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Mouse Monoclonal Glucocorticoid Receptor antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 52 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Nr3c1.

Isotype

IgG2

Host species

Mouse

Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

Full Length Protein corresponding to Rat Nr3c1. The exact immunogen used to generate this antibody is proprietary information. Database link: P06536

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Expected
Tested
Mouse
Tested
Tested
Expected
Rat
Expected
Expected
Expected
Amphibian
Not recommended
Not recommended
Not recommended
Bird
Not recommended
Not recommended
Not recommended
Primates
Not recommended
Not recommended
Not recommended

Images

Tested
Tested

Species
Mouse
Dilution info
0.5-1 µg for 106 Cells
Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
0.5-1 µg for 106 Cells
Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
0.5-1 µg for 106 Cells
Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Amphibian
Dilution info
-
Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species
Bird
Dilution info
-
Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species
Primates
Dilution info
-
Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Mouse
Dilution info
5 µg/mL
Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Expected
Expected

Species
Human
Dilution info
-
Notes

-

Species
Rat
Dilution info
5 µg/mL
Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Not recommended
Not recommended

Species
Amphibian
Dilution info
-
Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Species
Bird
Dilution info
-
Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Species
Primates
Dilution info
-
Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Tested
Tested

Species
Human
Dilution info
1/50 - 1/500
Notes

-

Expected
Expected

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
1/50 - 1/500
Notes

-

Not recommended
Not recommended

Species
Amphibian, Bird, Primates
Dilution info
-
Notes

-

Target data

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Mouse Monoclonal Glucocorticoid Receptor antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 52 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Nr3c1.

Key facts

Isotype

IgG2

Form

Liquid

Clonality

Monoclonal

Immunogen

Full Length Protein corresponding to Rat Nr3c1. The exact immunogen used to generate this antibody is proprietary information. Database link: P06536

Clone number

BuGR2

Purification technique

Affinity purification Protein A

Specificity

Immunocytochemical staining of GR in L929 cells with this antibody results in staining of both the cytoplasm and nucleus, even in the presence of hormone.
This antibody, using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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