Rabbit Recombinant Monoclonal Glucose 6 Phosphate Dehydrogenase antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120367.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103523.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)")
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunocytochemistry-immunofluorescence-img73529.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)")
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunocytochemistry-immunofluorescence-img73034.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32075.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (AB231828)")
Publications
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- The Journal of clinical investigation 134:2024Inhibiting the NADase CD38 improves cytomegalovirus-specific CD8+ T cell functionality and metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNils Mülling,Felix M Behr,Graham A Heieis,Kristina Boss,Suzanne van Duikeren,Floortje J van Haften,Iris N Pardieck,Esmé Ti van der Gracht,Ward Vleeshouwers,Tetje C van der Sluis,J Fréderique de Graaf,Dominique Mb Veerkamp,Kees Lmc Franken,Xin Lei,Lukas van de Sand,Sjoerd H van der Burg,Marij Jp Welters,Sebastiaan Heidt,Wesley Huisman,Simon P Jochems,Martin Giera,Oliver Witzke,Aiko Pj de Vries,Andreas Kribben,Bart Everts,Benjamin Wilde,Ramon ArensPubMed 38954588
- Nature communications 14:56272023Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infection.Applications:Flow Cyt (Intra)Reactive species:MouseGraham A Heieis,Thiago A Patente,Luís Almeida,Frank Vrieling,Tamar Tak,Georgia Perona-Wright,Rick M Maizels,Rinke Stienstra,Bart EvertsPubMed 37699869
- Cell reports 40:1110322022mTORC1 signaling in antigen-presenting cells of the skin restrains CD8 T cell priming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonard R Pelgrom,Thiago A Patente,Frank Otto,Lonneke V Nouwen,Arifa Ozir-Fazalalikhan,Alwin J van der Ham,Hendrik J P van der Zande,Graham A Heieis,Ramon Arens,Bart EvertsPubMed 35793635
- iScience 24:1033122021Reduced mitochondrial respiration in T cells of patients with major depressive disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefanie Gamradt,Helge Hasselmann,Aline Taenzer,Jelena Brasanac,Victoria Stiglbauer,Arne Sattler,Max Sajitz-Hermstein,Sylwia Kierszniowska,Caren Ramien,Jan Nowacki,Lea Mascarell-Maricic,Katja Wingenfeld,Dominique Piber,Andreas Ströhle,Katja Kotsch,Friedemann Paul,Christian Otte,Stefan M GoldPubMed 34765928
- Cancer management and research 13:5683-56982021Identification of a Prognostic Index Based on a Metabolic-Genomic Landscape Analysis of Hepatocellular Carcinoma (HCC).Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Yang,Qiong Liu,Juan Zou,Yu-Kun Li,Xia XiePubMed 34295189
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested |
| Mouse | Predicted | Expected | Predicted | Predicted | Predicted |
| Rat | Predicted | Expected | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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8 products for Alternative Product
Target data
Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Alternative names
Glucose-6-phosphate 1-dehydrogenase, G6PD, G6PD
Recommended products
Rabbit Recombinant Monoclonal Glucose 6 Phosphate Dehydrogenase antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20668
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab231828 is the carrier-free version of Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
- Biological function summary
Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.
- Pathways
Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.
- Associated diseases and disorders
Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120367.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Staining on hepatocellular carcinoma (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103523.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on stroma of human liver (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunocytochemistry-immunofluorescence-img73529.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunofluorescent analysis of methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunocytochemistry-immunofluorescence-img73034.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunofluorescent analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLA cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32075.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue (left panel) and human gastric paracarcinoma (right panel) labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocaricoma, compared with weak cytoplasmic staining on the paired paracarcinoma stomach (PMID: 22012600). Both tissue sections are derived from the same patient sample. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66372.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong cytoplasmic staining on human gastric adenocarcinoma (PMID: 22012600). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunoprecipitation-img1285.jpg "Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 in HeLa whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
All lanes: Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702)
Predicted band size: 59 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120519.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat liver (PMID: 24994855, PMID: 26583321). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--flow-cytometry-intracellular-img413911.jpg "Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Glucose 6 Phosphate Dehydrogenase with Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702 at 1/400 (red) compared with an Isotype control rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] ab210702).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66372.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
![Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828), expandable thumbnail](https://content.abcam.com/products/images/glucose-6-phosphate-dehydrogenase-antibody-epr20668-bsa-and-azide-free-ab231828--immunoprecipitation-img1285.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR20668] - BSA and Azide free (ab231828)")
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