AB54460

Anti-Glucose Transporter GLUT2 antibody

Name: Anti-Glucose Transporter GLUT2 antibody (ab54460) | Abcam
Brand: Abcam Limited
SKU: AB54460
Price: 450 USD
Availability: InStock
Rating: 4 (9 reviews)

(9 Reviews)

(69 Publications)

Anti-Glucose Transporter GLUT2 antibody (ab54460) is a rabbit polyclonal antibody detecting Glucose Transporter GLUT2 in Western Blot, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Over 50 publications
- Trusted since 2007

View Alternative Names

GLUT2, SLC2A2, GLUT-2

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (AB54460)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (AB54460)

ICC/IF image of ab54460 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

I-ELISA, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes the ~53-61 kDa GLUT2 protein. It does not recognize other GLUT isoforms.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Glucose Transporter GLUT2 antibody (ab54460) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-Glucose Transporter GLUT2 (ab54460) was first used in a scientific publication in 2007 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Glucose Transporter GLUT2 (ab54460) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glucose transporter GLUT2 also referred to as GLUT 2 GLUT-2 or GLUT2 transporter plays an important role in the movement of glucose across cell membranes. Mechanically it acts as a facilitative transporter allowing glucose and other monosaccharides to enter and exit cells. GLUT2 has a molecular weight of about 55 kDa. High expression of GLUT2 occurs in the liver pancreas kidney and intestines where it facilitates bidirectional glucose transport to maintain glucose balance.

Biological function summary

The role of this transporter is important for glucose sensing and regulation. It is not part of any known complex but operates efficiently as a single unit within its membrane environment. In the pancreas GLUT2 detects blood glucose levels and initiates insulin release from beta cells. In the liver it enables glucose uptake during periods of high blood glucose and facilitates glucose production when blood glucose levels drop. By performing these functions GLUT2 helps maintain glucose homeostasis in the body.

Pathways

GLUT2 is an essential component of glucose homeostasis and nutrient sensing pathways. It links to the insulin signaling pathway by facilitating insulin secretion in response to increased blood glucose. Furthermore GLUT2 associates with kinases such as AMPK which modulate cellular energy balance. The synergistic activity between GLUT2 and insulin signaling pathways highlights its central role in metabolic processes.

Altered GLUT2 function has significant implications. Mutations or deficiencies in GLUT2 can lead to conditions like Fanconi-Bickel syndrome a rare glycogen storage disorder. This transporter has been connected to diabetes as well due to its involvement in insulin secretion regulation. In both these instances GLUT2 interacts with other proteins such as glucokinase in the pancreas and liver highlighting its importance in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose (PubMed : 16186102, PubMed : 23396969, PubMed : 28083649, PubMed : 8027028, PubMed : 8457197). Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell (PubMed : 8027028). May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney (PubMed : 3399500). Also able to mediate the transport of dehydroascorbate (PubMed : 23396969).

See full target information SLC2A2

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Publications (69)

Recent publications for all applications. Explore the full list and refine your search

Veterinary sciences 12: PubMed41012809

2025

Effects of Maternal Nutrition and One-Carbon Metabolite Supplementation on Fetal Jejunal Morphology and Hexose Transporter Expression in Beef Cattle.

Applications

Unspecified application

Species

Unspecified reactive species

Mojtaba Daneshi,Pawel P Borowicz,Virginia Montgomery,Yssi L Entzie,Jessica G Syring,Layla E King,Kazi Sarjana Safain,Muhammad Anas,Lawrence P Reynolds,Alison K Ward,Carl R Dahlen,Matthew S Crouse,Joel S Caton

BMJ open diabetes research & care 13: PubMed40716951

2025

Glucose absorption in the duodenum is modulated by an estrogen receptor α-dependent regulation of glucose transporter functional expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Ding,Xiaoxu Yang,Weixi Shan,Jingyu Xu,Qian Du,Changmei Chen,Qiushi Liao,Jun Lou,Zhe Jin,Mingkai Chen,Rui Xie

Nature communications 16:4190 PubMed40328784

2025

Astrocytic GLUT1 deletion in adult mice enhances glucose metabolism and resilience to stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Laetitia Thieren,Henri S Zanker,Jeanne Droux,Urvashi Dalvi,Matthias T Wyss,Rebecca Waag,Pierre-Luc Germain,Lukas M von Ziegler,Zoe J Looser,Ladina Hösli,Luca Ravotto,E Dale Abel,Johannes Bohacek,Susanne Wegener,L Felipe Barros,Mohamad El Amki,Bruno Weber,Aiman S Saab

Biomedicines 13: PubMed40002681

2025

The Abnormal Expression of Tubular SGLT2 and GULT2 in Diabetes Model Mice with Malocclusion-Induced Hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Koichiro Kajiwara,Sachio Tamaoki,Yoshihiko Sawa

The Journal of clinical investigation 135: PubMed39932789

2025

Ablating VHL in rod photoreceptors modulates RPE glycolysis and improves preclinical model of retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Salvatore Marco Caruso,Xuan Cui,Brian M Robbings,Noah Heapes,Aykut Demirkol,Bruna Lopes Da Costa,Daniel T Hass,Peter Mj Quinn,Jianhai Du,James B Hurley,Stephen H Tsang

Diabetes & metabolism journal 49:194-209 PubMed39721589

2024

Kidney Gastrin/CCKBR Attenuates Type 2 Diabetes Mellitus by Inhibiting SGLT2-Mediated Glucose Reabsorption through Erk/NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Zhang,Yuhan Zhang,Yang Shi,Dou Shi,Min Niu,Xue Liu,Xing Liu,Zhiwei Yang,Xianxian Wu

Genetics and molecular biology 47:e20230281 PubMed39535164

2024

Expression of the C-allele of intronic rs8192675 in SLC2A2 is associated with improved glucose response to metformin.

Applications

Unspecified application

Species

Unspecified reactive species

Wanjun Wang,Suying Chen,Yilei Jiang,Jianhong Ji,Ruochen Cong

Chinese medicine 19:128 PubMed39285464

2024

The improvement of modified Si-Miao granule on hepatic insulin resistance and glycogen synthesis in type 2 diabetes mellitus involves the inhibition of TNF-α/JNK1/IRS-2 pathway: network pharmacology, molecular docking, and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Zebiao Cao,Xianzhe Wang,Zhili Zeng,Zhaojun Yang,Yuping Lin,Lu Sun,Qiyun Lu,Guanjie Fan

Nature neuroscience 27:1934-1944 PubMed39251890

2024

Oligodendroglial fatty acid metabolism as a central nervous system energy reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Ebrahim Asadollahi,Andrea Trevisiol,Aiman S Saab,Zoe J Looser,Payam Dibaj,Reyhane Ebrahimi,Kathrin Kusch,Torben Ruhwedel,Wiebke Möbius,Olaf Jahn,Jun Yup Lee,Anthony S Don,Michelle-Amirah Khalil,Karsten Hiller,Myriam Baes,Bruno Weber,E Dale Abel,Andrea Ballabio,Brian Popko,Celia M Kassmann,Hannelore Ehrenreich,Johannes Hirrlinger,Klaus-Armin Nave

Metabolites 14: PubMed38786744

2024

Diabetes Causes Significant Alterations in Pulmonary Glucose Transporter Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Allison Campolo,Zahra Maria,Véronique A Lacombe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter GLUT2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (AB54460)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (69)

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