Anti-Glucose Transporter GLUT2 antibody [5D1]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT2 antibody [5D1] (AB85715)

ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.

• Flow Cyt

Unknown

Flow Cytometry - Anti-Glucose Transporter GLUT2 antibody [5D1] (AB85715)

Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

Unknown

Western blot - Anti-Glucose Transporter GLUT2 antibody [5D1] (AB85715)

All lanes:

Western blot - Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/mL

All lanes:

Human pancreas tissue lysate - total protein (<a href='/en-us/products/unavailable/human-pancreas-tissue-lysate-total-protein-ab29816'>ab29816</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

true

Exposure time: 8min