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AB169779

Anti-Glucose Transporter 8 antibody [EPR9477]

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(4 Publications)

Rabbit Recombinant Monoclonal Glucose Transporter 8 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications.

View Alternative Names

GLUT8, GLUTX1, SLC2A8, Glucose transporter type 8, Glucose transporter type X1, GLUT-8

3 Images
Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)
  • WB

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Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

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Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)
  • WB

Lab

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST
The observed bands are consistent with what are described in PMID : 10860996

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution

All lanes:

Human skeletal muscle lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 38 kDa

false

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)
  • WB

Lab

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

The observed bands are consistent with what are described in PMID : 10860996

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 15 µg

Lane 2:

Rat brain tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 38 kDa

false

  • Carrier free

    Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9477

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa.</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p>There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose Transporter 8 also known as GLUT8 is a member of the sugar transporter family. This protein plays a mechanical role in transferring glucose across cellular membranes. GLUT8 operates with facilitative transport ensuring glucose entry into cells. It has a molecular mass of around 55 kDa. Expression sites for GLUT8 include tissues like the testis brain and placenta. This specific transporter assists in maintaining glucose homeostasis critical for cell metabolism and energy production.
Biological function summary

GLUT8 facilitates the uptake of glucose into cells supporting cellular survival and energetic demands. It does not appear to be part of any large protein complex but operates independently to bring glucose into the intracellular environment. This functionality underpins its involvement in energy regulation influencing cellular respiration and growth by ensuring glucose availability especially in specialized tissues such as the brain and placenta.

Pathways

GLUT8 acts within the insulin signaling and glucose transport pathways. The transporter integrates with the insulin-responsive network where it responds to insulin stimulation to increase glucose uptake in cells expressing it similar to insulin-regulated transporters like GLUT4. GLUT8's functionality ensures effective glucose utilization directly linking its transport activity with energy metabolism pathways.

GLUT8's role connects to metabolic dysfunction and neurological conditions. Research shows a connection to diabetes primarily through its responsive nature to insulin which affects glucose homeostasis. Additionally GLUT8 is linked to Alzheimer's disease with potential interactions with amyloid precursor proteins and insulin signaling disruptions. These connections highlight GLUT8's relevance to neurological and energy balance disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Insulin-regulated facilitative hexose transporter that mediates the transport of glucose and fructose (By similarity). Facilitates hepatic influx of dietary trehalose, which in turn inhibits glucose and fructose influx triggering a starvation signal and hepatic autophagy through activation of AMPK and ULK1 (PubMed : 27922102). Also able to mediate the transport of dehydroascorbate.
See full target information SLC2A8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal 38:9579-9599 PubMed30232223

2018

Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Chul Shin,Carlos Cepeda,Ana María Estrada-Sánchez,Michael S Levine,Laya Hodaei,Yun Dai,Jai Jung,Amit Ganguly,Peter Clark,Sherin U Devaskar

PloS one 13:e0193583 PubMed29590129

2018

Humanin (HN) and glucose transporter 8 (GLUT8) in pregnancies complicated by intrauterine growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Janzen,Margarida Y Y Lei,Il Seok D Jeong,Amit Ganguly,Peggy Sullivan,Vladislava Paharkova,Gina Capodanno,Hiromi Nakamura,Alix Perry,Bo-Chul Shin,Kuk-Wha Lee,Sherin U Devaskar

Nutrition, metabolism, and cardiovascular diseases 26:614-622 PubMed27052924

2016

Impaired stimulation of glucose transport in cardiac myocytes exposed to very low-density lipoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

I Papageorgiou,C Viglino,M-C Brulhart-Meynet,R W James,R Lerch,C Montessuit

Antioxidants & redox signaling 22:848-70 PubMed25602171

2015

Reactive oxygen species-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Zhang,Jian-Hua Zhang,Xu-Yang Chen,Qing-Hua Hu,Ming-Xing Wang,Rui Jin,Qing-Yu Zhang,Wei Wang,Rong Wang,Lin-Lin Kang,Jin-Sheng Li,Meng Li,Ying Pan,Jun-Jian Huang,Ling-Dong Kong
View all publications
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Product promise

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