Anti-Glucokinase antibody [EPR25847-150] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucokinase antibody [EPR25847-150] - BSA and Azide free (AB303613)

This data was developed using ab303612, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Glucokinase with ab303612 at 1/250 dilution (4.68 µg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab303612 at 4°C overnight. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucokinase antibody [EPR25847-150] - BSA and Azide free (AB303613)

This data was developed using ab303612, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Glucokinase with ab303612 at 1/250 dilution (4.68 µg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human pancreatic islet is observed. The section was incubated with ab303612 at 4°C overnight. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucokinase antibody [EPR25847-150] - BSA and Azide free (AB303613)

This data was developed using ab303612, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Glucokinase with ab303612 at 1/250 dilution (4.68 µg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human kidney is observed.

The section was incubated with ab303612 at 4°C overnight. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucokinase antibody [EPR25847-150] - BSA and Azide free (AB303613)

This data was developed using ab303612, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glucokinase with ab303612 at 1/250 dilution (4.68 µg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver is observed. The section was incubated with ab303612 at 4°C overnight. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucokinase antibody [EPR25847-150] - BSA and Azide free (AB303613)

This data was developed using ab303612, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Glucokinase with ab303612 at 1/250 dilution (4.68 µg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver is observed. The section was incubated with ab303612 for 10 min at room temerature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucokinase antibody [EPR25847-150] - BSA and Azide free (AB303613)

This data was developed using ab303612, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Glucokinase with ab303612 at 1/250 dilution (4.68 µg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse kidney is observed.

The section was incubated with ab303612 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.