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AB249216

Anti-Glucokinase antibody [EPR10374] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HXK4 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Hexokinase-4, HK4, Glucokinase, Hexokinase type IV, Hexokinase-D, HK IV, GCK

1 Images
Western blot - Anti-Glucokinase antibody [EPR10374] - BSA and Azide free (AB249216)
  • WB

Unknown

Western blot - Anti-Glucokinase antibody [EPR10374] - BSA and Azide free (AB249216)

This data was developed using ab155962, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Glucokinase antibody [EPR10374] (<a href='/en-us/products/primary-antibodies/glucokinase-antibody-epr10374-ab155962'>ab155962</a>) at 1/50000 dilution

All lanes:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10374

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249216 is the carrier-free version of ab155962.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucokinase also known as GCK is an enzyme with a mass around 50 kDa. It functions by catalyzing the phosphorylation of glucose to glucose-6-phosphate an important step in glycolysis. Glucokinase displays a higher affinity for glucose as compared to other hexokinases which is indicated by its specific kinetic property known as the Km value. This enzyme is mainly expressed in the liver and pancreatic beta-cells where it plays an important role in glucose metabolism. Additionally glucokinase can also be found in the hypothalamus indicating its broader reach in carbohydrate sensing.
Biological function summary

Glucokinase acts as a glucose sensor regulating blood sugar levels and insulin secretion. This process does not involve the enzyme as part of a larger complex. Instead glucokinase functions independently to sense glucose concentrations and adjusts the rate of glucose phosphorylation accordingly. Its activity significantly affects the balance of glucose uptake and energy production especially during postprandial states when glucose levels rise. Glucokinase activity is therefore important to maintaining glucose homeostasis.

Pathways

Glucokinase plays a central role in the glycolytic pathway and interacts with the insulin signaling pathway. Within these pathways it influences how the liver stores glucose as glycogen and controls insulin release from pancreatic beta-cells. Glucokinase activity is modulated by glucose levels and is closely related to insulin receptor substrates that affect downstream insulin signaling. Its function is important for the proper regulation of these pathways linking glucose sensing with metabolic control in varied tissues.

Glucokinase is pivotal in the context of diabetes mellitus and hyperglycemia. Mutations or dysregulation of glucokinase can lead to different forms of diabetes including maturity-onset diabetes of the young type 2 (MODY2). Glucokinase inhibitors and activators are being explored as potential therapeutic agents for modulating glucose levels in diabetics. Additionally its role interfaces with the functions of insulin tying glucokinase closely to conditions of insulin resistance and pancreatic dysfunction. Understanding these connections is instrumental for developing targeted treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively) (PubMed : 11916951, PubMed : 15277402, PubMed : 17082186, PubMed : 18322640, PubMed : 19146401, PubMed : 25015100, PubMed : 7742312, PubMed : 8325892). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant (By similarity). Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues (PubMed : 11916951, PubMed : 15277402, PubMed : 18322640, PubMed : 25015100, PubMed : 8325892). Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell (By similarity). In pancreas, plays an important role in modulating insulin secretion (By similarity). In liver, helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage (By similarity). Required to provide D-glucose 6-phosphate for the synthesis of glycogen (PubMed : 8878425). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed : 7742312).
See full target information Hexokinase-4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen
View all publications
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Product promise

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