AB236145

Anti-Glucose Transporter GLUT2 antibody [EPR16550] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Glucose Transporter GLUT2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GLUT2, SLC2A2, GLUT-2

1 Images

Supplier Data

Western blot - Anti-Glucose Transporter GLUT2 antibody [EPR16550] - BSA and Azide free (AB236145)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192599).

All lanes:

Western blot - Anti-Glucose Transporter GLUT2 antibody [EPR16550] (<a href='/en-us/products/primary-antibodies/glucose-transporter-glut2-antibody-epr16550-ab192599'>ab192599</a>) at 1/10000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human pancreas lysate at 20 µg

Lane 3:

Human fetal kidney lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

true

Unconjugated

Anti-Glucose Transporter GLUT2 antibody [EPR16550]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16550

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab236145 is the carrier-free version of ab192599.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glucose transporter GLUT2 also referred to as GLUT 2 GLUT-2 or GLUT2 transporter plays an important role in the movement of glucose across cell membranes. Mechanically it acts as a facilitative transporter allowing glucose and other monosaccharides to enter and exit cells. GLUT2 has a molecular weight of about 55 kDa. High expression of GLUT2 occurs in the liver pancreas kidney and intestines where it facilitates bidirectional glucose transport to maintain glucose balance.

Biological function summary

The role of this transporter is important for glucose sensing and regulation. It is not part of any known complex but operates efficiently as a single unit within its membrane environment. In the pancreas GLUT2 detects blood glucose levels and initiates insulin release from beta cells. In the liver it enables glucose uptake during periods of high blood glucose and facilitates glucose production when blood glucose levels drop. By performing these functions GLUT2 helps maintain glucose homeostasis in the body.

Pathways

GLUT2 is an essential component of glucose homeostasis and nutrient sensing pathways. It links to the insulin signaling pathway by facilitating insulin secretion in response to increased blood glucose. Furthermore GLUT2 associates with kinases such as AMPK which modulate cellular energy balance. The synergistic activity between GLUT2 and insulin signaling pathways highlights its central role in metabolic processes.

Altered GLUT2 function has significant implications. Mutations or deficiencies in GLUT2 can lead to conditions like Fanconi-Bickel syndrome a rare glycogen storage disorder. This transporter has been connected to diabetes as well due to its involvement in insulin secretion regulation. In both these instances GLUT2 interacts with other proteins such as glucokinase in the pancreas and liver highlighting its importance in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Facilitative hexose transporter that mediates the transport of glucose, fructose and galactose (PubMed : 16186102, PubMed : 23396969, PubMed : 28083649, PubMed : 8027028, PubMed : 8457197). Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell (PubMed : 8027028). May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney (PubMed : 3399500). Also able to mediate the transport of dehydroascorbate (PubMed : 23396969).

See full target information SLC2A2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:105667 PubMed36624844

2023

EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Tang,Wei Wei,John M Snowball,Ernesto S Nakayasu,Sheila M Bell,Charles Ansong,Xinhua Lin,Jeffrey A Whitsett

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter GLUT2 antibody [EPR16550] - BSA and Azide free

Western blot - Anti-Glucose Transporter GLUT2 antibody [EPR16550] - BSA and Azide free (AB236145)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise