Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 200-300.
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- Obesity (Silver Spring, Md.) 31:2335-23482023Distinct response of adipocyte progenitors to glucocorticoids determines visceral obesity via the TEAD1-miR-27b-PRDM16 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifan Lv,Fan Xia,Jing Yu,Yunlu Sheng,Yi Jin,Yanqiang Li,Guoxian DingPubMed 37574723
- Nucleic acids research 50:11635-116532022Glucocorticoids unmask silent non-coding genetic risk variants for common diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThanh Thanh L Nguyen,Huanyao Gao,Duan Liu,Trudy Janice Philips,Zhenqing Ye,Jeong-Heon Lee,Geng-Xian Shi,Kaleigh Copenhaver,Lingxin Zhang,Lixuan Wei,Jia Yu,Huan Zhang,Abhijeet Barath,Maggie Luong,Cheng Zhang,Alexandre Gaspar-Maia,Hu Li,Liewei Wang,Tamas Ordog,Richard M WeinshilboumPubMed 36399508
- Translational psychiatry 12:4582022Two kinds of transcription factors mediate chronic morphine-induced decrease in miR-105 in medial prefrontal cortex of rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunfang Zhang,Xinli Guo,Zhangyin Cai,Yan Pan,Hao Yang,Yali Fu,Zixuan Cao,Yaxian Wen,Chao Lei,Chenshan Chu,Yu Yuan,Dongyang Cui,Pengyu Gao,Bin Lai,Ping ZhengPubMed 36316324
- Scientific reports 11:65062021Metabolomics analysis of follicular fluid coupled with oocyte aspiration reveals importance of glucocorticoids in primate periovulatory follicle competency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSweta Ravisankar,Carol B Hanna,Kelsey E Brooks,Melinda J Murphy,Nash Redmayne,Junghyun Ryu,Jason M Kinchen,Shawn L Chavez,Jon D HenneboldPubMed 33753762
- The Journal of experimental medicine 218:2021Hepatic Gadd45β promotes hyperglycemia and glucose intolerance through DNA demethylation of PGC-1α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Wu,Yang Jiao,Yao Li,Jingjing Jiang,Lin Zhao,Menghui Li,Bin Li,Zheng Yan,Xuejin Chen,Xiaoying Li,Yan LuPubMed 33688917
- Science advances 7:2021Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuayu Zhu,Jun Li,Yize Li,Zhao Zheng,Hao Guan,Hongtao Wang,Ke Tao,Jiaqi Liu,Yunchuan Wang,Wanfu Zhang,Chao Li,Jie Li,Lintao Jia,Wendong Bai,Dahai HuPubMed 33627425
- The Journal of clinical investigation 130:3791-38042020Obesity-induced excess of 17-hydroxyprogesterone promotes hyperglycemia through activation of glucocorticoid receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Lu,E Wang,Ying Chen,Bing Zhou,Jiejie Zhao,Liping Xiang,Yiling Qian,Jingjing Jiang,Lin Zhao,Xuelian Xiong,Zhiqiang Lu,Duojiao Wu,Bin Liu,Jing Yan,Rong Zhang,Huijie Zhang,Cheng Hu,Xiaoying LiPubMed 32510471
- Scientific reports 9:27722019Extensive epigenomic integration of the glucocorticoid response in primary human monocytes and in vitro derived macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Wang et. al.PubMed 30809020
- American journal of physiology. Endocrinology and metabolism 314:E39-E522017Histone deacetylase inhibition ameliorates hypertension and hyperglycemia in a model of Cushing's syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHae-Ahm Lee,Seol-Hee Kang,Mina Kim,Eunjo Lee,Hyun-Min Cho,Eun-Kyung Moon,Inkyeom KimPubMed 28928236
- PLoS medicine 13:e10022002016IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NR3C1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
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Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Isoform Alpha. Has transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253). Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466). Isoform GR-P. Increases activity of isoform Alpha. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity. Isoform 10. Has transcriptional activation activity. Isoform Alpha-C1. Has transcriptional activation activity. Isoform Alpha-C2. Has transcriptional activation activity. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903). Isoform Alpha-D1. Has transcriptional activation activity. Isoform Alpha-D2. Has transcriptional activation activity. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1
Recommended products
Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 30 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 200-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NR3C1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
- Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
- Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
- Associated diseases and disorders
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)
Immunofluorescence analysis of Glucocorticoid Receptor in MDA-MB-231 cells (serum-starved) and MDA-MB-231 cells serum-starved for 24 hours, followed by 1 μM Dexamethasone treatment for 2 hours using ab3579. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 2% BSA. The cells were labeled with ab3579 at 1/100 dilution in 0.1% BSA, incubated at 4°C overnight followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor®488 at 1/2000 dilution (Panel a: Green) in MDA-MB-231 treated cells. Nuclei (Panel b:Blue) were stained DAPI. F-actin (Panel c: Red) was stained with Rhodamine Phalloidin 1/300. (Panel d) represents the merged image showing nuclear localization of NR3C1 protein in MDA-MB-231 treated cells. (Panel e) represents the merged image of MDA-MB-231 untreated cells, that shows cytoplasmic localization of NR3C1 protein. (Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Western blot - Anti-Glucocorticoid Receptor antibody (ab3579)
All lanes: Western blot - Anti-Glucocorticoid Receptor antibody (ab3579) at 1 µg/mL
Lane 1: T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg/mL with 5% Milk in TBST
Lane 2: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 3: K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 5: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg with 5% Milk in TBST
SecondaryAll lanes: HRP conjugate at 1/1000 dilution
Predicted band size: 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)
Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)
Immunofluorescent analysis of ab3579 shows staining in HeLa (Human epithelial adenocarcinoma cell line) cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab3579at a dilution of 1/100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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