Rabbit Monoclonal Glucose Transporter GLUT1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunocytochemistry-immunofluorescence-img84474.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403)")
![Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--flow-cytometry-intracellular-img89752.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24721.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45962.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403)")
![Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--flow-cytometry-intracellular-img88969.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (AB252403)")
Publications
Filter by
- The Journal of clinical investigation 134:2024Inhibiting the NADase CD38 improves cytomegalovirus-specific CD8+ T cell functionality and metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNils Mülling,Felix M Behr,Graham A Heieis,Kristina Boss,Suzanne van Duikeren,Floortje J van Haften,Iris N Pardieck,Esmé Ti van der Gracht,Ward Vleeshouwers,Tetje C van der Sluis,J Fréderique de Graaf,Dominique Mb Veerkamp,Kees Lmc Franken,Xin Lei,Lukas van de Sand,Sjoerd H van der Burg,Marij Jp Welters,Sebastiaan Heidt,Wesley Huisman,Simon P Jochems,Martin Giera,Oliver Witzke,Aiko Pj de Vries,Andreas Kribben,Bart Everts,Benjamin Wilde,Ramon ArensPubMed 38954588
- Nature immunology 24:2135-21492023Harnessing CD3 diversity to optimize CAR T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRubí M-H Velasco Cárdenas,Simon M Brandl,Ana Valeria Meléndez,Alexandra Emilia Schlaak,Annabelle Buschky,Timo Peters,Fabian Beier,Bryan Serrels,Sanaz Taromi,Katrin Raute,Simon Hauri,Matthias Gstaiger,Silke Lassmann,Johannes B Huppa,Melanie Boerries,Geoffroy Andrieux,Bertram Bengsch,Wolfgang W Schamel,Susana MinguetPubMed 37932456
- Nature communications 14:56272023Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infection.Applications:Flow Cyt (Intra)Reactive species:MouseGraham A Heieis,Thiago A Patente,Luís Almeida,Frank Vrieling,Tamar Tak,Georgia Perona-Wright,Rick M Maizels,Rinke Stienstra,Bart EvertsPubMed 37699869
- Oncology letters 26:4042023Prognostic impact of the combination of HIF‑1α and GLUT1 in patients with oesophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanjie Yi,Yongqin Han,Qin Li,Runduan Lin,Jia Zhang,Yun Yang,Xueping Wang,Lin ZhangPubMed 37600334
- Cell reports 40:1110322022mTORC1 signaling in antigen-presenting cells of the skin restrains CD8 T cell priming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonard R Pelgrom,Thiago A Patente,Frank Otto,Lonneke V Nouwen,Arifa Ozir-Fazalalikhan,Alwin J van der Ham,Hendrik J P van der Zande,Graham A Heieis,Ramon Arens,Bart EvertsPubMed 35793635
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Not recommended | Expected | Predicted | Predicted |
| Rat | Not recommended | Expected | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading. |
Species Rat | Dilution info - | Notes We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading. |
Species Human | Dilution info - | Notes We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This product gave a positive signal in A549 (SLC2A1 knockout A549 cells used as a negative control) fixed with 100% methanol (5 min). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Version
2 products for Alternative Product
Target data
Function
Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:18245775, PubMed:19449892, PubMed:25982116, PubMed:27078104, PubMed:10227690). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed:18245775, PubMed:19449892). Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed:10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity).
Alternative names
HepG2 glucose transporter, GLUT-1, GLUT1, SLC2A1
Recommended products
Rabbit Monoclonal Glucose Transporter GLUT1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Alternative names
HepG2 glucose transporter, GLUT-1, GLUT1, SLC2A1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR3915
- Purification technique
Affinity purification Protein A
- Specificity
We recommend not to boil the samples after lysis to get desired WB bands.
- Dissociation constant
7.7 x 10-12 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab252403 is the carrier-free version of Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
- Biological function summary
This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.
- Pathways
GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.
- Associated diseases and disorders
GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
17 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunocytochemistry-immunofluorescence-img84474.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Immunofluorescence staining of HepG2 cells with purified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.
Alexa Fluor® 488 (Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab195359) and Alexa Fluor® 647 (Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab195020) conjugated versions are available for this clone.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--flow-cytometry-intracellular-img89752.jpg "Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at a dilution of 1/40 (red line). The secondary antibody used was Alexa Fluor®488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
Alexa Fluor®488 (Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab195359) and Alexa Fluor®647 (Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab195020) conjugated versions are available for this clone.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24721.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Immunohistochemical staining of paraffin embedded human lung carcinoma with purified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at a working dilution of 1/500. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img45962.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at a working dilution of 1/500. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--flow-cytometry-intracellular-img88969.jpg "Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Overlay histogram showing HeLa cells stained with unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137111.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human colonic adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133370.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 showing positive staining in human normal liver tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136572.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89404.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 showing positive staining in human normal breast tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80760.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 showing positive staining in human normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118350.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 showing positive staining in human kidney carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135810.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 showing negative staining in human skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10917.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 showing positive staining in human urinary bladder transitional carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80898.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Unpurified Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 showing negative staining in human normal heart tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![OI-RD Scanning - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--oi-rd-scanning-img82364.jpg "OI-RD Scanning - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
OI-RD Scanning - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427921.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
This image is courtesy of a customer review submitted by Natalie Papazian.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed human tonsil tissue staining with ab252403 at 1/500 dilution. Secondary antibody was ready to use Alexa Fluor™ Donkey anti-Rabbit IgG (H+L). Samples were incubated with the primary antibody with PBS +1% BSA for 16 hours at 4°C. Blocking was done using 5% serum for 1 hour at room temperature. Heat mediated antigen retrieval with EDTA 1mM PH8.
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunocytochemistry-immunofluorescence-img400157.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 staining SLC2A1 in wild-type A549 cells, with negative expression in SLC2A1 knockout A549 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137111.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133370.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136572.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89404.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80760.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118350.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135810.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10917.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80898.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![OI-RD Scanning - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--oi-rd-scanning-img82364.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427921.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403), expandable thumbnail](https://content.abcam.com/products/images/glucose-transporter-glut1-antibody-epr3915-bsa-and-azide-free-ab252403--immunocytochemistry-immunofluorescence-img400157.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com