Rabbit Recombinant Monoclonal Glucose Transporter 8 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-8-antibody-epr9477-bsa-and-azide-free-ab249523--western-blot-img87176.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)")
![Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-8-antibody-epr9477-bsa-and-azide-free-ab249523--western-blot-img25315.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)")
![Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-8-antibody-epr9477-bsa-and-azide-free-ab249523--western-blot-img64184.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (AB249523)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa. |
Species Rat | Dilution info - | Notes There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa. |
Species Human | Dilution info - | Notes There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa. |
Target data
Function
Insulin-regulated facilitative hexose transporter that mediates the transport of glucose and fructose (By similarity). Facilitates hepatic influx of dietary trehalose, which in turn inhibits glucose and fructose influx triggering a starvation signal and hepatic autophagy through activation of AMPK and ULK1 (PubMed:27922102). Also able to mediate the transport of dehydroascorbate.
Alternative names
GLUT8, GLUTX1, SLC2A8, Glucose transporter type 8, Glucose transporter type X1, GLUT-8
Recommended products
Rabbit Recombinant Monoclonal Glucose Transporter 8 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9477
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249523 is the carrier-free version of Anti-Glucose Transporter 8 antibody [EPR9477] ab169779.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glucose Transporter 8 also known as GLUT8 is a member of the sugar transporter family. This protein plays a mechanical role in transferring glucose across cellular membranes. GLUT8 operates with facilitative transport ensuring glucose entry into cells. It has a molecular mass of around 55 kDa. Expression sites for GLUT8 include tissues like the testis brain and placenta. This specific transporter assists in maintaining glucose homeostasis critical for cell metabolism and energy production.
- Biological function summary
GLUT8 facilitates the uptake of glucose into cells supporting cellular survival and energetic demands. It does not appear to be part of any large protein complex but operates independently to bring glucose into the intracellular environment. This functionality underpins its involvement in energy regulation influencing cellular respiration and growth by ensuring glucose availability especially in specialized tissues such as the brain and placenta.
- Pathways
GLUT8 acts within the insulin signaling and glucose transport pathways. The transporter integrates with the insulin-responsive network where it responds to insulin stimulation to increase glucose uptake in cells expressing it similar to insulin-regulated transporters like GLUT4. GLUT8's functionality ensures effective glucose utilization directly linking its transport activity with energy metabolism pathways.
- Associated diseases and disorders
GLUT8's role connects to metabolic dysfunction and neurological conditions. Research shows a connection to diabetes primarily through its responsive nature to insulin which affects glucose homeostasis. Additionally GLUT8 is linked to Alzheimer's disease with potential interactions with amyloid precursor proteins and insulin signaling disruptions. These connections highlight GLUT8's relevance to neurological and energy balance disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-8-antibody-epr9477-bsa-and-azide-free-ab249523--western-blot-img87176.jpg "Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523)")
Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523)
This data was developed using Anti-Glucose Transporter 8 antibody [EPR9477] ab169779, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed bands are consistent with what are described in PMID: 10860996.
All lanes: Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (Anti-Glucose Transporter 8 antibody [EPR9477] ab169779) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 15 µg
Lane 2: Rat brain tissue lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 38 kDa
![Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-8-antibody-epr9477-bsa-and-azide-free-ab249523--western-blot-img25315.jpg "Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523)")
Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523)
This data was developed using Anti-Glucose Transporter 8 antibody [EPR9477] ab169779, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed bands are consistent with what are described in PMID: 10860996.
All lanes: Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (Anti-Glucose Transporter 8 antibody [EPR9477] ab169779) at 1/1000 dilution
All lanes: Human skeletal muscle lysates at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 38 kDa
![Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-8-antibody-epr9477-bsa-and-azide-free-ab249523--western-blot-img64184.jpg "Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523)")
Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] - BSA and Azide free (ab249523)
This data was developed using Anti-Glucose Transporter 8 antibody [EPR9477] ab169779, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (Anti-Glucose Transporter 8 antibody [EPR9477] ab169779) at 1/1000 dilution
Lane 1: Human cerebellum lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com