Rabbit Recombinant Monoclonal Glucocorticoid Receptor antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Transfected cell lysate - Human, Mouse, Rat samples.
Images
![Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--western-blot-img99822.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117317.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130968.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102382.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127641.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (AB223138)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Expected | Expected | Expected | Tested |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell lysate - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
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Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).Isoform AlphaHas transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).Isoform BetaActs as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253).Isoform Alpha-2Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466).Isoform GR-PIncreases activity of isoform Alpha.Isoform Alpha-BMore effective than isoform Alpha in transcriptional activation, but not repression activity.Isoform 10Has transcriptional activation activity.Isoform Alpha-C1Has transcriptional activation activity.Isoform Alpha-C2Has transcriptional activation activity.Isoform Alpha-C3Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903).Isoform Alpha-D1Has transcriptional activation activity.Isoform Alpha-D2Has transcriptional activation activity.Isoform Alpha-D3Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1
Recommended products
Rabbit Recombinant Monoclonal Glucocorticoid Receptor antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Transfected cell lysate - Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19621
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab223138 is the carrier-free version of Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
- Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
- Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
- Associated diseases and disorders
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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8 product images
![Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--western-blot-img99822.jpg "Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
Lanes 1-4: Merged signal (red and green). Green - Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 observed at 90-100 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 Anti-Glucocorticoid Receptor antibody [EPR19621] was shown to specifically react with Glucocorticoid Receptor in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human NR3C1 (Glucocorticoid Receptor) knockout HeLa cell line ab261766 (knockout cell lysate Human NR3C1 (Glucocorticoid Receptor) knockout HeLa cell lysate ab257009) was used. Wild-type and Glucocorticoid Receptor knockout samples were subjected to SDS-PAGE. Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Glucocorticoid Receptor antibody [EPR19621] (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: NR3C1 knockout HeLa cell lysate at 20 µg
Lane 3: Wild-type A549 cell lysate at 20 µg
Lane 4: NR3C1 knockout A549 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 90-100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117317.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Glucocorticoid Receptor with Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the Human glioma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130968.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Glucocorticoid Receptor with Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the cervix carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102382.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glucocorticoid Receptor with Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on hepatocytes of the mouse liver is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127641.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Glucocorticoid Receptor with Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on rat hippocampus is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunocytochemistry-immunofluorescence-img36083.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Glucocorticoid Receptor with Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
The results show signal translocation after dexamethasone (100 nM for 2 hours) treatment on Hela cells. PMID: 24291004.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
![Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--flow-cytometry-intracellular-img26654.jpg "Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Glucocorticoid Receptor with Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 at 1/500 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
![Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--flow-cytometry-intracellular-img439602.jpg "Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127).
Flow cytometry overlay histogram showing wild-type (WT) HeLa (green line) and NR3C1 knockout (KO) HeLa stained with Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 mins. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody Anti-Glucocorticoid Receptor antibody [EPR19621] ab183127 (1x 106 in 100μl at 0.04 μg/ml (1/54,850 dilution) for 30 mins at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilution for 30 mins at 22°C
Isotype control antibody Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control was used at the same concentration and conditions as the primary antibody (HeLa WT - black line, NR3C1 KO HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in HeLa WT Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 mins under the same conditions.
![Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--immunocytochemistry-immunofluorescence-img36083.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
![Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--flow-cytometry-intracellular-img26654.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
![Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138), expandable thumbnail](https://content.abcam.com/products/images/glucocorticoid-receptor-antibody-epr19621-bsa-and-azide-free-ab223138--flow-cytometry-intracellular-img439602.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Glucocorticoid Receptor antibody [EPR19621] - BSA and Azide free (ab223138)")
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