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Mouse Monoclonal Glucocorticoid Receptor antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 52 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Nr3c1.


Images

Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (AB2768), expandable thumbnail
  • Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (AB2768), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (AB2768), expandable thumbnail
  • Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (AB2768), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (AB2768), expandable thumbnail

Publications

Key facts

Isotype

IgG2

Host species

Mouse

Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Rat Nr3c1. The exact immunogen used to generate this antibody is proprietary information. Database link P06536

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Expected
Tested
Mouse
Tested
Tested
Expected
Rat
Expected
Expected
Expected
Amphibian
Not recommended
Not recommended
Not recommended
Bird
Not recommended
Not recommended
Not recommended
Primates
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

0.5-1 µg for 106 Cells

Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

0.5-1 µg for 106 Cells

Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat

Dilution info

0.5-1 µg for 106 Cells

Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Amphibian

Dilution info

-

Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species

Bird

Dilution info

-

Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Species

Primates

Dilution info

-

Notes

ab18414 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Mouse

Dilution info

5 µg/mL

Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Expected
Expected

Species

Human

Dilution info

-

Notes

-

Species

Rat

Dilution info

5 µg/mL

Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Not recommended
Not recommended

Species

Amphibian

Dilution info

-

Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Species

Bird

Dilution info

-

Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Species

Primates

Dilution info

-

Notes

Using enzymatic digestion analysis detects a band of approximately 97 kDa, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment (predicted molecular weight: 86 kDa).

Tested
Tested

Species

Human

Dilution info

1/50 - 1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

1/50 - 1/500

Notes

-

Not recommended
Not recommended

Species

Amphibian, Bird, Primates

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).Isoform AlphaHas transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).Isoform BetaActs as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253).Isoform Alpha-2Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466).Isoform GR-PIncreases activity of isoform Alpha.Isoform Alpha-BMore effective than isoform Alpha in transcriptional activation, but not repression activity.Isoform 10Has transcriptional activation activity.Isoform Alpha-C1Has transcriptional activation activity.Isoform Alpha-C2Has transcriptional activation activity.Isoform Alpha-C3Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903).Isoform Alpha-D1Has transcriptional activation activity.Isoform Alpha-D2Has transcriptional activation activity.Isoform Alpha-D3Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).

Alternative names

Recommended products

Mouse Monoclonal Glucocorticoid Receptor antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 52 publications. Immunogen corresponding to Full Length Protein corresponding to Rat Nr3c1.

Key facts

Isotype

IgG2

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Rat Nr3c1. The exact immunogen used to generate this antibody is proprietary information. Database link P06536
Clone number

BuGR2

Purification technique

Affinity purification Protein A

Specificity

Immunocytochemical staining of GR in L929 cells with this antibody results in staining of both the cytoplasm and nucleus, even in the presence of hormone.
This antibody, using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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8 product images

  • Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Flow cytometry analysis of Glucocorticoid Receptor showing positive staining in the nucleus and cytoplasm of NIH/3T3 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2768 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Flow cytometry analysis of Glucocorticoid Receptor showing positive staining in the nucleus and cytoplasm of Jurkat cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and then incubated with ab2768 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Immunocytochemistry/Immunofluorescence analysis of Glucocorticoid Receptor shows staining in A549 cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2768 (1:100) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Flow cytometry analysis of Glucocorticoid Receptor showing positive staining in the nucleus and cytoplasm of Hela cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2768 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Immunocytochemistry/Immunofluorescence analysis of Glucocorticoid Receptor shows staining in HeLa cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2768 (1:100) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Immunocytochemistry/Immunofluorescence analysis of Glucocorticoid Receptor shows staining in U251 cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2768 (1:100) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Overlay histogram showing Jurkat cells stained with ab2768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2768, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2 (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768), expandable thumbnail

    Western blot - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Western blot of glucocorticoid receptor on mouse liver extract using ab2768. Western blot of glucocorticoid receptor on mouse liver extract using ab2768.

    All lanes: Western blot - Anti-Glucocorticoid Receptor antibody [BuGR2] (ab2768)

    Predicted band size: 85 kDa

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