Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Snake samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 150-200.
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- Molecular psychiatry :2019Genome-wide methylation in alcohol use disorder subjects: implications for an epigenetic regulation of the cortico-limbic glucocorticoid receptors (NR3C1).Applications:Unspecified applicationReactive species:Unspecified reactive speciesEleonora Gatta et. al.PubMed 31239533
- Journal of cellular and molecular medicine 23:670-6792018Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Nettersheim,Daniel Berger,Sina Jostes,Margaretha Skowron,Hubert SchorlePubMed 30460772
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NR3C1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Expected | Expected |
| Snake | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2500 | Notes - |
Species Mouse | Dilution info 1/500 - 1/2500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Snake | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Snake | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Snake | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Isoform Alpha. Has transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253). Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466). Isoform GR-P. Increases activity of isoform Alpha. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity. Isoform 10. Has transcriptional activation activity. Isoform Alpha-C1. Has transcriptional activation activity. Isoform Alpha-C2. Has transcriptional activation activity. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903). Isoform Alpha-D1. Has transcriptional activation activity. Isoform Alpha-D2. Has transcriptional activation activity. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1
Recommended products
Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Snake samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NR3C1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
- Purity
- Whole antiserum
- Specificity
This antibody detects both the unactivated and activated forms of GR.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
- Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
- Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
- Associated diseases and disorders
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Glucocorticoid Receptor antibody (ab3671)
All lanes: Western blot - Anti-Glucocorticoid Receptor antibody (ab3671) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) membrane enriched extract at 30 µg
Lane 2: MCF7 (human breast adenocarcinoma cell line) membrane enriched extract at 30 µg
Lane 3: T-47D membrane enriched extract at 30 µg
Lane 4: MDA-MB-231 (human breast adenocarcinoma cell line) membrane enriched extract at 30 µg
Lane 5: HeLa (human epithelial cell line from cervix adenocarcinoma) membrane enriched extract at 30 µg
Lane 6: Mouse brain tissue extract at 30 µg
Predicted band size: 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3671)
Immunocytochemistry/Immunofluorescence analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3671)
Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3671)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial adenocarcinoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3671)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Thamnophis sirtalis (Common garter snake) brain tissue sections labeling Glucocorticoid Receptor with ab3671 at 1/250. Samples were blocked with 10% goat serum in 0.1M PBS. Samples were incubated with the primary antibody for 48 hours at 4°C. A biotin-conjugated goat anti-rabbit was used as the secondary antibody.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Glucocorticoid Receptor antibody (ab3671)
Glucocorticoid Receptor western blot using anti-Glucocorticoid Receptor antibody ab3671. Publication image and figure legend from Nettersheim, D., Berger, D., et al., 2019, J Cell Mol Med, PubMed 30460772.
ab3671 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3671 please see the product overview.
(A) Quantitative RT‐PCR (qRT‐PCR) analysis of indicated genes in TCam‐2, 2102EP and JAR cells treated with 50 μmol/L dexamethasone for 8 days. (B) Left side: qRT‐PCR analysis of indicated markers in TCam‐2, 2102EP and JAR cells after combinatory treatment with 10 nmol/L romidepsin and 100 μmol/L dexamethasone. Right side: XTT assay measuring viability of TCam‐2, 2102EP and JAR cells after individual or combinatory treatment with 2 nmol/L romidepsin and 50/100/200 μmol/L dexamethasone. (C) Western blot analysis of GR expression in GCT cell lines (left side) as well as in seminomas, ECs and normal testis tissue (NTT) (right side) 16 h after 10 nmol/L romidepsin treatment
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