Anti-Glucocorticoid Receptor antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3671)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial adenocarcinoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3671)

Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (AB3671)

Immunocytochemistry/Immunofluorescence analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (AB3671)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Thamnophis sirtalis (Common garter snake) brain tissue sections labeling Glucocorticoid Receptor with ab3671 at 1/250. Samples were blocked with 10% goat serum in 0.1M PBS. Samples were incubated with the primary antibody for 48 hours at 4°C. A biotin-conjugated goat anti-rabbit was used as the secondary antibody.

• WB

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Western blot - Anti-Glucocorticoid Receptor antibody (AB3671)

All lanes:

Western blot - Anti-Glucocorticoid Receptor antibody (ab3671) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) membrane enriched extract at 30 µg

Lane 2:

MCF7 (human breast adenocarcinoma cell line) membrane enriched extract at 30 µg

Lane 3:

T-47D membrane enriched extract at 30 µg

Lane 4:

MDA-MB-231 (human breast adenocarcinoma cell line) membrane enriched extract at 30 µg

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) membrane enriched extract at 30 µg

Lane 6:

Mouse brain tissue extract at 30 µg

Predicted band size: 85 kDa

false

• WB

CiteAb

Western blot - Anti-Glucocorticoid Receptor antibody (AB3671)

Glucocorticoid Receptor western blot using anti-Glucocorticoid Receptor antibody ab3671. Publication image and figure legend from Nettersheim, D., Berger, D., et al., 2019, J Cell Mol Med, PubMed 30460772.

ab3671 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3671 please see the product overview.

(A) Quantitative RT‐PCR (qRT‐PCR) analysis of indicated genes in TCam‐2, 2102EP and JAR cells treated with 50 μmol/L dexamethasone for 8 days. (B) Left side : qRT‐PCR analysis of indicated markers in TCam‐2, 2102EP and JAR cells after combinatory treatment with 10 nmol/L romidepsin and 100 μmol/L dexamethasone. Right side : XTT assay measuring viability of TCam‐2, 2102EP and JAR cells after individual or combinatory treatment with 2 nmol/L romidepsin and 50/100/200 μmol/L dexamethasone. (C) Western blot analysis of GR expression in GCT cell lines (left side) as well as in seminomas, ECs and normal testis tissue (NTT) (right side) 16 h after 10 nmol/L romidepsin treatment

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