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AB210702

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668]

3

(1 Review)

|

(26 Publications)

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (ab210702) is a rabbit monoclonal antibody detecting Glucose 6 Phosphate Dehydrogenase in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Glucose-6-phosphate 1-dehydrogenase, G6PD

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue (left panel) and human gastric paracarcinoma (right panel) labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocaricoma, compared with weak cytoplasmic staining on the paired paracarcinoma stomach (PMID : 22012600). Both tissue sections are derived from the same patient sample. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Immunofluorescent analysis of methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on stroma of human liver (PMID : 24994855, PMID : 26583321). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Staining on hepatocellular carcinoma (PMID : 24994855, PMID : 26583321). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocarcinoma (PMID : 22012600). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Glucose 6 Phosphate Dehydrogenasewith ab210702 at 1/400 (red) compared with an Isotype control rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Immunofluorescent analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLA cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • IP

Supplier Data

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab210702 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab210702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab210702 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab210702 in HeLa whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (ab210702)

Predicted band size: 59 kDa

false

Exposure time: 30s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Glucose 6 Phosphate Dehydrogenase with ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat liver (PMID : 24994855, PMID : 26583321). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • WB

Supplier Data

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1/2/3 : 3 minutes; Lanes 4/5 : 3 seconds.

All lanes:

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (ab210702) at 1/5000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg

Lane 3:

Mouse testis lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 5:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)
  • WB

Supplier Data

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (AB210702)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1/2/3 : 3 minutes; Lane 4 : 15 seconds; Lanes 5/6 : 30 seconds; Lane 7 : 15 seconds.

All lanes:

Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (ab210702) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat liver lysate at 10 µg

Lane 3:

Rat spleen lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 6:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

  • Carrier free

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20668

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product details

What is this antibody validated in?
Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (ab210702) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Glucose 6 Phosphate Dehydrogenase?
Anti-Glucose 6 Phosphate Dehydrogenase [EPR20668] (ab210702) specifically detects a band for Glucose 6 Phosphate Dehydrogenase (UniProt: P11413) at a molecular weight of 59kDa.

Trusted by the scientific community
Anti-Glucose 6 Phosphate Dehydrogenase [EPR20668] (ab210702) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20668] also available for your convenience: ab210702, Carrier free - ab231828, ab322989, Carrier free - ab322995

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-Δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
See full target information Glucose-6-phosphate 1-dehydrogenase

Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Life metabolism 4:loaf028 PubMed41070195

2025

The brown fat-enriched exosomal miR-206-3p attenuates hepatic lipogenesis by decreasing pentose phosphate pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Jie Yang,Qiu-Kai Tang,Lei Wang,Yan-Jue Song,Zhen-Yu Xu,Xi-Ni Ma,Yang Liu,Shu-Wen Qian,Qi-Qun Tang,Yan Tang

World journal of surgical oncology 23:175 PubMed40307857

2025

Multiple-omics analysis of aggrephagy-related cellular patterns and development of an aggrephagy-related signature for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiafen Xie,Xiaoming Wang

Cancer medicine 13:e70284 PubMed39540710

2024

Predictive Prognostic Model for Hepatocellular Carcinoma Based on Seven Genes Participating in Arachidonic Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Gu,Jing Wang,Jun Guan,Guojun Li,Xiao Ma,Yanli Ren,Shanshan Wu,Chao Chen,Haihong Zhu

Cell death discovery 10:361 PubMed39138186

2024

METTL14-mediated m6A mRNA modification of G6PD promotes lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong Wu,Mengling Li,Yingxiao Wu,Qiongying Wei,Nanding Yu

The Journal of pharmacology and experimental therapeutics 391:119-129 PubMed39095205

2024

Primaquine-5,6-Orthoquinone Is Directly Hemolytic to Older G6PD Deficient RBCs in a Humanized Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina H Dziewulska-Cronk,Julie A Reisz,Ariel M Hay,Travis Nemkov,Francesca I Cendali,Aaron Issaian,Derek R Lamb,Mitasha S Palha,Eric A Legenzov,Joseph P Y Kao,Larry A Walker,Babu L Tekwani,Paul W Buehler,Angelo D'Alessandro,James C Zimring

Anti-cancer drugs 35:902-911 PubMed39012720

2024

Interference with PLA2G16 promotes cell cycle arrest and apoptosis and inhibits the reprogramming of glucose metabolism in multiple myeloma cells by modulating the Hippo/YAP signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Li,Yi Zhang,Xiaoyu Mou,Bo Huang,Xiaoqiang Fan

Clinical and experimental pharmacology & physiology 51:e13860 PubMed38584327

2024

NPAS2, transcriptionally activated by ARRB1, promotes the malignant behaviours of lung adenocarcinoma cells and regulates the reprogramming of glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglan Wang,Chunhong Huang,Yanbin Zheng,Xinjie Wu,Yutong Zhong

Heliyon 10:e25144 PubMed38322888

2024

HuaChanSu suppresses the growth of hepatocellular carcinoma cells by interfering with pentose phosphate pathway through down-regulation of G6PD enzyme activity and expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wu,Xue-Li Ge,Zi-Kai Geng,Hao Wu,Jing-Yi Yang,Shi-Rong Cao,Ai-Lin Yang

BMC cancer 24:157 PubMed38297250

2024

G6PD and machine learning algorithms as prognostic and diagnostic indicators of liver hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Li,Boshen Wang,Hao Li,Lu Kong,Baoli Zhu

iScience 26:108137 PubMed37867937

2023

A fingerprint of 2-[F]FDG radiometabolites - How tissue-specific metabolism beyond 2-[F]FDG-6-P could affect tracer accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Eva-Maria Patronas,Theresa Balber,Anne Miller,Barbara Katharina Geist,Antje Michligk,Chrysoula Vraka,Maximilian Krisch,Nataliya Rohr-Udilova,Arvand Haschemi,Helmut Viernstein,Marcus Hacker,Markus Mitterhauser
View all publications

Product promise

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