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AB34679

Anti-Glucose Oxidase antibody

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(1 Publication)

Rabbit Polyclonal Glucose Oxidase antibody. Suitable for IP, ELISA, Dot, WB and reacts with Aspergillus niger samples. Cited in 1 publication.

View Alternative Names

Glucose oxidase, GOD, GOx, Beta-D-glucose:oxygen 1-oxido-reductase, gox

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Aspergillus niger

Applications

ELISA, IP, WB, Dot

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Aspergillus niger": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/3500 - 1/16000", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/3500 - 1/16000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification technique
Ion exchange chromatography
Purification notes
Anti-Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose oxidase also known as GOX is an enzyme that catalyzes the oxidation of beta-D-glucose to glucono delta-lactone releasing hydrogen peroxide as a by-product. This enzyme with a molecular weight of approximately 160 kDa is commonly found in fungi like Aspergillus and Penicillium species. Glucose oxidase is predominantly expressed in the extracellular space of these organisms facilitating its role in glucose metabolism and defense mechanisms. It plays an important role in various biochemical assays including the 'glucose oxidase assay' 'glucose oxidase test' and 'glucose oxidase kit' often used for glucose quantification in diverse samples.
Biological function summary

Glucose oxidase is significant in both metabolic pathways and environmental defense. The enzyme acts as a part of a larger enzymatic reaction complex that includes peroxidase which together help in the glucose oxidase reaction to maintain cellular redox balance. Additionally the production of hydrogen peroxide by glucose oxidase serves as a chemical defense mechanism against microbial infections. This property is harnessed in technical applications making glucose oxidase a valuable tool in biotechnology for both its metabolic impacts and antimicrobial properties.

Pathways

Glucose oxidase is an important enzyme in the carbohydrate metabolism and oxidative stress response pathways. It acts by converting glucose into glucono delta-lactone which can then enter further metabolic processes. The enzyme interacts closely with other proteins particularly those involved in oxidative phosphorylation such as catalase which neutralizes the hydrogen peroxide generated during the glucose oxidase reaction. These interactions help modulate cellular energy production and maintain oxidative homeostasis highlighting its role within broader metabolic networks.

Glucose oxidase activity is relevant to conditions such as diabetes and neurodegenerative diseases. The enzyme's function in glucose monitoring tools plays a critical role in diabetes management where accurate glucose measurement is important. Additionally altered levels of hydrogen peroxide due to dysregulated glucose oxidase activity is implicated in oxidative stress which can contribute to neurodegenerative disorders like Alzheimer's disease. In these contexts the enzyme's interaction with proteins like catalase and superoxide dismutase becomes significant as these proteins aid in mitigating oxidative damage which is central to the pathology of such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glucose oxidase catalyzes the oxidation of beta-D-glucose to D-glucono-delta-lactone and hydrogen peroxide in the presence of molecular oxygen. D-glucono-delta-lactone is sequentially hydrolyzed by lactonase to D-gluconic acid, and the resulting hydrogen peroxide is hydrolyzed by catalase to oxygen and water (PubMed : 14188176, PubMed : 14257628, PubMed : 14299649, PubMed : 15450808, PubMed : 2076553, PubMed : 2406261, PubMed : 24283586, PubMed : 28631058, PubMed : 28939970, PubMed : 34500289, PubMed : 7826581, Ref.4, Ref.5). The activity shows high specificity to beta-D-glucose, with very low to no activity towards L-glucose, 2-deoxy-D-glucose, 3-deoxy-D-glucose, 4-deoxy-D-glucose, 5-deoxy-D-glucose, 6-deoxy-D-glucose, 3-O-methyl-D-glucose, 4-O-methyl-D-glucose, 6-O-methyl-D-glucose, 4,6-O-benzylidene-D-glucose, 5-thio-5-deoxy-D-glucose, D-mannose, D-allose, D-galactose, D-fructose, D-arabinose, D-xylose, trehalose, melibiose, L-mannomethylose, lactose, sucrose or 1,5-anhydro-D-glucitol (PubMed : 14188176, PubMed : 14257628, PubMed : 24283586, Ref.5).
See full target information Glucose oxidase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental immunology 194:67-78 PubMed30229863

2018

Exosomes in HNSCC plasma as surrogate markers of tumour progression and immune competence.

Applications

Unspecified application

Species

Unspecified reactive species

M-N Theodoraki,T K Hoffmann,E K Jackson,T L Whiteside
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