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AB88056

Anti-Glucokinase antibody

5

(1 Review)

|

(9 Publications)

Rabbit Polyclonal HXK4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications.

View Alternative Names

Hexokinase-4, HK4, Glucokinase, Hexokinase type IV, Hexokinase-D, HK IV, GCK

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Glucokinase antibody (AB88056)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Glucokinase antibody (AB88056)

ab88056 staining Glucokinase in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab88056 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Western blot - Anti-Glucokinase antibody (AB88056)
  • WB

Unknown

Western blot - Anti-Glucokinase antibody (AB88056)

All lanes:

Western blot - Anti-Glucokinase antibody (ab88056) at 1 µg/mL

All lanes:

Liver (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 8min

Western blot - Anti-Glucokinase antibody (AB88056)
  • WB

Unknown

Western blot - Anti-Glucokinase antibody (AB88056)

All lanes:

Western blot - Anti-Glucokinase antibody (ab88056) at 1 µg/mL

All lanes:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg/mL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of our polyclonal antibody, ab88056 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab155962.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucokinase also known as GCK is an enzyme with a mass around 50 kDa. It functions by catalyzing the phosphorylation of glucose to glucose-6-phosphate an important step in glycolysis. Glucokinase displays a higher affinity for glucose as compared to other hexokinases which is indicated by its specific kinetic property known as the Km value. This enzyme is mainly expressed in the liver and pancreatic beta-cells where it plays an important role in glucose metabolism. Additionally glucokinase can also be found in the hypothalamus indicating its broader reach in carbohydrate sensing.
Biological function summary

Glucokinase acts as a glucose sensor regulating blood sugar levels and insulin secretion. This process does not involve the enzyme as part of a larger complex. Instead glucokinase functions independently to sense glucose concentrations and adjusts the rate of glucose phosphorylation accordingly. Its activity significantly affects the balance of glucose uptake and energy production especially during postprandial states when glucose levels rise. Glucokinase activity is therefore important to maintaining glucose homeostasis.

Pathways

Glucokinase plays a central role in the glycolytic pathway and interacts with the insulin signaling pathway. Within these pathways it influences how the liver stores glucose as glycogen and controls insulin release from pancreatic beta-cells. Glucokinase activity is modulated by glucose levels and is closely related to insulin receptor substrates that affect downstream insulin signaling. Its function is important for the proper regulation of these pathways linking glucose sensing with metabolic control in varied tissues.

Glucokinase is pivotal in the context of diabetes mellitus and hyperglycemia. Mutations or dysregulation of glucokinase can lead to different forms of diabetes including maturity-onset diabetes of the young type 2 (MODY2). Glucokinase inhibitors and activators are being explored as potential therapeutic agents for modulating glucose levels in diabetics. Additionally its role interfaces with the functions of insulin tying glucokinase closely to conditions of insulin resistance and pancreatic dysfunction. Understanding these connections is instrumental for developing targeted treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively) (PubMed : 11916951, PubMed : 15277402, PubMed : 17082186, PubMed : 18322640, PubMed : 19146401, PubMed : 25015100, PubMed : 7742312, PubMed : 8325892). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (By similarity). Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues (PubMed : 11916951, PubMed : 15277402, PubMed : 18322640, PubMed : 25015100, PubMed : 8325892). Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (By similarity). In pancreas, plays an important role in modulating insulin secretion (By similarity). In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (By similarity). Required to provide D-glucose 6-phosphate for the synthesis of glycogen (PubMed : 8878425). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed : 7742312).
See full target information Hexokinase-4
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Stem cells translational medicine 13:776-790 PubMed38864709

2024

Mesenchymal stromal cells ameliorate mitochondrial dysfunction in α cells and hyperglucagonemia in type 2 diabetes via SIRT1/FoxO3a signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Song,Lingshu Wang,Liming Wang,Xinghong Guo,Qin He,Chen Cui,Huiqing Hu,Nan Zang,Mengmeng Yang,Fei Yan,Kai Liang,Chuan Wang,Fuqiang Liu,Yujing Sun,Zheng Sun,Hong Lai,Xinguo Hou,Li Chen

Stem cell research & therapy 14:314 PubMed37907977

2023

Extracellular matrix modulates the spatial hepatic features in hepatocyte-like cells derived from human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Faiza Farhan,Manjari Trivedi,Priscilla Di Wu,Wei Cui

Molecules (Basel, Switzerland) 27: PubMed35956917

2022

Acetyl-CoA Deficiency Is Involved in the Regulation of Iron Overload on Lipid Metabolism in Apolipoprotein E Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Luo,Lu Xiang,Lin Xiao

Antioxidants (Basel, Switzerland) 10: PubMed34204849

2021

Neonatal Vitamin C and Cysteine Deficiencies Program Adult Hepatic Glutathione and Specific Activities of Glucokinase, Phosphofructokinase, and Acetyl-CoA Carboxylase in Guinea Pigs' Livers.

Applications

Unspecified application

Species

Unspecified reactive species

Vitor Teixeira,Ibrahim Mohamed,Jean-Claude Lavoie

The EMBO journal 40:e106583 PubMed33459428

2021

Zonal human hepatocytes are differentially permissive to Plasmodium falciparum malaria parasites.

Applications

Unspecified application

Species

Unspecified reactive species

Annie S P Yang,Youri M van Waardenburg,Marga van de Vegte-Bolmer,Geert-Jan A van Gemert,Wouter Graumans,Johannes H W de Wilt,Robert W Sauerwein

Molecular therapy. Methods & clinical development 18:811-823 PubMed32953932

2020

Soluble Klotho Improves Hepatic Glucose and Lipid Homeostasis in Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Huiying Gu,Wei Jiang,Nan You,Xiaobing Huang,Yuming Li,Xuehui Peng,Rui Dong,Zheng Wang,Yinan Zhu,Ke Wu,Jing Li,Lu Zheng

Molecular metabolism 18:15-24 PubMed30348590

2018

Control of hepatic gluconeogenesis by Argonaute2.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yan,Zhen Wang,Christopher A Bishop,Karolin Weitkunat,Xiao Feng,Marcel Tarbier,Jiankai Luo,Marc R Friedländer,Ralph Burkhardt,Susanne Klaus,Thomas E Willnow,Matthew N Poy

Immunity 47:875-889.e10 PubMed29166588

2017

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Madhav Kishore,Kenneth C P Cheung,Hongmei Fu,Fabrizia Bonacina,Guosu Wang,David Coe,Eleanor J Ward,Alessandra Colamatteo,Maryam Jangani,Andrea Baragetti,Giuseppe Matarese,David M Smith,Robert Haas,Claudio Mauro,David C Wraith,Klaus Okkenhaug,Alberico L Catapano,Veronica De Rosa,Giuseppe D Norata,Federica M Marelli-Berg

Endocrinology 157:3788-3799 PubMed27501184

2016

Chronically Increased Amino Acids Improve Insulin Secretion, Pancreatic Vascularity, and Islet Size in Growth-Restricted Fetal Sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Laura D Brown,Melissa Davis,Sandra Wai,Stephanie R Wesolowski,William W Hay,Sean W Limesand,Paul J Rozance
View all publications
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Alternative Products

Primary Antibodies

AB155962

Anti-Glucokinase antibody [EPR10374]

RabMAb|Recombinant

Western blot - Anti-Glucokinase antibody [EPR10374] (AB155962)

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Anti-Glucokinase antibody [EPR10374] - BSA and Azide free

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Western blot - Anti-Glucokinase antibody [EPR10374] - BSA and Azide free (AB249216)

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Secondary Antibodies

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