AB993

Anti-Glucose 6 Phosphate Dehydrogenase antibody

Name: Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) | Abcam
Brand: Abcam Limited
SKU: AB993
Price: 505 USD
Availability: InStock
Rating: 4 (13 reviews)

(13 Reviews)

(65 Publications)

Rabbit Polyclonal Glucose 6 Phosphate Dehydrogenase antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human Glucose-6-phosphate 1-dehydrogenase aa 50-100.

View Alternative Names

Glucose-6-phosphate 1-dehydrogenase, G6PD

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB993)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB993)

ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Supplier Data

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB993)

Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 μg/ml.

Lane 1 : ab993 (batch 3) IP in NIH/3T3 whole cell lysate.

Lane 2 : ab993 (batch 4) IP in NIH/3T3 whole cell lysate.

Lane 3 : Control IgG IP in NIH/3T3 whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Predicted band size: 59 kDa

false

Supplier Data

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB993)

All lanes:

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 59 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Synthetic Peptide within Human Glucose-6-phosphate 1-dehydrogenase aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P11413

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Baboon": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.

Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

See full target information Glucose-6-phosphate 1-dehydrogenase

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Publications (65)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108247 PubMed39894218

2025

A BRAF-activated noncoding RNA attenuates clear cell renal cell carcinoma via repression of glucose-6-phosphate dehydrogenase.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Liu,Yueli Ni,Honggang Bai,Xiangjie Liu,Asif Shahzad,Kun Cui,Qiuxin Duan,Ziyuan Bai,Yurong Dong,Zihan Yi,Buqing Sai,Yingmin Kuang,Chen Guo,Yuechun Zhu,Qiao Zhang,Zhe Yang

Science advances 10:eado1662 PubMed39270011

2024

Clusters of lineage-specific genes are anchored by ZNF274 in repressive perinucleolar compartments.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Begnis,Julien Duc,Sandra Offner,Delphine Grun,Shaoline Sheppard,Olga Rosspopoff,Didier Trono

Nature communications 15:5857 PubMed38997257

2024

Glucose-6-phosphate dehydrogenase maintains redox homeostasis and biosynthesis in LKB1-deficient KRAS-driven lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Taijin Lan,Sara Arastu,Jarrick Lam,Hyungsin Kim,Wenping Wang,Samuel Wang,Vrushank Bhatt,Eduardo Cararo Lopes,Zhixian Hu,Michael Sun,Xuefei Luo,Jonathan M Ghergurovich,Xiaoyang Su,Joshua D Rabinowitz,Eileen White,Jessie Yanxiang Guo

Scientific reports 14:12143 PubMed38802677

2024

LncRNA TUG1 mediates microglial inflammatory activation by regulating glucose metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiang He,Ze Li,Wenjing Yu,Rongsiqing Luo,Jinyong Zhou,Jiawei He,Qi Chen,Zhenyan Song,Shaowu Cheng

Molecular medicine (Cambridge, Mass.) 30:69 PubMed38783226

2024

ECHDC2 inhibits the proliferation of gastric cancer cells by binding with NEDD4 to degrade MCCC2 and reduce aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancheng He,Jianfeng Yi,Li Ji,Lingchen Dai,Yu Chen,Wanjiang Xue

EBioMedicine 102:105078 PubMed38507875

2024

An integrative pan-cancer analysis of the molecular characteristics of dietary restriction in tumour microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyi Song,Jiaxing Wei,Yang Li,Wen Zhu,Zhiyuan Cai,Kunwei Li,Jingyue Wei,Jieyu Lu,Wanping Pan,Man Li

Analytical biochemistry 687:115445 PubMed38135241

2023

REAP+: A single preparation for rapid isolation of nuclei, cytoplasm, and mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Dos Santos,Monique Coelho Bion,Marie Goujon-Svrzic,Pamela Maher,Alcir Luiz Dafre

Nature communications 14:6208 PubMed37798264

2023

Acetylation-dependent coupling between G6PD activity and apoptotic signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wu,Natali H Muskat,Inbar Dvilansky,Omri Koren,Anat Shahar,Roi Gazit,Natalie Elia,Eyal Arbely

Cell reports. Medicine 4:101162 PubMed37597521

2023

Metabolic classification suggests the GLUT1/ALDOB/G6PD axis as a therapeutic target in chemotherapy-resistant pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Li,Shijie Tang,Xiaohan Shi,Jingwen Lv,Xueyuan Wu,Yehan Zhang,Huan Wang,Juan He,Yiqin Zhu,Yi Ju,Yajuan Zhang,Shiwei Guo,Weiwei Yang,Huiyong Yin,Luonan Chen,Dong Gao,Gang Jin

Medical oncology (Northwood, London, England) 40:145 PubMed37043051

2023

JMJD5 inhibits lung cancer progression by regulating glucose metabolism through the p53/TIGAR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guiling Liu,Hongyan Qi,Jing Shen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose 6 Phosphate Dehydrogenase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB993)

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB993)

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (AB993)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (65)

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