Name: Anti-Glucose Transporter GLUT1 antibody [SP168]
SKU: ab150299
Rating: 3 (3 reviews)

Knockout Tested Rabbit Recombinant Monoclonal Glucose Transporter GLUT1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Mouse, Human samples. Cited in 20 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SP168] (AB150299), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt (Intra)	WB	ICC/IF
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Expected	Expected
Rat	Predicted	Predicted	Predicted	Predicted
Chicken	Predicted	Predicted	Predicted	Predicted
Cow	Predicted	Predicted	Predicted	Predicted
Pig	Predicted	Predicted	Predicted	Predicted
Rabbit	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/200	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/200	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Chicken, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Chicken, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Chicken, Cow, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Chicken, Cow, Pig	Dilution info -	Notes -

Associated Products

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Target data

See full target information SLC2A1

Function

Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:10227690, PubMed:10954735, PubMed:18245775, PubMed:19449892, PubMed:25982116, PubMed:27078104, PubMed:32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed:18245775, PubMed:19449892). Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed:10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).

Alternative names

GLUT1, SLC2A1, HepG2 glucose transporter, GLUT-1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Glucose Transporter GLUT1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Mouse, Human samples. Cited in 20 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: SP168
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.

Biological function summary

This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.

Pathways

GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.

Associated diseases and disorders

GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.

Product promise

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9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse lung tissue sections labeling Glucose Transporter GLUT1 with ab150299 at 1/200 dilution (1.24 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling Glucose Transporter GLUT1 with ab150299 at 1/200 dilution (1.24 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Glucose Transporter GLUT1 with ab150299 at 1/200 dilution (1.24 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Glucose Transporter GLUT1 with purified ab150299 at 1/100 (2.5 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Glucose Transporter GLUT1 with purified ab150299 at 1/200 dilution (1.24 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung carcinoma tissue labelling Glucose Transporter GLUT1 with ab150299 at 1/200 dilution.
Western blot - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
All lanes: Western blot - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299) at 1/200 dilution
All lanes: HepG2 cell lysate
Predicted band size: 54 kDa
Western blot - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
Western blot: Anti-Glucose Transporter GLUT1 antibody [SP168] staining at 1/200 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab150299 was shown to bind specifically to Glucose Transporter GLUT1. A band was observed at 50-300 kDa in wild-type HepG2 cell lysates with no signal observed at this size in SLC2A1 knockout cell line Human SLC2A1 (Glucose Transporter GLUT1) knockout Hep G2 cell line ab280797 (knockout cell lysate ab284224). To generate this image, wild-type and SLC2A1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299) at 1/200 dilution
Lane 1: Wild-type HepG2 cell lysate at 20 µg
Lane 2: SLC2A1 knockout HepG2 cell lysate at 20 µg
Lane 3: A549 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Observed band size: 50-300 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [SP168] (ab150299)
HCT116 WT, SLC2A1 KO were labelled with a green dye. SLC2A1 KOOE cells were labelled with a green and a red dye. SLC2A1 expression was induced with doxycycline in KOOE cells. WT and KO cells were stained with ab150299 and with Alexa-fluor 594 coupled secondary antibody. KOOE cells were stained with: i) ab150299 and with Alexa-fluor 594 coupled secondary antibody; ii) anti-HA-Tag antibody and with Alexa-fluor 488 antibody. Acquisition of the green (HA-Tag in KOOE) and red (antibody staining in WT, KO and KOOE) channels was performed. Representative images of the green and red channel are shown. The antibody used and tested dilution are as follows: ab150299 at 1/50, 1/100 and 1/200.
This image was provided, with thanks, by RESOLUTE (re-solute.eu).