Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR3C1 aa 550 to C-terminus.
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Publications
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- International journal of molecular sciences 24:2023Diclofenac Disrupts the Circadian Clock and through Complex Cross-Talks Aggravates Immune-Mediated Liver Injury-A Repeated Dose Study in Minipigs for 28 Days.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaravanakumar Selvaraj,Jung-Hwa Oh,Seokjoo Yoon,Jürgen BorlakPubMed 36674967
- Frontiers in immunology 13:9057272022Increased Interleukin-17 and Glucocorticoid Receptor-β Expression in Interstitial Lung Diseases and Corticosteroid Insensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yu Lo,Chun-Hua Wang,Chih-Wei Wang,Chih-Jung Chen,Hung-Yu Huang,Fu-Tsai Chung,Yu-Chen Huang,Chang-Wei Lin,Chung-Shu Lee,Chun-Yu Lin,Chiung-Hung Lin,Po-Jui Chang,Ting-Yu Lin,Chih-Chen Heh,Jung-Ru He,Kian Fan ChungPubMed 35865549
- Immunity, inflammation and disease 8:605-6142020Increased lipopolysaccharide content is positively correlated with glucocorticoid receptor-beta expression in chronic rhinosinusitis with nasal polyps.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShui-Bin Wang et. al.PubMed 32870597
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NR3C1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Tested | Expected |
| Rabbit | Predicted | Predicted |
| Squirrel monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Guinea pig, Orangutan, Squirrel monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Guinea pig, Orangutan, Squirrel monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Isoform Alpha. Has transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253). Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466). Isoform GR-P. Increases activity of isoform Alpha. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity. Isoform 10. Has transcriptional activation activity. Isoform Alpha-C1. Has transcriptional activation activity. Isoform Alpha-C2. Has transcriptional activation activity. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903). Isoform Alpha-D1. Has transcriptional activation activity. Isoform Alpha-D2. Has transcriptional activation activity. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1
Recommended products
Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR3C1 aa 550 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NR3C1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glucocorticoid Receptor beta (GRβ) also known as NR3C1 isoform β is a variant of the glucocorticoid receptor. Unlike its counterpart GRα GRβ does not bind glucocorticoids due to differences in its ligand-binding domain. With an approximate mass of 90 kDa GRβ acts primarily as a dominant negative regulator of GRα. It is expressed in various tissues including skin lung and immune cells which suggests it has a wide-reaching influence on cellular responses to glucocorticoids.
- Biological function summary
GRβ functions as a modulator of glucocorticoid signaling by inhibiting the activity of GRα. Though it does not bind glucocorticoids itself it can still form part of a receptor complex that affects transcriptional activities. By reducing GRα-mediated gene expression GRβ influences the body's response to stress and inflammation. Its role as a competitor to GRα shapes the regulation of genes involved in metabolism immune function and cellular growth.
- Pathways
The regulatory role of GRβ in glucocorticoid signaling impacts the hypothalamic-pituitary-adrenal (HPA) axis which is important for stress response and homeostasis. GRβ interacts with proteins involved in the NF-kB pathway connecting it to the regulation of immune and inflammatory responses. Through its effect on these pathways GRβ exerts control over the transcriptional regulation shared with GRα and other transcription factors influencing cellular processes like apoptosis and autophagy.
- Associated diseases and disorders
Alterations in GRβ expression or function link to conditions such as corticosteroid resistance and autoimmune diseases including rheumatoid arthritis. GRβ's interaction with GRα is significant because imbalances in their ratios can alter the usual glucocorticoid effects contributing to disease pathology. GRβ also connects to proteins in inflammatory signaling cascades making it an important player in understanding the molecular mechanisms underlying resistance and sensitivity to glucocorticoid therapy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor beta antibody (ab233165)
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Glucocorticoid Receptor beta using ab233165 at 20μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor beta antibody (ab233165)
Formalin-fixed, paraffin-embedded human stomach tissue stained for Glucocorticoid Receptor beta using ab233165 at 20μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Glucocorticoid Receptor beta antibody (ab233165)
All lanes: Western blot - Anti-Glucocorticoid Receptor beta antibody (ab233165) at 2 µg/mL
All lanes: Recombinant human Glucocorticoid Receptor beta
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 85 kDa
Western blot - Anti-Glucocorticoid Receptor beta antibody (ab233165)
All lanes: Western blot - Anti-Glucocorticoid Receptor beta antibody (ab233165) at 2 µg/mL
All lanes: Pig brain tissue lysate
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 85 kDa
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