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AB238050

Anti-Glucose Transporter GLUT1 antibody [GLUT1/2475]

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(2 Publications)

Mouse Monoclonal Glucose Transporter GLUT1 antibody. Suitable for IHC-P, PepArr and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC2A1 aa 200-350.

View Alternative Names

GLUT1, SLC2A1, HepG2 glucose transporter, GLUT-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [GLUT1/2475] (AB238050)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [GLUT1/2475] (AB238050)

Formalin-fixed, paraffin-embedded human tongue tissue stained for Glucose Transporter GLUT1 using ab238050 at 1 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [GLUT1/2475] (AB238050)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [GLUT1/2475] (AB238050)

Formalin-fixed, paraffin-embedded human bladder carcinoma tissue stained for Glucose Transporter GLUT1 using ab238050 at 1 μg/ml in immunohistochemical analysis.

Peptide Array - Anti-Glucose Transporter GLUT1 antibody [GLUT1/2475] (AB238050)
  • PepArr

Supplier Data

Peptide Array - Anti-Glucose Transporter GLUT1 antibody [GLUT1/2475] (AB238050)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238050.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GLUT1/2475

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

PepArr, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC2A1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

P11166

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "PepArr" : {"fullname" : "Peptide Array", "shortname":"PepArr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "PepArr-species-checked": "testedAndGuaranteed", "PepArr-species-dilution-info": "", "PepArr-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
Biological function summary

This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.

Pathways

GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.

GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed : 10227690, PubMed : 10954735, PubMed : 18245775, PubMed : 19449892, PubMed : 25982116, PubMed : 27078104, PubMed : 32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed : 18245775, PubMed : 19449892). Most important energy carrier of the brain : present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed : 10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).
See full target information SLC2A1

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 8:806650 PubMed35096971

2022

Accumulated ROS Activates HIF-1α-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Liang,Shuohui Dong,Wenwen Liu,Man Wang,Shanshan Tian,Yu Ai,Haibo Wang

Cancer management and research 13:1099-1111 PubMed33574707

2021

Anti-Tumor Effect of Celastrol on Hepatocellular Carcinoma by the circ_SLIT3/miR-223-3p/CXCR4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hailong Si,Huiling Wang,Haijuan Xiao,Yu Fang,Zhaoli Wu
View all publications

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