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Rabbit Polyclonal Glucose Transporter GLUT2 antibody. Suitable for WB, I-ELISA, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 54 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (AB54460), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link P12336

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBI-ELISAICC/IF
Human
Expected
Expected
Tested
Mouse
Expected
Expected
Predicted
Rat
Expected
Expected
Predicted

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose (PubMed:16186102, PubMed:23396969, PubMed:28083649, PubMed:8027028, PubMed:8457197). Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell (PubMed:8027028). May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney (PubMed:3399500). Also able to mediate the transport of dehydroascorbate (PubMed:23396969).

Alternative names

Recommended products

Rabbit Polyclonal Glucose Transporter GLUT2 antibody. Suitable for WB, I-ELISA, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 54 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link P12336
Purification technique

Affinity purification Immunogen

Specificity

This antibody recognizes the ~53-61 kDa GLUT2 protein. It does not recognize other GLUT isoforms.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Following initial thaw, aliquot and freeze (-20°C).

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The glucose transporter GLUT2 also referred to as GLUT 2 GLUT-2 or GLUT2 transporter plays an important role in the movement of glucose across cell membranes. Mechanically it acts as a facilitative transporter allowing glucose and other monosaccharides to enter and exit cells. GLUT2 has a molecular weight of about 55 kDa. High expression of GLUT2 occurs in the liver pancreas kidney and intestines where it facilitates bidirectional glucose transport to maintain glucose balance.

Biological function summary

The role of this transporter is important for glucose sensing and regulation. It is not part of any known complex but operates efficiently as a single unit within its membrane environment. In the pancreas GLUT2 detects blood glucose levels and initiates insulin release from beta cells. In the liver it enables glucose uptake during periods of high blood glucose and facilitates glucose production when blood glucose levels drop. By performing these functions GLUT2 helps maintain glucose homeostasis in the body.

Pathways

GLUT2 is an essential component of glucose homeostasis and nutrient sensing pathways. It links to the insulin signaling pathway by facilitating insulin secretion in response to increased blood glucose. Furthermore GLUT2 associates with kinases such as AMPK which modulate cellular energy balance. The synergistic activity between GLUT2 and insulin signaling pathways highlights its central role in metabolic processes.

Associated diseases and disorders

Altered GLUT2 function has significant implications. Mutations or deficiencies in GLUT2 can lead to conditions like Fanconi-Bickel syndrome a rare glycogen storage disorder. This transporter has been connected to diabetes as well due to its involvement in insulin secretion regulation. In both these instances GLUT2 interacts with other proteins such as glucokinase in the pancreas and liver highlighting its importance in disease pathology.

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (ab54460), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (ab54460)

    ICC/IF image of ab54460 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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