AB249523

Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Glucose Transporter 8 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

GLUT8, GLUTX1, SLC2A8, Glucose transporter type 8, Glucose transporter type X1, GLUT-8

3 Images

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Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)

This data was developed using ab169779, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (<a href='/en-us/products/primary-antibodies/glucose-transporter-8-antibody-epr9477-ab169779'>ab169779</a>) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

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Lab

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)

This data was developed using ab169779, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed bands are consistent with what are described in PMID : 10860996.

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (<a href='/en-us/products/primary-antibodies/glucose-transporter-8-antibody-epr9477-ab169779'>ab169779</a>) at 1/1000 dilution

All lanes:

Human skeletal muscle lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 38 kDa

false

Lab

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)

This data was developed using ab169779, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed bands are consistent with what are described in PMID : 10860996.

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (<a href='/en-us/products/primary-antibodies/glucose-transporter-8-antibody-epr9477-ab169779'>ab169779</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 15 µg

Lane 2:

Rat brain tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 38 kDa

false

Unconjugated

Anti-Glucose Transporter 8 antibody [EPR9477]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9477

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa." } } }

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Product details

ab249523 is the carrier-free version of ab169779.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose Transporter 8 also known as GLUT8 is a member of the sugar transporter family. This protein plays a mechanical role in transferring glucose across cellular membranes. GLUT8 operates with facilitative transport ensuring glucose entry into cells. It has a molecular mass of around 55 kDa. Expression sites for GLUT8 include tissues like the testis brain and placenta. This specific transporter assists in maintaining glucose homeostasis critical for cell metabolism and energy production.

Biological function summary

GLUT8 facilitates the uptake of glucose into cells supporting cellular survival and energetic demands. It does not appear to be part of any large protein complex but operates independently to bring glucose into the intracellular environment. This functionality underpins its involvement in energy regulation influencing cellular respiration and growth by ensuring glucose availability especially in specialized tissues such as the brain and placenta.

Pathways

GLUT8 acts within the insulin signaling and glucose transport pathways. The transporter integrates with the insulin-responsive network where it responds to insulin stimulation to increase glucose uptake in cells expressing it similar to insulin-regulated transporters like GLUT4. GLUT8's functionality ensures effective glucose utilization directly linking its transport activity with energy metabolism pathways.

GLUT8's role connects to metabolic dysfunction and neurological conditions. Research shows a connection to diabetes primarily through its responsive nature to insulin which affects glucose homeostasis. Additionally GLUT8 is linked to Alzheimer's disease with potential interactions with amyloid precursor proteins and insulin signaling disruptions. These connections highlight GLUT8's relevance to neurological and energy balance disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin-regulated facilitative hexose transporter that mediates the transport of glucose and fructose (By similarity). Facilitates hepatic influx of dietary trehalose, which in turn inhibits glucose and fructose influx triggering a starvation signal and hepatic autophagy through activation of AMPK and ULK1 (PubMed : 27922102). Also able to mediate the transport of dehydroascorbate.

See full target information SLC2A8

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise