Rabbit Polyclonal Glucocorticoid Receptor phospho S211 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 phospho S211.
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- Cell death & disease 14:1462023Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jeong Kim,Gee Euhn Choi,Chang Woo Chae,Jae Ryong Lim,Young Hyun Jung,Jee Hyeon Yoon,Ji Yong Park,Ho Jae HanPubMed 36810730
- Biomolecules 11:2021β-Ionone Attenuates Dexamethasone-Induced Suppression of Collagen and Hyaluronic Acid Synthesis in Human Dermal Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDabin Choi,Wesuk Kang,Soyoon Park,Bomin Son,Taesun ParkPubMed 33919331
- International journal of molecular sciences 19:2018Glucocorticoid Receptor Binding Inhibits an Intronic Enhancer and is Disrupted by rs4742170 (T) Allele Associated with Specific Wheezing Phenotype in Early Childhood.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlisa M Gorbacheva et. al.PubMed 30544846
- Molecular pharmacology 94:1031-10462018Long-Acting -Adrenoceptor Agonists Enhance Glucocorticoid Receptor (GR)-Mediated Transcription by Gene-Specific Mechanisms Rather Than Generic Effects via GR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher F Rider et. al.PubMed 29959223
- Scientific reports 7:41352017Prednisolone induces apoptosis in corneal epithelial cells through the intrinsic pathway.
- Journal of immunology (Baltimore, Md. : 1950) 197:3782-37912016Cytokine-Induced Glucocorticoid Resistance from Eosinophil Activation: Protein Phosphatase 5 Modulation of Glucocorticoid Receptor Phosphorylation and Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKonrad Pazdrak et. al.PubMed 27742828
- BMC veterinary research 11:1232015Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of 3β-hydroxysteroid dehydrogenase in the liver of preweaning piglets.
- The Biochemical journal 466:163-762014Decreased adipogenesis and adipose tissue in mice with inactivated protein phosphatase 5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWright Jacob,Doron Rosenzweig,Cristina Vázquez-Martin,Suzanne L Duce,Patricia T W CohenPubMed 25437352
- American journal of respiratory cell and molecular 40:223-302008IFN-gamma reverses IL-2- and IL-4-mediated T-cell steroid resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Goleva et. al.PubMed 18776133
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human NR3C1 phospho S211. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).Isoform AlphaHas transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).Isoform BetaActs as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253).Isoform Alpha-2Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466).Isoform GR-PIncreases activity of isoform Alpha.Isoform Alpha-BMore effective than isoform Alpha in transcriptional activation, but not repression activity.Isoform 10Has transcriptional activation activity.Isoform Alpha-C1Has transcriptional activation activity.Isoform Alpha-C2Has transcriptional activation activity.Isoform Alpha-C3Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903).Isoform Alpha-D1Has transcriptional activation activity.Isoform Alpha-D2Has transcriptional activation activity.Isoform Alpha-D3Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).
Alternative names
GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1
Recommended products
Rabbit Polyclonal Glucocorticoid Receptor phospho S211 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 phospho S211.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human NR3C1 phospho S211. The exact immunogen used to generate this antibody is proprietary information. Database link P04150
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
- Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
- Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
- Associated diseases and disorders
Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
All lanes: Western blot - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189) at 1/500 dilution
Lane 1: extracts from HeLa cells treated with PMA (125ng/ml, 30min), with the immunising phosphpeptide.
Lane 2: extracts from HeLa cells treated with PMA (125ng/ml, 30min).
Predicted band size: 85 kDa
Observed band size: 86 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
ab55189 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing phospho-peptide; paraffin embedded.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
Glucocorticoid Receptor (phospho S211) western blot using anti-Glucocorticoid Receptor (phospho S211) antibody ab55189. Publication image and figure legend from Ryu, J. S., Ko, J. H., et al., 2017, Sci Rep, PubMed 28646191.
ab55189 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab55189 please see the product overview.
Glucocorticoid receptor (GR) mediates the effects of prednisolone. hCECs were cultured with or without 10 μg/ml prednisolone for 3 days and subjected to assays. (A) Real-time RT PCR assay for GR expression. (B) Representative image and densitometry analysis of western blot for GR relative to β-actin. (C,D) MTT assay and BrdU uptake assay to measure cell metabolic activity and proliferation, respectively. (E–G) Flow cytometric analysis for ANX+PI+ cells to measure cell apoptosis. The GR antagonist RU-38486 was added to some cultures (E). The percentage of ANX+PI+ cells was measured in hCECs with GR siRNA or scrambled (SCR) siRNA transfection (F,G). Data are presented as mean ±/+ SD from five independent sets of experiments including at least five samples per a set. **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant.
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