Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 300-400.
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- Animals : an open access journal from MDPI 13:2023Effects of Exogenous Glucocorticoid Infusion on Appetitic Center Development in Postnatal Dairy Bull Calves.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeelee J McCarty,Scott L Pratt,Nathan M LongPubMed 37370490
- Frontiers in pharmacology 13:9712432022Effects of Tibetan medicine metacinnabar (β-HgS) combined with imipramine or sertraline on depression-like symptoms in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYajun Qiao,Cen Li,Ming Zhang,Xingfang Zhang,Lixin Wei,Keshen Cao,Xiaoyuan Zhang,Hongtao Bi,Tingting GaoPubMed 36120298
- Reproductive sciences (Thousand Oaks, Calif.) 29:1001-10192021The Plasminogen Activator System, Glucocorticoid, and Mineralocorticoid Receptors in the Primate Endometrium During Artificial Menstrual Cycles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsra Demirel,Reem Sabouni,Neelima Chandra,Ov D Slayden,David F ArcherPubMed 34796470
- Current issues in molecular biology 43:1669-16842021Effects of Radix Polygalae on Cognitive Decline and Depression in Estradiol Depletion Mouse Model of Menopause.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaeul Han,Junhyuk Choi,Seung-Yun Cha,Byung Il Kim,Hee Kyung Kho,Maeng-Jin Jang,Mi Ae Kim,Sungho Maeng,Heeok HongPubMed 34698102
- Frontiers in pharmacology 12:7137152021The Dual Dose-Dependent Effects of Corticosterone on Hippocampal Cell Apoptosis After Traumatic Brain Injury Depend on the Activation Ratio of Mineralocorticoid Receptors to Glucocorticoid Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Zhang,Mengshi Yang,Qiongyu Yan,Xiaojian Xu,Fei Niu,Jinqian Dong,Yuan Zhuang,Shenghua Lu,Qianqian Ge,Baiyun LiuPubMed 34381366
- Journal of neuroinflammation 17:3182020Corticosteroid receptor rebalancing alleviates critical illness-related corticosteroid insufficiency after traumatic brain injury by promoting paraventricular nuclear cell survival via Akt/CREB/BDNF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Zhang et. al.PubMed 33100225
- Journal of neurochemistry 158:358-3722020Bag-1 mediates glucocorticoid receptor trafficking to mitochondria after corticosterone stimulation: Potential role in regulating affective resilience.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaolei Luo,Yangyang Hou,Yaping Zhang,Lei Feng,Richard G Hunter,Peixiong Yuan,Yue Jia,Haoran Li,Gang Wang,Husseini K Manji,Bruce S McEwen,Chunjie Xiao,Hongkun Bao,Jing DuPubMed 33025573
- Annals of translational medicine 8:9422020Effect of combined chronic predictable and unpredictable stress on depression-like symptoms in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYajun Qiao,Jiubo Zhao,Cen Li,Ming Zhang,Lixin Wei,Xiaoyuan Zhang,Olga Kurskaya,Hongtao Bi,Tingting GaoPubMed 32953742
- Molecular medicine reports 22:2451-24592020ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Wang,Hua Wang,Jianming Chen,Fubin Wang,Shuaiyi Wang,Qiang Zhou,Jichong Ying,Shanzhao Huang,Pu Wang,Fangfang YuanPubMed 32705192
- Brain research bulletin 162:49-582020Gut microbiota modulates stress-induced hypertension through the HPA axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Wu et. al.PubMed 32535221
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NR3C1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
Consider this alternative
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Guinea pig | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 | Notes - |
Species Human | Dilution info 1/20 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Isoform Alpha. Has transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253). Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466). Isoform GR-P. Increases activity of isoform Alpha. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity. Isoform 10. Has transcriptional activation activity. Isoform Alpha-C1. Has transcriptional activation activity. Isoform Alpha-C2. Has transcriptional activation activity. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903). Isoform Alpha-D1. Has transcriptional activation activity. Isoform Alpha-D2. Has transcriptional activation activity. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1
Recommended products
Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 300-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NR3C1 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
- Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
- Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
- Associated diseases and disorders
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
Immunocytochemistry/Immunofluorescence analysis of NIH-3T3 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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