Membranes were blocked in Odyssey diluted in equal volume of 0.1 % TBS before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution.
Performed under reducing conditions.All lanes: Western blot - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] (Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677) at 1/1000 dilution
All lanes: H9 (human lymphoma T lymphocyte), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 94 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--immunocytochemistry-immunofluorescence-img404443.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)")
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)
This data was developed using Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Glucocorticoid receptor with Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677 at 1/50 dilution (17.98 µg/mL), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing all the signal translocated from cytoplasm into nucleus in HeLa cells treated with dexamethasone (1 µM) for 20 min.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272) was used to counterstain tubulin at 1/50 dilution (10 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403992.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)
This data was developed using Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Glucocorticoid receptor with Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677 at 1/500 dilution (1.798 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on human cervical cancer is observed.
The section was incubated with Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-41-glucocorticoid-receptor-bsa-and-azide-free-ab302678--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403991.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] - BSA and Azide Free (ab302678)
This data was developed using Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling Glucocorticoid receptor with Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677 at 1/500 dilution (1.798 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on human prostatic hyperplasia is observed.
The section was incubated with Anti-Glucocorticoid receptor antibody [41/Glucocorticoid Receptor] ab302677 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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