AB106810

Anti-Glucose 6 Phosphate Dehydrogenase antibody

Name: Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810) | Abcam
Brand: Abcam Limited
SKU: AB106810

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(4 Publications)

Goat Polyclonal Glucose 6 Phosphate Dehydrogenase antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Glucose-6-phosphate 1-dehydrogenase aa 300-350.

View Alternative Names

Glucose-6-phosphate 1-dehydrogenase, G6PD

1 Images

Unknown

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB106810)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810) at 0.03 µg/mL

All lanes:

Human testis tissue lysate in RIPA buffer at 35 µg

Predicted band size: 59 kDa

Observed band size: 55-60 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Glucose-6-phosphate 1-dehydrogenase aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P11413

Specificity

ab106810 is expected to recognise isoforms NP_000393.4 and NP_001035810.1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab106810 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.

Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

See full target information Glucose-6-phosphate 1-dehydrogenase

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:82 PubMed36737598

2023

Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingli Liu,Jingyuan Xu,Linyao Lu,Lili Gao,Shengjuan Zhu,Yi Sui,Ting Cao,Tao Yang

Biological chemistry 402:1073-1085 PubMed34333885

2021

Characterization of the scavenger cell proteome in mouse and rat liver.

Applications

Unspecified application

Species

Unspecified reactive species

Martha Paluschinski,Cheng Jun Jin,Natalia Qvartskhava,Boris Görg,Marianne Wammers,Judith Lang,Karl Lang,Gereon Poschmann,Kai Stühler,Dieter Häussinger

Laryngoscope investigative otolaryngology 6:283-290 PubMed33869760

2021

Role of colony-forming tissue stem cells in the macula flava of the human vocal fold in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Kiminori Sato,Shun-Ichi Chitose,Kiminobu Sato,Fumihiko Sato,Takeharu Ono,Hirohito Umeno

Laryngoscope investigative otolaryngology 6:122-128 PubMed33614940

2021

Glycolytic activity of the tissue stem cells in the macula flava of the human vocal fold.

Applications

Unspecified application

Species

Unspecified reactive species

Kiminori Sato,Shun-Ichi Chitose,Kiminobu Sato,Fumihiko Sato,Takeharu Ono,Hirohito Umeno

View all publications

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Product promise

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Anti-Glucose 6 Phosphate Dehydrogenase antibody

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB106810)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise