Anti-Glucose Transporter GLUT1 antibody ab15309 is a rabbit polyclonal antibody that is used in Glucose Transporter GLUT1 IHC and immunofluorescence. Suitable for human samples.
- Tried and trusted by researchers since 2004
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- Endocrinology and metabolism (Seoul, Korea) 38:395-4052023Phloretin Ameliorates Succinate-Induced Liver Fibrosis by Regulating Hepatic Stellate Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Thuc Le,Giang Nguyen,So Young Park,Hanh Nguyen Dong,Yun Kyung Cho,Jae-Ho Lee,Seung-Soon Im,Dae-Hee Choi,Eun-Hee ChoPubMed 37533177
- Discover. Oncology 14:872023ABHD17C, a metabolic and immune-related gene signature, predicts prognosis and anti-PD1 therapy response in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeihao Zhang,Yongjie Xie,Xin Yu,Changfu Liu,Wei Gao,Wenge Xing,Tongguo SiPubMed 37273016
- Nature communications 14:28942023Alanine supplementation exploits glutamine dependency induced by SMARCA4/2-loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianbing Zhu,Zheng Fu,Shary Y Chen,Dionzie Ong,Giulio Aceto,Rebecca Ho,Jutta Steinberger,Anie Monast,Virginie Pilon,Eunice Li,Monica Ta,Kyle Ching,Bianca N Adams,Gian L Negri,Luc Choiniere,Lili Fu,Kitty Pavlakis,Patrick Pirrotte,Daina Z Avizonis,Jeffrey Trent,Bernard E Weissman,Ramon I Klein Geltink,Gregg B Morin,Morag Park,David G Huntsman,William D Foulkes,Yemin Wang,Sidong HuangPubMed 37210563
- BMC biology 21:982023Osteosarcoma tumors maintain intra-tumoral transcriptional heterogeneity during bone and lung colonization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanjana Rajan,Emily M Franz,Camille A McAloney,Tatyana A Vetter,Maren Cam,Amy C Gross,Cenny Taslim,Meng Wang,Matthew V Cannon,Alexander Oles,Ryan D RobertsPubMed 37106386
- Oncology letters 25:1252023F‑fluorodeoxyglucose positron emission tomography predicts recurrence and histological grade of extrahepatic bile duct cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakafumi Yachi,Tadashi Yoshizawa,Norihisa Kimura,Hiroko Seino,Satoko Morohashi,Shintaro Goto,Keinosuke Ishido,Hiroshi Kijima,Kenichi HakamadaPubMed 36844626
- The EMBO journal 42:e1122872023Retromer oligomerization drives SNX-BAR coat assembly and membrane constriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNavin Gopaldass,Maria Giovanna De Leo,Thibault Courtellemont,Vincent Mercier,Christin Bissig,Aurélien Roux,Andreas MayerPubMed 36644906
- International journal of molecular sciences 23:20223-Bromopyruvate Suppresses the Malignant Phenotype of Vemurafenib-Resistant Melanoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrik da Silva Vital,Murilo Bonatelli,Marina Pereira Dias,Larissa Vedovato Vilela de Salis,Mariana Tomazini Pinto,Fátima Baltazar,Silvya Stuchi Maria-Engler,Céline PinheiroPubMed 36555289
- Frontiers in cellular neuroscience 16:10651932022A human-derived neurovascular unit model to study the effects of cellular cross-talk and soluble factors on barrier integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Barberio,Aimee Withers,Yash Mishra,Pierre-Olivier Couraud,Ignacio A Romero,Babette Weksler,Róisín M OwensPubMed 36545654
- Molecular neurodegeneration 17:752022Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Wang,Minghui Wang,Suren Jeevaratnam,Cassandra Rosenberg,Tadafumi C Ikezu,Francis Shue,Sydney V Doss,Alla Alnobani,Yuka A Martens,Melissa Wren,Yan W Asmann,Bin Zhang,Guojun Bu,Chia-Chen LiuPubMed 36419137
- Molecular reproduction and development 89:540-5532022Nanoparticle-mediated transgene expression of insulin-like growth factor 1 in the growth restricted guinea pig placenta increases placenta nutrient transporter expression and fetal glucose concentrations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca L Wilson,Kristin Lampe,Mukesh K Gupta,Craig L Duvall,Helen N JonesPubMed 36094907
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes See Abreview. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
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Target data
Function
Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:18245775, PubMed:19449892, PubMed:25982116, PubMed:27078104, PubMed:10227690). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed:18245775, PubMed:19449892). Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed:10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity).
Alternative names
HepG2 glucose transporter, GLUT-1, GLUT1, SLC2A1
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Anti-Glucose Transporter GLUT1 antibody ab15309 is a rabbit polyclonal antibody that is used in Glucose Transporter GLUT1 IHC and immunofluorescence. Suitable for human samples.
- Tried and trusted by researchers since 2004
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
- Biological function summary
This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.
- Pathways
GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.
- Associated diseases and disorders
GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
This image is courtesy of a customer review submitted by Heiko LocherImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody (ab15309)
ab15309 staining Glucose Transporter GLUT1 (green) in Human red blood cells tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS-T + 1% PBS) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Red - autofluorescence of erythrocytes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody (ab15309)
ab15309 staining Glucose Transporter GLUT1 in human esophagous by Immunohistochemistry (FFPE-sections).
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab15309)
ab15309 at a 1/100 dilution staining rat cells (neural stem cells from adult subventricular zone) by Immunocytochemistry/Immunofluorescence. The cells were incubated with the antibody for 18 hours and then bound antibody was detected using a Cy3 conjugated Goat anti-rabbit IgG (H + L).
This image is courtesy of an Abreview submitted by Martin Maurer.
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody (ab15309)
ICC/IF image of ab15309 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15309, 1μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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