Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for IHC-P, IP, WB, ChIP-seq and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 150-200.
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- Nature communications 13:71712022A non-coding GWAS variant impacts anthracycline-induced cardiotoxic phenotypes in human iPSC-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Wu,Fei Shen,Guanglong Jiang,Gloria Xue,Santosh Philips,Laura Gardner,Geneva Cunningham,Casey Bales,Erica Cantor,Bryan Paul SchneiderPubMed 36418322
- Nature communications 13:52532022Multimodal single cell sequencing implicates chromatin accessibility and genetic background in diabetic kidney disease progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParker C Wilson,Yoshiharu Muto,Haojia Wu,Anil Karihaloo,Sushrut S Waikar,Benjamin D HumphreysPubMed 36068241
- American journal of obstetrics and gynecology 226:564.e1-564.e142021Betamethasone phosphate reduces the efficacy of antenatal steroid therapy and is associated with lower birthweights when administered to pregnant sheep in combination with betamethasone acetate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsukasa Takahashi,Erin L Fee,Yuki Takahashi,Masatoshi Saito,Nobuo Yaegashi,Haruo Usuda,Lucy Furfaro,Sean Carter,Augusto F Schmidt,John P Newnham,Alan H Jobe,Matthew W KempPubMed 34626553
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human NR3C1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
Reactivity data
Select an application| IHC-P | IP | WB | ChIP-seq | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Sheep | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep | Dilution info - | Notes - |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg chromatin for 30 µg chromatin | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).Isoform AlphaHas transcriptional activation and repression activity (PubMed:15866175, PubMed:19248771, PubMed:20484466, PubMed:23820903, PubMed:11435610, PubMed:15769988, PubMed:17635946, PubMed:19141540, PubMed:21664385). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).Isoform BetaActs as a dominant negative inhibitor of isoform Alpha (PubMed:7769088, PubMed:8621628, PubMed:20484466). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253).Isoform Alpha-2Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466).Isoform GR-PIncreases activity of isoform Alpha.Isoform Alpha-BMore effective than isoform Alpha in transcriptional activation, but not repression activity.Isoform 10Has transcriptional activation activity.Isoform Alpha-C1Has transcriptional activation activity.Isoform Alpha-C2Has transcriptional activation activity.Isoform Alpha-C3Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903).Isoform Alpha-D1Has transcriptional activation activity.Isoform Alpha-D2Has transcriptional activation activity.Isoform Alpha-D3Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL
Recommended products
Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for IHC-P, IP, WB, ChIP-seq and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 150-200.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human NR3C1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab225886 was affinity purified using an epitope specific to Glucocorticoid Receptor immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
- Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
- Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
- Associated diseases and disorders
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-Glucocorticoid Receptor antibody (ab225886)
Glucocorticoid Receptor was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225886 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225886 at 0.4 μg/ml.
Lane 1: ab225886 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
All lanes: Immunoprecipitation - Anti-Glucocorticoid Receptor antibody (ab225886)
Predicted band size: 85 kDa
Western blot - Anti-Glucocorticoid Receptor antibody (ab225886)
All lanes: Western blot - Anti-Glucocorticoid Receptor antibody (ab225886) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 85 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab225886)
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Glucocorticoid Receptor using ab225886 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
ChIP-sequencing - Anti-Glucocorticoid Receptor antibody (ab225886)
Chromatin from prednisolone treated THP-1 (human monocytic leukemia cell line) cells was immunoprecipitated with ab225886 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Glucocorticoid Receptor binding within a 250 Kb region of chromosome 6 as detected using ab225886.
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