AB3579

Anti-Glucocorticoid Receptor antibody

Name: Anti-Glucocorticoid Receptor antibody (ab3579) | Abcam
Brand: Abcam Limited
SKU: AB3579
Price: 565 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(30 Publications)

Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 200-300.

View Alternative Names

GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3579)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3579)

Immunofluorescent analysis of ab3579 shows staining in HeLa (Human epithelial adenocarcinoma cell line) cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab3579at a dilution of 1/100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3579)

Immunofluorescence analysis of Glucocorticoid Receptor in MDA-MB-231 cells (serum-starved) and MDA-MB-231 cells serum-starved for 24 hours, followed by 1 μM Dexamethasone treatment for 2 hours using ab3579. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 2% BSA. The cells were labeled with ab3579 at 1/100 dilution in 0.1% BSA, incubated at 4°C overnight followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor®488 at 1/2000 dilution (Panel a : Green) in MDA-MB-231 treated cells. Nuclei (Panel b : Blue) were stained DAPI. F-actin (Panel c : Red) was stained with Rhodamine Phalloidin 1/300. (Panel d) represents the merged image showing nuclear localization of NR3C1 protein in MDA-MB-231 treated cells. (Panel e) represents the merged image of MDA-MB-231 untreated cells, that shows cytoplasmic localization of NR3C1 protein. (Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3579)

Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

Supplier Data

Western blot - Anti-Glucocorticoid Receptor antibody (AB3579)

All lanes:

Western blot - Anti-Glucocorticoid Receptor antibody (ab3579) at 1 µg/mL

Lane 1:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg/mL with 5% Milk in TBST

Lane 2:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST

Lane 3:

K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg with 5% Milk in TBST

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST

Lane 5:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg with 5% Milk in TBST

Secondary

All lanes:

HRP conjugate at 1/1000 dilution

Predicted band size: 85 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NR3C1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P04150

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p>Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.

Biological function summary

The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.

Pathways

The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.

Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for glucocorticoids (GC) (PubMed : 27120390, PubMed : 37478846). Has a dual mode of action : as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed : 28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed : 9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed : 25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).. Isoform Alpha. Has transcriptional activation and repression activity (PubMed : 11435610, PubMed : 15769988, PubMed : 15866175, PubMed : 17635946, PubMed : 19141540, PubMed : 19248771, PubMed : 20484466, PubMed : 21664385, PubMed : 23820903). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127). Promotes accurate chromosome segregation during mitosis (PubMed : 25847991). May act as a tumor suppressor (PubMed : 25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).. Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed : 20484466, PubMed : 7769088, PubMed : 8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed : 19248771, PubMed : 26711253). Loses this transcription modulator function on its own (PubMed : 20484466). Has no hormone-binding activity (PubMed : 8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed : 26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed : 26711253).. Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed : 20484466).. Isoform GR-P. Increases activity of isoform Alpha.. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity.. Isoform 10. Has transcriptional activation activity.. Isoform Alpha-C1. Has transcriptional activation activity.. Isoform Alpha-C2. Has transcriptional activation activity.. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127, PubMed : 23820903).. Isoform Alpha-D1. Has transcriptional activation activity.. Isoform Alpha-D2. Has transcriptional activation activity.. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127).

See full target information NR3C1

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 31:2335-2348 PubMed37574723

2023

Distinct response of adipocyte progenitors to glucocorticoids determines visceral obesity via the TEAD1-miR-27b-PRDM16 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Lv,Fan Xia,Jing Yu,Yunlu Sheng,Yi Jin,Yanqiang Li,Guoxian Ding

Nucleic acids research 50:11635-11653 PubMed36399508

2022

Glucocorticoids unmask silent non-coding genetic risk variants for common diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Thanh Thanh L Nguyen,Huanyao Gao,Duan Liu,Trudy Janice Philips,Zhenqing Ye,Jeong-Heon Lee,Geng-Xian Shi,Kaleigh Copenhaver,Lingxin Zhang,Lixuan Wei,Jia Yu,Huan Zhang,Abhijeet Barath,Maggie Luong,Cheng Zhang,Alexandre Gaspar-Maia,Hu Li,Liewei Wang,Tamas Ordog,Richard M Weinshilboum

Translational psychiatry 12:458 PubMed36316324

2022

Two kinds of transcription factors mediate chronic morphine-induced decrease in miR-105 in medial prefrontal cortex of rats.

Applications

Unspecified application

Species

Unspecified reactive species

Junfang Zhang,Xinli Guo,Zhangyin Cai,Yan Pan,Hao Yang,Yali Fu,Zixuan Cao,Yaxian Wen,Chao Lei,Chenshan Chu,Yu Yuan,Dongyang Cui,Pengyu Gao,Bin Lai,Ping Zheng

Scientific reports 11:6506 PubMed33753762

2021

Metabolomics analysis of follicular fluid coupled with oocyte aspiration reveals importance of glucocorticoids in primate periovulatory follicle competency.

Applications

Unspecified application

Species

Unspecified reactive species

Sweta Ravisankar,Carol B Hanna,Kelsey E Brooks,Melinda J Murphy,Nash Redmayne,Junghyun Ryu,Jason M Kinchen,Shawn L Chavez,Jon D Hennebold

The Journal of experimental medicine 218: PubMed33688917

2021

Hepatic Gadd45β promotes hyperglycemia and glucose intolerance through DNA demethylation of PGC-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Yang Jiao,Yao Li,Jingjing Jiang,Lin Zhao,Menghui Li,Bin Li,Zheng Yan,Xuejin Chen,Xiaoying Li,Yan Lu

Science advances 7: PubMed33627425

2021

Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Huayu Zhu,Jun Li,Yize Li,Zhao Zheng,Hao Guan,Hongtao Wang,Ke Tao,Jiaqi Liu,Yunchuan Wang,Wanfu Zhang,Chao Li,Jie Li,Lintao Jia,Wendong Bai,Dahai Hu

The Journal of clinical investigation 130:3791-3804 PubMed32510471

2020

Obesity-induced excess of 17-hydroxyprogesterone promotes hyperglycemia through activation of glucocorticoid receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Lu,E Wang,Ying Chen,Bing Zhou,Jiejie Zhao,Liping Xiang,Yiling Qian,Jingjing Jiang,Lin Zhao,Xuelian Xiong,Zhiqiang Lu,Duojiao Wu,Bin Liu,Jing Yan,Rong Zhang,Huijie Zhang,Cheng Hu,Xiaoying Li

Scientific reports 9:2772 PubMed30809020

2019

Extensive epigenomic integration of the glucocorticoid response in primary human monocytes and in vitro derived macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wang,Luca Nanni,Boris Novakovic,Wout Megchelenbrink,Tatyana Kuznetsova,Hendrik G Stunnenberg,Stefano Ceri,Colin Logie

American journal of physiology. Endocrinology and metabolism 314:E39-E52 PubMed28928236

2017

Histone deacetylase inhibition ameliorates hypertension and hyperglycemia in a model of Cushing's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Hae-Ahm Lee,Seol-Hee Kang,Mina Kim,Eunjo Lee,Hyun-Min Cho,Eun-Kyung Moon,Inkyeom Kim

PLoS medicine 13:e1002200 PubMed27997540

2016

IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study.

Applications

Species

Human

Yunlei Li,Jessica G C A M Buijs-Gladdines,Kirsten Canté-Barrett,Andrew P Stubbs,Eric M Vroegindeweij,Willem K Smits,Ronald van Marion,Winand N M Dinjens,Martin Horstmann,Roland P Kuiper,Rogier C Buijsman,Guido J R Zaman,Peter J van der Spek,Rob Pieters,Jules P P Meijerink

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Glucocorticoid Receptor antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3579)

Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3579)

Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3579)

Western blot - Anti-Glucocorticoid Receptor antibody (AB3579)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

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