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Rabbit Recombinant Monoclonal Glucose 6 Phosphate Dehydrogenase antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target data

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

Alternative names

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Rabbit Recombinant Monoclonal Glucose 6 Phosphate Dehydrogenase antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR6292

Purification technique

Affinity purification Protein A

Dissociation constant

4.3 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525), expandable thumbnail

    Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

    All lanes: Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (AB133525) at 1/1000 dilution

    Lane 1: Molt 4 cell lysate at 10 µg

    Lane 2: A549 cell lysate at 10 µg

    Lane 3: MCF7 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Lane 5: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa

    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

    Immunohistochemistry analysis of Paraffin Embedded Human testis tissue labelling Glucose 6 Phosphate Dehydrogenase with ab133525 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling Glucose 6 Phosphate Dehydrogenase with purified ab133525 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • OI-RD Scanning - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525), expandable thumbnail

    OI-RD Scanning - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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