Name: Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free
SKU: ab231828
Rating: 4 (5 reviews)

Anti-Glucose 6 Phosphate Dehydrogenase [EPR20668] antibody (ab231828) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect Glucose 6 Phosphate Dehydrogenase in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Expected	Tested	Tested	Tested
Mouse	Predicted	Expected	Predicted	Predicted	Predicted
Rat	Predicted	Expected	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Associated Products

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8 products for Alternative Product

Target data

See full target information Glucose-6-phosphate 1-dehydrogenase

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

Alternative names

Glucose-6-phosphate 1-dehydrogenase, G6PD

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR20668
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Staining on hepatocellular carcinoma (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on stroma of human liver (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunofluorescent analysis of methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunofluorescent analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLA cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue (left panel) and human gastric paracarcinoma (right panel) labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocaricoma, compared with weak cytoplasmic staining on the paired paracarcinoma stomach (PMID: 22012600). Both tissue sections are derived from the same patient sample. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocarcinoma (PMID: 22012600). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 in HeLa whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
All lanes: Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702)
Predicted band size: 59 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat liver (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/400 (red) compared with an Isotype control rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).