AB233165

Anti-Glucocorticoid Receptor beta antibody

Name: Anti-Glucocorticoid Receptor beta antibody (ab233165) | Abcam
Brand: Abcam Limited
SKU: AB233165

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(4 Publications)

Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR3C1 aa 550 to C-terminus.

View Alternative Names

GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor beta antibody (AB233165)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor beta antibody (AB233165)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Glucocorticoid Receptor beta using ab233165 at 20μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor beta antibody (AB233165)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Glucocorticoid Receptor beta using ab233165 at 20μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Glucocorticoid Receptor beta antibody (AB233165)

All lanes:

Western blot - Anti-Glucocorticoid Receptor beta antibody (ab233165) at 2 µg/mL

All lanes:

Recombinant human Glucocorticoid Receptor beta

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 85 kDa

false

Supplier Data

Western blot - Anti-Glucocorticoid Receptor beta antibody (AB233165)

All lanes:

Western blot - Anti-Glucocorticoid Receptor beta antibody (ab233165) at 2 µg/mL

All lanes:

Pig brain tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NR3C1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04150

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Squirrel monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucocorticoid Receptor beta (GRβ) also known as NR3C1 isoform β is a variant of the glucocorticoid receptor. Unlike its counterpart GRα GRβ does not bind glucocorticoids due to differences in its ligand-binding domain. With an approximate mass of 90 kDa GRβ acts primarily as a dominant negative regulator of GRα. It is expressed in various tissues including skin lung and immune cells which suggests it has a wide-reaching influence on cellular responses to glucocorticoids.

Biological function summary

GRβ functions as a modulator of glucocorticoid signaling by inhibiting the activity of GRα. Though it does not bind glucocorticoids itself it can still form part of a receptor complex that affects transcriptional activities. By reducing GRα-mediated gene expression GRβ influences the body's response to stress and inflammation. Its role as a competitor to GRα shapes the regulation of genes involved in metabolism immune function and cellular growth.

Pathways

The regulatory role of GRβ in glucocorticoid signaling impacts the hypothalamic-pituitary-adrenal (HPA) axis which is important for stress response and homeostasis. GRβ interacts with proteins involved in the NF-kB pathway connecting it to the regulation of immune and inflammatory responses. Through its effect on these pathways GRβ exerts control over the transcriptional regulation shared with GRα and other transcription factors influencing cellular processes like apoptosis and autophagy.

Alterations in GRβ expression or function link to conditions such as corticosteroid resistance and autoimmune diseases including rheumatoid arthritis. GRβ's interaction with GRα is significant because imbalances in their ratios can alter the usual glucocorticoid effects contributing to disease pathology. GRβ also connects to proteins in inflammatory signaling cascades making it an important player in understanding the molecular mechanisms underlying resistance and sensitivity to glucocorticoid therapy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for glucocorticoids (GC) (PubMed : 27120390, PubMed : 37478846). Has a dual mode of action : as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed : 28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed : 9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed : 25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).. Isoform Alpha. Has transcriptional activation and repression activity (PubMed : 11435610, PubMed : 15769988, PubMed : 15866175, PubMed : 17635946, PubMed : 19141540, PubMed : 19248771, PubMed : 20484466, PubMed : 21664385, PubMed : 23820903). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127). Promotes accurate chromosome segregation during mitosis (PubMed : 25847991). May act as a tumor suppressor (PubMed : 25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).. Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed : 20484466, PubMed : 7769088, PubMed : 8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed : 19248771, PubMed : 26711253). Loses this transcription modulator function on its own (PubMed : 20484466). Has no hormone-binding activity (PubMed : 8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed : 26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed : 26711253).. Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed : 20484466).. Isoform GR-P. Increases activity of isoform Alpha.. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity.. Isoform 10. Has transcriptional activation activity.. Isoform Alpha-C1. Has transcriptional activation activity.. Isoform Alpha-C2. Has transcriptional activation activity.. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127, PubMed : 23820903).. Isoform Alpha-D1. Has transcriptional activation activity.. Isoform Alpha-D2. Has transcriptional activation activity.. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127).

See full target information NR3C1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36674967

2023

Diclofenac Disrupts the Circadian Clock and through Complex Cross-Talks Aggravates Immune-Mediated Liver Injury-A Repeated Dose Study in Minipigs for 28 Days.

Applications

Unspecified application

Species

Unspecified reactive species

Saravanakumar Selvaraj,Jung-Hwa Oh,Seokjoo Yoon,Jürgen Borlak

Frontiers in immunology 13:905727 PubMed35865549

2022

Increased Interleukin-17 and Glucocorticoid Receptor-β Expression in Interstitial Lung Diseases and Corticosteroid Insensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yu Lo,Chun-Hua Wang,Chih-Wei Wang,Chih-Jung Chen,Hung-Yu Huang,Fu-Tsai Chung,Yu-Chen Huang,Chang-Wei Lin,Chung-Shu Lee,Chun-Yu Lin,Chiung-Hung Lin,Po-Jui Chang,Ting-Yu Lin,Chih-Chen Heh,Jung-Ru He,Kian Fan Chung

Bioengineered 13:10765-10773 PubMed35475470

2022

Long non-coding RNA placenta‑specific protein 2 regulates the chemosensitivity of cancer cells to cisplatin in hepatocellular carcinoma (HCC) by sponging microRNA-96 to upregulate X-linked inhibitor of apoptosis protein.

Applications

Unspecified application

Species

Unspecified reactive species

Huixiong Wang,Xin Zhang,Xiaoting Chen,Shengbin Zhang,Zhelin Yun,Qiang Gao,Haitao Sheng,Junjie Wang

Immunity, inflammation and disease 8:605-614 PubMed32870597

2020

Increased lipopolysaccharide content is positively correlated with glucocorticoid receptor-beta expression in chronic rhinosinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Shui-Bin Wang,Shi-Ming Chen,Ke-Sheng Zhu,Bin Zhou,Long Chen,Xiao-Yan Zou

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Glucocorticoid Receptor beta antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor beta antibody (AB233165)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor beta antibody (AB233165)

Western blot - Anti-Glucocorticoid Receptor beta antibody (AB233165)

Western blot - Anti-Glucocorticoid Receptor beta antibody (AB233165)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise